31 results about "Aureomycine" patented technology

The invention discloses a dual-emission molecular imprinting ratiometric fluorescence sensor, a preparation method thereof and application of the dual-emission molecular imprinting ratiometric fluorescence sensor in aureomycin detection, and belongs to the technical field of chemical sensors. The preparation method of the sensor comprises the following steps: preparing silicon dioxide-quantum dot composite nanoparticles, and preparing a molecularly imprinted polymer by taking salicylamide as a template molecule; and mixing the molecularly imprinted polymer and the silicon dioxide-quantum dot composite nanoparticles in an HEPES buffer solution by adopting a post-mixing strategy to construct the dual-emission molecularly imprinted ratiometric fluorescence sensor. The double-emission molecular imprinting ratiometric fluorescence sensor has the beneficial effects that the double-emission molecular imprinting ratiometric fluorescence sensor provided by the invention is rich in fluorescence color change, rapid, high-sensitivity and visual detection of aureomycin can be realized, the whole detection process is simple, convenient and rapid, and the double-emission molecular imprinting ratiometric fluorescence sensor is suitable for being widely popularized in practical application.

The invention discloses a nucleic acid aptamer TET-HeR of tetracycline and application of the nucleic acid aptamer TET-HeR, and belongs to the technical field of nucleic acid aptamers. The nucleic acid aptamer TET-HeR of the tetracycline is a combination of an aptamer TET-A1 and an aptamer TET-C1, the nucleotide sequence of the aptamer TET-A1 is as shown in SEQ ID NO.5, and the nucleotide sequence of the aptamer TET-C1 is as shown in SEQ ID NO.7. The nucleic acid aptamer TET-HeR of the tetracycline is a combination of the aptamer TET-A1 and the aptamer TET-C1. The invention further discloses the application of the nucleic acid aptamer TET-HeR of the tetracycline in detection of the tetracycline. The affinity and dissociation constant of the nucleic acid aptamer TET-HeR of the tetracycline and the tetracycline is 62.7 nM, and the nucleic acid aptamer TET-HeR of the tetracycline has high affinity; the compound has no obvious affinity to tetracycline analogues such as aureomycin and doxycycline, has weak affinity to oxytetracycline, and has good specificity; the method can be used for specific detection of tetracycline.

The present application belongs to the technical field of microbial fermentation, and particularly relates to a fermentation method for increasing the titer growth rate of aureomycin after 90 hours of fermentation and application thereof. The present application achieves the effect of comprehensively regulating the fermentation process of aureomycin by regulating the seed inoculation amount of Streptomyces aureofaciens, the total sugar concentration after seed tank depletion, the seed age and pH value after seed transfer, the ratio of slow-acting organic nitrogen to readily available organic nitrogen in the fermentation medium, the total sugar concentration after fermentation tank depletion and the total sugar concentration in the fermentation process, thereby increasing the titer growth rate of aureomycin after 90 hours of fermentation. Further, the method can control the amount of sugar supplement in the fermentation process to a certain extent without being too high, which is conducive to reducing material consumption, while avoiding premature autolysis of mycelium in the fermentation process, which is conducive to reducing the difficulty and cost of sewage treatment. In addition, the method is simple to operate, has strong popularization, has no side effects on fermentation production and product quality, and achieves good economic and ecological benefits.

The invention discloses a MnO2-EDTA (Ethylene Diamine Tetraacetic Acid) catalyst as well as a preparation method and application thereof, and belongs to the technical field of advanced oxidation treatment, and the preparation method of the MnO2-EDTA catalyst comprises the steps of material preparation, hydrothermal reaction and post-treatment. The MnO2-EDTA catalyst is prepared according to the preparation method disclosed by the invention. The application refers to the application of the MnO2-EDTA catalyst in degradation of chlortetracycline hydrochloride. The EDTA ligand is introduced to the surface of MnO2, so that the degradation efficiency of the catalyst on chlortetracycline hydrochloride is remarkably enhanced, the catalytic activity and stability are improved, meanwhile, the preparation process is safe, simple, convenient and efficient, and potential environment purification application prospects are achieved.

