The invention discloses a Chinese herbal medicine feed additive for improving the beef quality and a preparation method of the Chinese herbal medicine feed additive. The Chinese herbal medicine feed additive comprises, in parts by weight, 36-70 parts of refined traditional Chinese medicine powder, 0.2-0.7 part of a flavoring agent, 0.38-0.56 part of microelements and 4-8 parts of a meat quality regulating agent; the refined traditional Chinese medicine powder comprises raw materials as follows: all-grass of delavay pararuellia, thinleaf milkwort root-bark, vitis flexuosa, cowpea roots, cistanche, medicinal fermented mass, microcos leaves, poria cocos, atractylodes lancea, chicken's gizzard-membrane, fructus amomi, liquorice, almonds, mulberry leaves, areca nuts, fructus aurantii, officinal magnolia bark, peach seeds, fructus cannabis and immature bitter oranges. The Chinese herbal medicine formula has effects of moistening five internal organs, promoting muscles to grow, strengthening muscles and bones, regulating qi, strengthening the spleen and invigorating the stomach, the special meat quality regulating agent can effectively ensure that a large number of cereals are eaten in a fattening period, the deposition of fat among muscle fibers is increased, meanwhile, the pH in the stomach is regulated, acidosis is prevented, the normal growth of microorganisms in the stomach is guaranteed, and purposes of fattening and the improvement on the beef quality are achieved.