87 results about "Aureomycine" patented technology

The invention belongs to the technical field of aureomycin preparation, and particularly relates to an aureomycin fermentation culture medium and an aureomycin fermentation production method by using the same. Every 1L of culture medium is composed of 12-22g of peanut meal, 8-20g of soybean cake powder, 50-100g of corn starch, 10-14g of corn steep liquor, 5.0-9.0g of yeast powder, 2.5-5g of bean oil, 0.1-0.2g of amylase, 1.5-3.5g of sodium chloride, 5-8g of ammonium sulfate, 5.6-8.0g of calcium carbonate, 0.1-0.3g of magnesium sulfate and 0.1-0.5g of potassium dihydrogen phosphate. The pH value is natural. The invention provides an optimized aureomycin fermentation culture medium. The sesame meal is partially or completely substituted for the soybean cake powder for further optimization, thereby obtaining favorable fermentation effects.

The invention discloses a formula of veterinary chlortetracycline hydrochloride soluble powder and a preparation method thereof. The soluble powder is prepared from the following components in percentage by weight: 10-50 percent of chlortetracycline hydrochloride, 0.2-3.0 percent of sodium dodecyl sulfate, 0.1-1.0 percent of ethylenediamine tetraacetic acid disodium salt and the balance of glucosum anhydricum. The chlortetracycline hydrochloride is utilized as a raw material, sodium dodecyl sulfate is added as an absorption enhancer, and ethylenediamine tetraacetic acid disodium salt is utilized as an antioxidant and a chelating agent, so that the stability and high efficiency of chlortetracycline hydrochloride soluble powder are ensured.

The invention relates to a screening method of aureomycin degrading strains, and belongs to the technical field of degrading antibiotic residues by microorganisms. The screening method comprises the following particular steps: isolating the aureomycin degrading strains; purifying the strains; verifying the degrading functions of the strains; and determining the degrading activities of the strains. The screening method adopted by the invention is quick, feasible, economic and low-priced, and is suitable to obtain strains capable of degrading aureomycin residues of different concentrations, especially the efficient strains capable of degrading low-concentration or oligotrophic-grade aureomycin. The obtained strains can be used for preparing an aureomycin degrading agent. According to the invention, the aureomycin-producing microbe culture residue and waste water are utilized as the original materials for screening; and the obtained strains and the prepared agents can be directly applied in treating waste of aureomycin production enterprises, and can be possibly applied in remediation of peripheral soil and water environment polluted by aureomycin. According to the invention, high performance liquid chromatography is used for determining the degrading activities of the strains, and the obtained results have high accuracy and precision.

The invention belongs to the technical field of the application of feed additives, and particularly relates to a novel application of a nano compound as a feed additive. The invention provides a novel application of the trehalose-montmorillonite nano compound as the feed additive, and preferably 1 to 2g/kg of the nano compound is added in the feed. The trehalose-montmorillonite nano compound has a positive effect on killing harmful intestinal bacteria, protecting intestinal mucosa and promoting the growth of animals and can substitute the conventional antibiotic-aureomycin of the feed additive. The trehalose-montmorillonite nano compound has the characteristics of safety and environmental friendliness. Through the application of the trehalose-montmorillonite nano compound, the defect that the antibiotics are widely applied in the breeding industry can be effectively overcome, and a safe animal product conforming to the internal standard can be produced.

The invention discloses a method for rapidly determining contents of aureomycin for feed and impurities of aureomycin by HPLC. The method includes the following steps: preparing reagents and solutions, preparing chromatographic conditions, carrying out operational suitability test of HPLC system, and analyzing chromatograms. The method disclosed by the invention adopts HPLC precision detection instrument for determination, can determine contents of aureomycin for feed and chlorquatrimycin and tetracycline impurities simultaneously, and has the advantages of accurate determination results, rapid determination, and high determination precision.