The invention belongs to the technical field of environmental microorganisms and sewage treatment, and particularly relates to bacillus parabarisiformis and application of the bacillus parabarisiformis in anti-wastewater treatment. The bacillus paramycoides (BCTC-2) is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.35352, and the preservation date is July 23, 2025, and the preservation number is CGMCC No.35352. The bacillus paramycoides (BCTC-2) has the advantages that the bacillus paramycoides (BCTC-2) is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.35352; the strain can be used for actual antibiotic-containing wastewater treatment, can reduce environmental pollution caused by antibiotic residues, provides a theoretical basis for industrial application of aureomycin efficient degrading bacteria, is beneficial to enriching a strain resource library of the aureomycin degrading bacteria, and provides an effective biodegradation method for pollution treatment of antibiotics in the environment.

The application provides bacillus velezensis LW56-4 and application thereof. The bacillus velezensis is bacillus velezensis LW56-4, the classification name is Bacillus velezensis , and the preservation address is China.Wuhan.Wuhan University, and the preservation number is CCTCC NO: M 20251875. The bacillus velezensis LW56-4 has broad-spectrum antibacterial property, can reduce animal diarrhea, promote animal gastrointestinal development and improve antioxidant capacity, can effectively promote feed conversion and promote animal growth, and is an ideal substitute for aureomycin and other antibiotics.

The invention provides bacillus velezensis LW56-4 and an application of the bacillus velezensis LW56-4. The bacillus velezensis is bacillus velezensis LW56-4, the classification name is bacillus velezensis, the bacillus velezensis is preserved in the China Center for Type Culture Collection on August 22, 2025, the preservation address is Wuhan University, Wuhan, China, and the preservation number is CCTCC NO: M 20251875. The bacillus velezensis LW56-4 has a broad-spectrum antibacterial property, can relieve animal diarrhea, promote gastrointestinal tract development of animals and improve oxidation resistance, can also effectively promote feed conversion and promote animal growth, and is an ideal substitute for antibiotics such as aureomycin and the like.

The application relates to the technical field of veterinary drugs, and particularly discloses aureomycin soluble powder and a preparation method thereof. The aureomycin soluble powder is composed of the following components in mass percentage: aureomycin phosphate salt 20-55%, chelating agent 0.5-2.5%, penetration promoter 0.5-2.5%, and organic acid carrier in residual amount; the aureomycin phosphate salt is mainly obtained by chemically modifying hydrochloric acid aureomycin with chlorophosphite di-tert-butyl ester, and then reacting with an alkali to form a salt. According to the application, the aureomycin is chemically modified into a phosphate salt, and the chelating agent and the penetration promoter are used in cooperation, so that the key problems of poor water solubility and low absorption rate are fundamentally solved, the solubility is improved by an order of magnitude, the oral bioavailability is significantly improved, and meanwhile, the complicated super-micro pulverization treatment required by a traditional process is avoided.

The present application relates to the field of nanomaterials and analytical detection technology, in particular to a reagent for rapid detection of aureomycin and a rapid detection method of aureomycin. After adding 3-mercaptopropionic acid modified AgInS2 quantum dots to aureomycin, the fluorescence intensity and color of AgInS2 QDs change due to electrostatic interaction and band gap transition, thereby constructing a fluorescence colorimetric sensor to realize the ultra-sensitive detection of aureomycin. In order to realize the on-site detection of aureomycin, a portable device equipped with a smartphone and integrated with the AgInS2 QDs sensor is also designed. The cloud server data analysis system based on machine learning algorithm is integrated into the smartphone, which is convenient for color data collection, correction, interpretation and display. The present application utilizes the function of modern smartphones to capture and process fluorescence color signals, thereby providing a user-friendly on-site detection solution for aureomycin detection.

The invention belongs to the technical field of antibiotic production, and particularly relates to a method for increasing the yield of an aureomycin premix. The method comprises the following steps: sampling aureomycin fermentation liquor, detecting the titer of the fermentation liquor and the dry matter of the fermentation liquor, calculating the use amount of calcium carbonate according to the preparation specification of the aureomycin premix to be produced, the volume of the fermentation liquor and the actual yield value, and adding calcium hydrophosphate and ethoxyquin according to the corresponding proportion to obtain the aureomycin calcium salt complex. And carrying out plate frame filtration and flash evaporation drying to obtain the aureomycin premix. According to the invention, the stability of the aureomycin calcium salt is effectively improved, and the yield of the aureomycin premix is improved. The method is simple in technological process, controllable in operation, stable in product quality and low in production cost.

