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Tumor tissue preserving fluid and application

A technology of tumor tissue and preservation solution, applied in the biological field, can solve the problems of limited nutrient content of DMEM medium, inability to meet the storage and transportation of tumor tissue, and difficulty in playing a buffer role, etc., to reduce cell damage, small batch-to-batch difference, and high success rate high effect

Active Publication Date: 2021-04-02
GUANGZHOU DARUI BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there may be certain deficiencies under longer-term preservation and transportation conditions: (1) The sodium bicarbonate buffer system is used in the preservation solution. Under normal conditions, CO 2 The concentration is low, and it is difficult to exert a good buffering effect; (2) DMEM medium has limited nutritional components, lacks certain lipids and metal ion carrier proteins, and cannot meet the needs of longer storage and transportation of tumor tissues; (3) Lack of precautions to prevent cell edema that may cause damage to cells

Method used

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  • Tumor tissue preserving fluid and application
  • Tumor tissue preserving fluid and application
  • Tumor tissue preserving fluid and application

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Experimental program
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Effect test

Embodiment 1

[0055] Embodiment 1 A kind of tumor tissue preservation solution

[0056] Composition: basal medium Leibovitz's L-15; BSA, 2% (w / v); recombinant human insulin, 10 μg / ml; transferrin, 5 μg / ml; linoleic acid, 5 μg / ml; Trace Elements A (1000× ), 0.1% (v / v); Glutamax (100×), 1% (v / v); Trehalose, 2mg / ml; γ-polyglutamic acid (500kDa), 0.075% (w / v); Primocin, 100 μg / ml.

Embodiment 2

[0057] Embodiment 2 A kind of tumor tissue preservation solution

[0058] Composition: basal medium Leibovitz's L-15; BSA, 2% (w / v); recombinant human insulin, 1 μg / ml; transferrin, 5 μg / ml; linoleic acid, 5 μg / ml; Trace Elements A (1000× ), 0.1% (v / v); Glutamax (100×), 1% (v / v); Trehalose, 2mg / ml; γ-polyglutamic acid (500kDa), 0.075% (w / v); Primocin, 100 μg / ml.

Embodiment 3

[0059] Embodiment 3 A kind of tumor tissue preservation solution

[0060] Composition: basal medium Leibovitz's L-15; BSA, 2% (w / v); recombinant human insulin, 30 μg / ml; transferrin, 5 μg / ml; linoleic acid, 5 μg / ml; Trace Elements A (1000× ), 0.1% (v / v); Glutamax (100×), 1% (v / v); Trehalose, 2mg / ml; γ-polyglutamic acid (500kDa), 0.075% (w / v); Primocin, 100 μg / ml.

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Abstract

The invention discloses a tumor tissue preserving fluid and application. The primary tumor tissue preserving fluid does not contain serum, and is clear in components and small in inter-batch difference. BSA, trehalose, gamma polyglutamic acid, linoleic acid and other components are used in a combined mode, synergistic interaction is achieved, cell acidosis, edema and external physical stress caused by long-time transportation and preservation conditions can be effectively overcome, cell damage is reduced, the tissue morphological structure and cell viability are maintained, and along with prolonging of the preservation time, the cell viability of cancer tissues is slowed down, the success rate of embedding culture of preserved cell glue drops is high, and the requirement of longer-time preservation and transportation of tumor tissues can be met.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a tumor tissue preservation solution and its application. Background technique [0002] With the development of regenerative medicine and stem cell research, the value of scientific research and clinical application of living tumor tissue has become increasingly prominent. The collection and preservation of clinical fresh tumor tissue specimens after ex vivo is crucial to the quality of the specimens, which directly affects the reliability of later research. During the collection and transportation of tissue samples, choosing an appropriate storage medium can maintain the tissue structure and cell activity. Conversely, improper storage may lead to tissue cell apoptosis. [0003] Usually, the tumor tissue is stored in an appropriate preservation solution at a low temperature of 2-8°C for transportation to reduce the enzyme activity in the cell, thereby reducing the metabolic rate of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0226
Inventor 万勇徐中华李明
Owner GUANGZHOU DARUI BIOTECH
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