The invention relates to swine-origin lactobacillus reuteri and prepared solid probiotics. The bacterial strain is separated from fresh night soil of healthy live pig, and is indentified as Lactobacillus reuteri YSJL-12 through a morphological biochemistry and molecular biology method, and a preservation number is CGMCC No. 9062. The bacterial strain inhibits Listeria monocytogenes CICC21633 to positive. The prepared solid probiotics contain soybean meal and active Lactobacillus reuteri YSJL-12, can substitute aureomycin for preventing and treating diarrhoea while added in a live pig basic feed, and the feed-gain ratio of the breeding pork pig at age in days of 28-168 is 2.61. The bacterial strain has the characteristics of strong host specificity, large live bacteria quantity, low cost and high cost performance, added value of high temperature soybean meal is increased, and the green live pig culture cost is reduced.

The invention discloses a veterinary-use intestinal targeted antibacterial pellet. The veterinary-use intestinal targeted antibacterial pellet comprises a main drug and auxiliary drugs, wherein the main drug is composed of valnemulin hydrochloride and chlortetracycline hydrochloride; the mass of the valnemulin hydrochloride is 1-5% of that of the pellet; the mass of the chlortetracycline hydrochloride is 5-16 times of that of the valnemulin hydrochloride; the pellet sequentially comprises a valnemulin hydrochloride core, an intestinal targeted coating layer, a chlortetracycline hydrochloride drug layer and an outer coating layer from inside to outside. The invention further discloses a preparation method of the veterinary-use intestinal targeted antibacterial pellet. Through the combination of the method and the formula, the valnemulin hydrochloride and the chlortetracycline hydrochloride are adopted for preparing the pellet; the pellet is released in a gradient form in an animal digestion system; the chlortetracycline hydrochloride is firstly released and absorbed by the stomach and the intestine; the valnemulin hydrochloride is rapidly released and absorbed by the intestinal tract with the pH value larger than or equal to 6.8, so that an excellent intestinal tract sterilization effect is achieved, the drug absorbed by the colon can be prevented from suffering from the first-pass effect of the liver and the intestine, the haemoconcentration and bioavailability of the valnemulin hydrochloride can be improved, and an excellent treatment effect for systemic diseases is obtained.

The invention relates to the field of environment protection, and discloses a tetracycline antibiotics degrading arthrobacterium and application thereof. The classification name of the tetracycline antibiotics degrading arthrobacterium is Arthrobacter sp.. The Arthrobacter sp. is tetracycline antibiotics degrading arthrobacterium, can effectively degrade various tetracycline antibiotics, has the best oxytetracycline degrading effect, and has the good degrading effect for tetracycline and aureomycin. The bacterial strain OTC-16 can grow under the pH of 6.0-9.0 and under the temperature of 20-35DEG C, the application range is wide, and the tetracycline antibiotics degrading arthrobacterium can be used for repairing tetracycline antibiotics pollution.

The present invention discloses an ELIA kit applicable to aureomycin recidue analysis and its application. Said kit includes kit body, enzyme-labelled plate and reagent, in which said reagent includes washing solution, sample diluent, horseradish peroxidase labeled antibody, substrate solution, color developing solution and stop buffer. The invented kernel lies in that it has the coated antigen, which is coated by coating solution and can be specifically reacted with aureomycin antibody, aureomycin standard solution and aureomycin antibody. Said antibody is the blood serum that is obtained by immunizing rabbit by utilizing artificial immune source, the coated antigen is the compound formed from aureomycin acetic acid and egg-white albumin, and the artificial immune source is the compound composed of aureomycin acetic acid and bovine serum albumin.

The invention relates to an indicator strain with a classification name of anoxybacillussp. FHB009. The indicator strain is preserved at China Center for Type Culture Collection, and has a preservation number of CCTCC NO: M2013128. For prolonging the service life of a kit, the spores of the strain are adopted. A culture medium embedding the spores is a modified beef extract peptone medium with a pH value of 5-10. The indicator strain provided by the invention can sensitively detect various commonly used antibiotics such as sulfadiazine, sulfamethazine, oxytetracycline, tetracycline, chlortetracycline, gentamicin, doxycycline, penicillin, ampicillin, ampicillin, and the like, wherein a sensitivity limit satisfies an antibiotic highest residue standard of national standard (GB/T4789.27-2008).