The invention relates to the technical field of synthetic biology, and discloses a recombinant plasmid and a recombinant bacterium for producing nortetracycline as well as a construction method and application of the recombinant plasmid and the recombinant bacterium. In order to heterologously produce the nortetracycline, the invention provides a recombinant plasmid for producing the nortetracycline, a starting plasmid of the recombinant plasmid is pOxy2, and the recombinant plasmid is obtained by knocking out oxyS and oxyR genes in the starting plasmid pOxy2, then knocking out oxyF genes and introducing ctcN and ctcM genes in an aureomycin biosynthesis pathway to the position of an oxyF original reading frame. The recombinant plasmid prepared by the invention is used for selecting a host which is rapid in growth and simple and convenient in genetic manipulation as a nortetracycline industrial production strain, so that biological synthesis of nortetracycline is realized, and no other tetracycline by-products exist.

The invention belongs to the technical field of pharmaceutical preparations, and particularly relates to a compound ointment preparation for treating fungal infection and a preparation method and application thereof. According to the compound ointment preparation, chlortetracycline hydrochloride, econazole nitrate and triamcinolone acetonide compound serve as core components, no chemical incompatibility exists among chlortetracycline hydrochloride, econazole nitrate and triamcinolone acetonide, and comprehensive treatment on external auditory canal mycosis is achieved through the pharmacological synergistic effect. Meanwhile, adaptive plant essential oil is specially selected, and the proportion of active ingredients is optimized, so that the compound ointment preparation can effectively control bacteria, fungi and inflammation, the hormone amount is obviously reduced, and side effects are reduced.

The invention discloses a silver nanocluster with red emission and detection and antibacterial properties thereof, the silver nanocluster is prepared by adopting dihydrolipoic acid as a ligand and sodium borohydride as a reducing agent through an optimized synthesis process, the quantum yield is as high as 14.8%, the fluorescence lifetime is 7.91 microseconds, and the silver nanocluster has excellent fluorescence characteristics and stability. High-selectivity detection of the heavy metal ion Ni < 2 + > can be realized based on a fluorescence resonance energy transfer mechanism, and the detection limit is as low as 68 nM. Meanwhile, four types of tetracycline antibiotics (TCs) of oxytetracycline (OTC), tetracycline (TC), doxycycline (DOX) and aureomycin (CTC) are recognized through specific ratio fluorescence response. The compound has an efficient inhibition effect on common pathogenic bacteria such as gram-positive bacteria and gram-negative bacteria, and the bacteriostasis rate reaches 80% or above. The hydrogel dressing prepared by compounding AgNCs and a natural polymer material sodium alginate can effectively inhibit wound infection and promote tissue regeneration and wound healing.

The invention relates to the technical field of antibiotic production, in particular to a production process of an aureomycin premix. The invention provides a production process of an aureomycin premix, which comprises the following specific steps: adding aureomycin fermentation liquor into a composite carrier, mixing, and carrying out plate frame filtration to obtain a filter cake with the water content of 50%; crushing the filter cake to obtain a wet material, adding aureomycin powder into the wet material, and reducing the water content to be less than 40%; adding an adhesive into the wet material and uniformly mixing; granulating the wet material by using a granulator, and then collecting a wet granular material; and drying the wet granulated material in a fluidized bed, and screening the dried material to obtain a 20-80-mesh material. By optimizing the process, the particle yield of the aureomycin premix is remarkably improved, meanwhile, powder residues are greatly reduced, efficient recycling of the screened powder is achieved, and raw material loss is reduced.

The invention belongs to the technical field of aureomycin production, and particularly relates to an aureomycin fermentation culture medium, an aureomycin whole-stage culture medium, a preparation method and application of the aureomycin fermentation culture medium and an aureomycin fermentation culture method. The aureomycin fermentation culture medium provided by the invention comprises the following components: corn starch, peanut cake powder, soybean cake powder, corn steep liquor dry powder, yeast powder, protein powder, beer residues, magnesium sulfate, monopotassium phosphate, calcium carbonate, ammonium sulfate, sodium chloride, manganese sulfate, ammonium chloride, amylase, soybean oil and water, and the effective substance content of each raw material is limited. According to the aureomycin fermentation culture medium provided by the invention, the total amount of elements such as C, N and P and the content of effective substances in the raw materials are suitable for aureomycin fermentation requirements, and the quality difference of the culture medium caused by content fluctuation of the effective elements or substances among batches of the raw materials can be avoided. According to the method, the raw material input and waste are reduced, the raw material utilization rate is increased, the fluctuation of the fermentation efficiency between batches of aureomycin fermentation is reduced, and the overall quality of the product is improved.

