Compositions and methods for in vivo post translational modification

A post-translational modification and composition technology, applied in biochemical equipment and methods, chemical equipment and methods, antibody mimics/scaffolds, etc., can solve problems such as reducing the functionality of biological products

Pending Publication Date: 2021-03-30
THE WISTAR INST OF ANATOMY & BIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Biologics currently used as drugs (antibodies, erythropoietin, coagulation factors) are often produced in mammalian cell lines (CH

Method used

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  • Compositions and methods for in vivo post translational modification
  • Compositions and methods for in vivo post translational modification
  • Compositions and methods for in vivo post translational modification

Examples

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example 1

[0332] Example 1: Synthetic DNA Delivery by Electroporation Promotes Robust In Vivo Sulfation of the Broadly Neutralizing Anti-HIV Immunoadhesin ECD4-IG

[0333] Transfection of HEK293T cells enables expression and secretion of ReCD4-Ig in vitro

[0334] A transgene encoding ReCD4-Ig with an N-terminal IgGκ-leader sequence was designed, then synthesized de novo, and cloned into the pGX00001 backbone plasmid. Transgene nucleotide sequences were optimized for codon bias and structure and stability of mRNA transcripts in both mice and humans (Graf et al., 2004; Patel et al., 2017). An N-terminal IgG leader sequence is incorporated to facilitate targeting of the transgene to the ER and facilitate secretion (Haryadi et al., 2015). Strong expression of ReCD4-Ig was observed in cell lysates and supernatants (Fig. 1A-B). Immunoblot of transfection supernatants with anti-human IgG confirmed secretion of ReCD4-Ig by transfected cells (Fig. 1C).

[0335] Co-transfection of HEK293T...

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Abstract

The present invention provides methods of post-translationally modifying a synthetic protein in a subject. In one embodiment, the method comprises administering to the subject a composition comprisinga first recombinant nucleic acid sequence encoding the synthetic protein, and a second recombinant nucleic acid sequence encoding a modifier protein, wherein the modifier protein post-translationallymodifies the synthetic biologic in the subject. In one embodiment, the post translational modification is sulfation and the modifier protein is selected from the group consisting of tyrosylprotein sulfotransferase 1 (TPST1) and TPST2.

Description

[0001] Cross References to Related Applications [0002] This application benefits from US Provisional Application No. 62 / 683,344, filed June 11, 2018, which is incorporated herein by reference in its entirety. Background technique [0003] The activity of various proteins can be enhanced through post-translational modifications. Post-translational modifications (PTMs) are chemical changes that result in the covalent attachment of different functional groups to proteins. These modifications can specifically target the protein to certain cellular pathways, improve overall function and potency, alter stability or half-life, or result in differences in folding and other protein interactions. The ability to encode different target proteins is well established using DNA plasmids. However, the need to further improve these DNA-encoded proteins is warranted. By encoding different enzymes that can perform post-translational modifications, the target protein can be modified and thus...

Claims

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Application Information

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IPC IPC(8): A61K47/68C12N9/10
CPCC12N9/10A61K41/0047C12N9/13C07K14/70514C07K2319/30C07K14/705C12N15/62C12Y208/0202A61K38/1774C07K2317/52C07K2317/40C07K2319/32A61K38/45
Inventor 大卫·韦纳梅甘·怀斯徐子扬
Owner THE WISTAR INST OF ANATOMY & BIOLOGY
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