The invention relates to preparation of a tetracycline antibiotic (tetracycline, oxytetracycline, aureomycin and doxycycline) multi-template molecularly imprinted magnetic composite material and application of the composite material in removal and detection of tetracycline antibiotics. After the surface of magnetic graphene oxide is coated with mesoporous silica, tetracycline, oxytetracycline, aureomycin and doxycycline are jointly used as template molecules, anilinomethyltriethoxysilane and N-[3-(trimethoxysilyl) propyl] ethylenediamine are jointly used as functional monomers, and a sol-gel method is adopted for surface molecular imprinting. The obtained tetracycline antibiotic multi-template molecularly imprinted magnetic composite material can be used for carrying out high-selectivity adsorption on tetracycline, oxytetracycline, aureomycin and doxycycline in a solution at the same time; the adsorption capacities are 5.9 mg/g, 5.7 mg/g, 19.5 mg/g and 11.9 mg/g respectively, adsorption equilibrium is achieved within 60 min, the adsorbent can be repeatedly used five times or more, and the material can be used for removing and detecting tetracycline antibiotics.

The invention discloses a compound premix for Nongda No. 3 grain-saving small laying hens in the brooding period. The compound premix comprises the following components in parts by mass: 27 to 33 DL-methionine, 18 to 22 lysine sulphate, 5.5 to 6.55 choline chloride, 1.125 to 1.245 aureomycin, 2.6 to 3.3 phytase, 0.4 to 0.6 antioxidant, 370 to 450 rock flour, 80 to 90 common salt, 230 to 270 calcium hydrophosphate, 221.5 to 77.7 medical stone, 25 compound trace elements for Nongda No.3 grain-saving small laying hen chicklings and 7 multivitamin for Nongda No.3 grain-saving small laying hen chicklings. The premix is specially developed according to physiological characteristics and nutritional needs of the Nongda No. 3 grain-saving small laying hens, has strong pointedness, well meets the nutritional needs of the Nongda No. 3 grain-saving small laying hen chicklings, and has the advantages of obvious feeding effect, high survival rate of the chicklings, high rate of compliance of weightand good uniformity. The invention has great economic value to cultivation of the Nongda No. 3 grain-saving small laying hens in the laying period.

The invention relates to the field of microbial preparations, in particular to a biodegradable complex microbial inoculant for aureomycin fungus residue waste and a compost high-temperature pre-fermentation method of the biodegradable complex microbial inoculant for the aureomycin fungus residue waste. The biodegradable complex microbial inoculant for the aureomycin fungus residue waste comprises bacillus natto powder and aspergillus niger powder, bacillus tequilariae powder and/or phanerochaete chrysosporium powder are compounded, and the total viable count of the complex microbial inoculant is greater than or equal to 1 * 10 <8> cfu/g. According to the complex microbial inoculant, bacillus capable of tolerating aureomycin and mould are selected to form the complex microbial inoculant, aureomycin fungus residue waste added with a certain amount of bran is fermented, and the temperature of the fermentation process is rapidly increased through the synergistic effect of several strains capable of degrading aureomycin and decomposing protein, amino acid and a large number of carbon sources in thalli; the degradation of the aureomycin is accelerated. The fungus residues with lower aureomycin concentration are also beneficial to further growth and reproduction of the strains, and the synergistic fermentation is rapidly carried out, so that the fermentation and decomposition time is shortened, and the decomposition is more complete, thereby realizing resource utilization.