The application discloses a method for improving egg quality by using peony and a feed additive, and belongs to the field of animal husbandry. The method for improving egg quality comprises adding 0.75-1.5% of peony stem and leaf powder in the basic daily ration of laying hens. The method can significantly improve the production performance of laying hens in the later production stage, and the effect is better than that of aureomycin. Meanwhile, the method can also significantly enhance the antioxidation ability of the body, improve the enrichment of MUFA, gamma-linolenic acid and arachidonic acid in eggs, and has the potential to replace feed antibiotics.

The application discloses a kind of over rumen aureomycin premix for sheep digestive tract infection and preparation method, including capsule material and aureomycin premix, wherein, capsule material mass percentage is 45%-55%, and aureomycin premix mass accounts for 45%-55% of total mass.The premix of the application can avoid the degradation of aureomycin in sheep rumen, ensure sufficient active drug to reach intestinal lesions, and has excellent antibacterial and antidiarrheal effects on lamb dysentery, growing sheep stress bacterial enteritis and adult sheep digestive tract secondary bacterial infection, while reducing the dosage and reducing the risk of drug residues.

The invention relates to the technical field of fluorescence sensing and the field of antibiotic fluorescence detection, in particular to a cadmium (II) MOFs (Metal-Organic Frameworks) material as well as a preparation method and application thereof in antibiotic detection. A cadmium (II) MOFs material [Cd (cpt) 2H2O] n can be prepared by adopting a one-pot hydrothermal reaction, the molecular formula is C56H38CdN6O7, and Hcpt is 4 '-(4-(4-carboxyl phenoxy) phenyl)-4, 2': 6 ', 4' '-terpyridyl. The preparation method has the advantages of mild conditions, simplicity and convenience in operation, high yield, good repeatability and the like. The cadmium (II) MOFs material in the prepared cadmium element coordination polymer has good structural stability and fluorescence characteristics and shows high selectivity and sensitivity to tetracycline antibiotics such as tetracycline hydrochloride, aureomycin and doxycycline (THC, CTC and DOX) in antibiotic detection, and the concentration of the tetracycline antibiotics can be preferentially detected through a fluorescence quenching phenomenon.

The present application relates to a recombinant vector, a recombinant strain, a kit and its use and a method for producing aureomycin. The recombinant vector comprises: a promoter selected from a kasOp* promoter or an ermEp* promoter; a regulatory gene downstream of the promoter, the regulatory gene comprising: at least one of ctc1, ctcB and ctcS; and the recombinant strain comprises the above-mentioned recombinant vector. The recombinant vector of the present application can overexpress the regulatory gene in the aureomycin-producing strain, thereby achieving high-yield aureomycin production and facilitating the large-scale production of aureomycin.

The invention belongs to the technical field of animal feed, and particularly relates to a natural plant feed raw material capable of replacing aureomycin and a preparation method of the natural plant feed raw material. The traditional Chinese medicine composition is prepared from the following natural plant raw materials in parts by weight: 15 to 25 parts of radix astragali seu hedysari, 10 to 20 parts of fructus crataegi, 10 to 18 parts of fructus hippophae, 8 to 15 parts of fructus schisandrae chinensis, 8 to 15 parts of herba portulacae, 6 to 12 parts of flos lonicerae, 10 to 18 parts of radix codonopsis and 6 to 12 parts of macleaya cordata. The natural plant feed raw materials are from pure traditional Chinese medicines / natural plants, are free of antibiotics and chemical additives, and are used for replacing aureomycin to serve as feed additives or premix for fattening monogastric / ruminants such as pigs, cattle, sheep and horses. The natural plant feed raw materials are prepared by adopting an integrated innovative process of directional enzymolysis pretreatment, ultrasonic-microwave synergistic extraction, green entrainer modified supercritical COextraction, lipidosome and nano-composite embedding, directional enrichment and efficient extraction of active ingredients and targeted release of intestinal tracts are realized, and the extraction rate of the active ingredients is improved compared with that of a traditional process.

