Preparation method of special bacterium enzyme compound feed additive for ruminants

A compound additive and ruminant technology, applied in animal feed, animal feed, microbial-based methods, etc., can solve the problems of probiotics inactivation, failure to function, and easy mildew

Inactive Publication Date: 2021-09-10
内蒙古柯宏生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Previous studies have found that lactic acid bacteria produce lactic acid during the fermentation process, which causes a decrease in rumen pH after being eaten by ruminants, leading to rumen acidosis. Therefore, lactic acid bacteria are not conducive to the rumen microecological balance of ruminants
However, the pH of products without lactic acid bacteria will be relatively high, resulting in a short shelf life, easy to mildew and agglomeration, and there is no good solution at present
[0004] (2) Traditional feed com

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1: specific ruminant animal lysozyme prepared composite feed additive, which comprises the following steps: (1) screen a Bacillus subtilis and Saccharomyces cerevisiae; (2) a liquid fermentation of Bacillus subtilis bacteria; ( 3) to obtain a liquid fermentation broth of S. cerevisiae; (4) proportionally complex enzyme preparation; (5) preparation of solid medium; (6) prepared by solid state fermentation in ruminants dedicated lysozyme finished composite additive; wherein,

[0030] (1) by conventional methods in the art were screened Bacillus subtilis and Saccharomyces cerevisiae;

[0031] (2) a liquid fermentation of Bacillus subtilis bacteria: Bacillus subtilis proportion to the preparation liquid medium, and then screened Bacillus subtilis Bacillus subtilis inoculated liquid medium for fed-batch fermentation, Bacillus prepared Bacillus bacteria, Bacillus subtilis bacteria, the viable cell count is 1.0 * 10 ^ 10CFU / mL;

[0032] In this embodiment, the liquid med...

Embodiment 2

[0045] Example 2: An animal lysozyme complex special preparation ruminant feed additive, Example 1 is different from the aqueous medium mass percentage of solid content of 25%, the remaining steps and parameters identical to Example 1. Bacteria present embodiment ruminants specific enzyme complex obtained in Preparation Example viable cell count in the finished additive is 2.3 * 10CFU / mL.

Embodiment 3

[0046] Example 3: An animal lysozyme complex special preparation ruminant feed additive, Example 1 is different from the solid aqueous medium mass percentage of 35%, the remaining steps and parameters identical to Example 1. Bacteria present embodiment ruminants specific enzyme complex obtained in Preparation Example viable cell count in the finished additive is 6.3 * 10CFU / mL.

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Abstract

The invention discloses a preparation method of a special bacterium enzyme compound feed additive for ruminants. The preparation method comprises the following steps of (1) screening bacillus subtilis and saccharomyces cerevisiae separately; (2) performing liquid fermentation to obtain a bacillus subtilis liquid; (3) performing liquid fermentation to obtain a saccharomyces cerevisiae liquid; (4) preparing a compound enzyme in proportion; (5) preparing a solid culture medium; and (6) performing solid-state fermentation to obtain the special bacterium enzyme composite additive for ruminants. According to the method, lactic acid bacteria and amylase are not added, the problems of rumen pH reduction and rumen acidosis caused by ingestion by ruminants are avoided, and meanwhile, on the premise of ensuring the viable count, rich enzyme systems are fused, and the particle size of the crushed solid culture medium is reasonably controlled, so that the product quality can still be ensured under the condition that lactic acid bacteria are not added, and the product is free of mildewing and caking after being stored for more than 12 months at normal temperature; and the rumen fermentation function of ruminants can be improved, the conversion of nutrient substances is promoted, and the production performance is enhanced.

Description

Technical field: [0001] The present invention relates to a method for preparing a composite feed additive, in particular, it relates to a method for preparing bacteria-specific enzyme complex ruminant feed additive. Background technique: [0002] Microbial feed additive is nearly 10 years as an alternative to antibiotics developed a class of prevention of gastrointestinal diseases, reducing mortality in young animals, improved feed efficiency, promoting growth of animals and so on, and good safety feed additives. At present, the traditional microbial feed additive following deficiencies: [0003] (1) microbial agents conventional forage additive is generally added: Bacillus subtilis, yeasts and lactic acid bacteria. After previous studies found that lactic acid bacteria produce lactic acid during the fermentation process, after the feeding of ruminants caused by rumen pH decreased, resulting in rumen acidosis, therefore, is not conducive to lactic acid bacteria rumen microflora b...

Claims

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Application Information

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IPC IPC(8): A23K50/10A23K10/12A23K10/14A23K10/30A23K10/37A23K10/33A23K10/18C12N1/20C12N1/18C12R1/125C12R1/865
CPCA23K50/10A23K10/12A23K10/14A23K10/30A23K10/37A23K10/33A23K10/18C12N1/20C12N1/18Y02P60/87
Inventor 祝琳敬高海涛贺慧霞
Owner 内蒙古柯宏生物科技有限公司
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