The invention belongs to the technical field of fermentation culture, and particularly relates to a fermentation medium for producing demethylated aureomycin, the fermentation medium contains a carbonsource, a nitrogen source and a phosphorus source, molasses is also added as a source for providing biotin and pantothenic acid, and calcium chloride is also added to replace sodium chloride as an inorganic salt source. According to the method, the titer of demethylated aureomycin can be increased, the content of impurity demethylated tetracycline can be reduced, and the method is efficient, simple and low in cost.

The invention relates to a preparation method of hydrochloric acid aureomycin; the technical steps are listed below: firstly acidifying aureomycin broth; adjusting pH to 2-2.5; standing; continuously adjusting pH to 4-4.5; standing; adding water to reduce viscosity to 3000-4000cps pretreatment; carrying out membrane filtration, nanofiltration, crystallization, washing, salt forming, leaching and drying to obtain hydrochloric acid aureomycin powders. The method can improve extraction yield of the hydrochloric acid aureomycin, reduces production cycle and cost, and improves hydrochloric acid aureomycin output.

The invention discloses a preparation method of a casein-gold nanocluster and application of the casein-gold nanocluster in aureomycin detection. The preparation method comprises the following specific steps of S1, weighing a certain amount of casein, adding 750 microliters of water for dissolution, then adding a sodium hydroxide solution for adjusting the pH of the solution, and conducting heating for 15-20 minutes in a water bath at the temperature of 37-90 DEG C, so as to obtain a solution A; S2, adding chloroauric acid into the solution A obtained in the step S1, and conducting heating in a water bath at 37-90 DEG C for 0-5 hours to obtain a solution B; and S3, storing the solution B obtained in the step 2 under the condition of 4 DEG C to obtain the casein-gold nanocluster. The fluorescent gold nano-cluster has unique photophysical characteristics, is simple to prepare, almost non-toxic and excellent in biocompatibility, the aureomycin detection method is rapid and simple, the detection sensitivity is high, the detection limit is low, and the fluorescent gold nano-cluster is an ideal fluorescent nano-material applied to the fields of biology and medicine.

The invention discloses a method for producing aureomycin by using streptomyces aureus. According to the method, a culture medium and a culture method are combined and optimized, so that the synthesis and accumulation of aureomycin are promoted, and the proportion of impurity tetracycline and demethyl aureomycin is further reduced. A high-yield strain breeding model is constructed based on multiple composite mutagenesis and precursor/product tolerance, so that the diversity of a mutation library is higher, the method is simple, convenient and rapid, the screening efficiency is improved, the screening workload is reduced, and the method has certain application value and guiding significance for the reduction of the production cost and the improvement of the productivity of aureomycin andother streptomyces-derived antibiotics. The mutant strain can efficiently synthesize and accumulate aureomycin, the content of the impurity tetracycline and demethyl aureomycin is remarkably reduced, the genetic stability is good, the form, color, aureomycin production level and the content of main impurities are basically stable after continuous passage for five generations, and the mutant strain can be applied to industrial fermentation production and can be used as a production strain for further research and development.

The invention discloses a streptomyces aureus mutant strain, a breeding method thereof and an optimized culture medium. The mutant strain can efficiently synthesize and accumulate aureomycin, the content of impurity tetracycline and demeclocycline is remarkably reduced, the genetic stability is good, the form, color, aureomycin production level and main impurity content of the mutant strain are basically stable after five generations of continuous passage, and the mutant strain can be applied to industrial fermentation production and can be used as a production strain for further research and development. The optimized culture medium is beneficial to promoting synthesis and accumulation of the aureomycin, and the proportion of the impurity tetracycline and demeclocycline is further reduced. A high-yield strain breeding model is constructed based on multiple compound mutagenesis and precursor/product tolerance, the diversity of a mutation library is higher, simpleness, convenience and quickness are achieved, the screening efficiency is improved, the screening workload is reduced, and certain application value and guiding significance for reducing the production cost and improving the productivity of the aureomycin and other streptomycete source antibiotics are achieved.