This invention discloses a method for detecting the bioavailability of antibiotics in soil using continuous solvent extraction-solid phase extraction-liquid chromatography-tandem mass spectrometry. The method first uses continuous solvent extraction to extract the bioactive and extractable antibiotic components from the soil. Then, a solid phase extraction column is used for sample purification. Finally, multiple reaction monitoring (MRM) mode of liquid chromatography-tandem mass spectrometry is used to determine the concentrations of various antibiotics, including chlortetracycline, doxycycline, oxytetracycline, tetracycline, sulfadiazine, sulfadiazine, sulfamethoxazole, ciprofloxacin, enrofloxacin, norfloxacin, ofloxacin, azithromycin, erythromycin, roxithromycin, tylosin, trimethoprim, and lincomycin. This invention combines continuous solvent extraction with solid phase extraction, coupled with liquid chromatography-tandem mass spectrometry detection, to achieve accurate evaluation of trace antibiotic bioavailability under complex matrix conditions.

The invention discloses a dual-emission molecular imprinting ratiometric fluorescence sensor, a preparation method thereof and application of the dual-emission molecular imprinting ratiometric fluorescence sensor in aureomycin detection, and belongs to the technical field of chemical sensors. The preparation method of the sensor comprises the following steps: preparing silicon dioxide-quantum dot composite nanoparticles, and preparing a molecularly imprinted polymer by taking salicylamide as a template molecule; and mixing the molecularly imprinted polymer and the silicon dioxide-quantum dot composite nanoparticles in an HEPES buffer solution by adopting a post-mixing strategy to construct the dual-emission molecularly imprinted ratiometric fluorescence sensor. The double-emission molecular imprinting ratiometric fluorescence sensor has the beneficial effects that the double-emission molecular imprinting ratiometric fluorescence sensor provided by the invention is rich in fluorescence color change, rapid, high-sensitivity and visual detection of aureomycin can be realized, the whole detection process is simple, convenient and rapid, and the double-emission molecular imprinting ratiometric fluorescence sensor is suitable for being widely popularized in practical application.

The present application belongs to the technical field of microbial fermentation, and particularly relates to a fermentation method of aureomycin and application thereof. The present application reduces the mass of slow-acting nitrogen substances in fermentation raw materials, and adds papain in the fermentation raw materials, thereby improving the utilization rate of nitrogen substances in the fermentation raw materials to meet the fermentation demand of streptomyces aureofaciens, and reducing the use amount of nitrogen substances; meanwhile, the residual sugar concentration in the fermentation broth is reduced, thereby achieving the effect of reducing the COD of wastewater discharged in the front end of aureomycin fermentation. Meanwhile, the fermentation method is simple to operate and easy to popularize, which greatly saves the raw material cost and environmental protection cost, and greatly reduces the preparation cost of aureomycin.

The invention belongs to the technical field of veterinary drug residue detection, and particularly relates to a functionalized bentonite composite material for extracting and purifying veterinary drug residues in food as well as a preparation method, a kit and application of the functionalized bentonite composite material. Ferric nitrate nonahydrate modified bentonite is taken as a core, and the interlayer spacing is expanded from 1.25 nm to 1.53 nm through accurate intercalation regulation and control; an iron ion coordination-electrostatic adsorption difunctional synergistic mechanism is constructed, the tetracycline adsorption capacity is 39.78 mg / g, and the aureomycin removal rate is 90.35%.

The application discloses a Pandora strain capable of efficiently degrading aureomycin and an application thereof. The strain is FJNUIM-SW2, which is classified and named as Pandora (Pandoraea sp.) and is preserved in the China General Microbiological Culture Collection Center (CGMCC) on October 17, 2024, with a preservation number of CGMCC NO.32242 and a preservation address of No.3, Institute of Microbiology, Chinese Academy of Sciences, Beijing City, Chaoyang District, Beichen West Road 1st Courtyard 3rd. Pandoraea pnomenusa The strain FJNUIM-SW2 can grow by taking aureomycin as the only carbon source and energy source, has good aureomycin degradation characteristics, can be used for microbial degradation of aureomycin in aureomycin wastewater and soil and the like, provides a microbial resource for biological remediation of aureomycin in the environment, and has potential application value in ecological environment protection.

The application discloses a nucleic acid aptamer TET-HeR of tetracycline and an application thereof, and belongs to the technical field of nucleic acid aptamer. The nucleic acid aptamer TET-HeR of tetracycline is a combination of an aptamer TET-A1 and an aptamer TET-C1, the nucleotide sequence of the aptamer TET-A1 is shown as SEQ ID NO. 5, and the nucleotide sequence of the aptamer TET-C1 is shown as SEQ ID NO. 7. The nucleic acid aptamer TET-HeR of tetracycline is applied to the detection of tetracycline. The nucleic acid aptamer TET-HeR of tetracycline has an affinity dissociation constant of 62.7 nM with tetracycline, has high affinity, has no obvious affinity with aureomycin and doxycycline, has weak affinity with terramycin, has good specificity, and can be used for the specific detection of tetracycline.

The invention provides a preparation method of an N, S-CDs composite infinite coordination polymer and application of the N, S-CDs composite infinite coordination polymer in detection of aureomycin, oxytetracycline, metacycline, tetracycline and doxycycline. According to the N, S-CDs composite infinite coordination polymer used in the invention, 2-chloro-1, 4-phenylenediamine and thiourea are respectively used for providing an N source and an S source to synthesize green light-emitting carbon quantum dots as an object, Eu is used as a central ion, GMP is used as a ligand, and the N, S-doped carbon quantum dot object functionalized lanthanide series metal infinite coordination polymer fluorescent probe is constructed. On the basis of chemical structure analysis, it is fully determined that when tetracycline antibiotics exist, the antenna effect of Eu-GMP enables red fluorescence of the probe to be excited out, due to the inner filter effect, green fluorescence of the carbon dots is quenched, fluorescence signals of subjects and guests of G-CDs (at) Eu-GMP ICPs are adjusted by adding tetracycline antibiotics of different concentrations, and the fluorescence of the subjects and guests of G-CDs (at) Eu-GMP ICPs is detected. High-sensitivity detection of tetracycline antibiotics in animal-derived food can be realized, and food safety and environmental monitoring are facilitated.

The invention discloses a tetracycline hapten, a tetracycline antigen, a tetracycline antibody, a tetracycline detection device, preparation, application and used equipment, and relates to a tetracycline hapten, a tetracycline antigen, a tetracycline antibody, a tetracycline colloidal gold test strip, preparation of the tetracycline hapten, the tetracycline antigen, the tetracycline antibody and the tetracycline colloidal gold test strip, application of the tetracycline colloidal gold test strip in detection of tetracycline drug residues in a water sample and used equipment in preparation The invention provides a tetracycline hapten, the tetracycline hapten is a universal hapten designed and synthesized aiming at a general structure of a tetracycline drug, the hapten is applied to prepare an artificial antigen and an antibody for detecting the tetracycline drug, and the antibody has the characteristics of strong specificity and high sensitivity on the tetracycline drug. The tetracycline antibiotics in the water sample can be accurately detected, and the rapid detection of oxytetracycline, aureomycin and tetracycline as low as 5 [mu] g / L and the rapid detection of doxycycline and minocycline as low as 10 [mu] g / L in the water sample can be realized.

The invention discloses a preparation method of an antibody for detecting ovalbumin and vancomycin in a biological product, the ovalbumin antibody is obtained by directly immunizing an animal, the vancomycin antibody is prepared by carrying out nucleophilic substitution reaction on vancomycin and N-(P-maleimidophenyl) isocyanate to obtain a vancomycin hapten, and the vancomycin hapten is used for detecting the ovalbumin and vancomycin in the biological product. The vancomycin immunogen is obtained by coupling the vancomycin with carrier protein, and then animals are immunized to obtain the vancomycin immunogen. The antibody is prepared into an enzyme linked immunosorbent assay kit, and when the enzyme linked immunosorbent assay kit is used for detecting ovalbumin contained in a biological product, the detection limit is 200 g / L; when the kit is used for detecting vancomycin contained in a biological product, the detection limit is 0.5 g / L. The detection result is high in accuracy, especially in a vancomycin antibody specificity experiment, the detection result shows that the detection result has remarkable specificity on vancomycin, and the detection result is not interfered by drugs such as polymyxin E, kanamycin, streptomycin, gentamicin, penicillin, tetracycline, chloramphenicol, neomycin, aureomycin, oxytetracycline and doxycycline.