Microbial conditioner for activating soil selenium and preparation method thereof
A microbial agent and microbial technology, applied in the direction of fertilizer mixture, application, organic fertilizer, etc., can solve the problems of difficulty in meeting selenium intake requirements, low selenium content in soil, low selenium content, etc., and achieve significant economic and environmental benefits. Effect of improving soil structure and increasing selenium content
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Embodiment 1
[0023] A microbial conditioning agent for activating soil selenium is characterized in that it is made of the following raw materials by weight percentage: 50% of farmyard manure, 20% of limestone powder, 20% of potassium fulvic acid, and 10% of selenophilic microbial bacterial agent.
[0024] Prepare the microbial conditioner for activating soil selenium:
[0025] (1) Microbacterium oxydans YLX-2 strains were inserted into 1 mL of sterilized soybean trypsin medium, and cultured at a temperature of 30° C. and a rotation speed of 200 rpm for 8 hours.
[0026] (2) Pipette 250 μL of the bacterial solution obtained in the above (1) into 1000 mL of sterilized soybean trypsin medium, and continue to cultivate for 16 hours at a temperature of 30° C. and a rotational speed of 200 rpm to obtain a selenophilic microbial bacterial agent.
[0027] (3) Mix farmyard manure according to the weight ratio of pig manure and cow manure at 1:1, build a fermentation pile, and ferment for 5 days. ...
Embodiment 2
[0031] A microbial conditioning agent for activating soil selenium, which is composed of the following raw materials by weight percentage: 55% of farmyard manure, 15% of limestone powder, 20% of potassium fulvic acid and 10% of selenophilic microbial bacterial agent.
[0032] The finished product is made according to the following steps:
[0033] (1) Microbacterium oxydans YLX-2 was inserted into 1 mL of sterilized soybean trypsin medium, and cultured for 8 hours at a temperature of 30° C. and a rotational speed of 200 rpm.
[0034] (2) Pipette 250 μL of the bacterial solution obtained in the above steps into sterilized 1000 mL soybean trypsin medium, and continue culturing for 16 hours at a temperature of 30° C. and a rotational speed of 200 rpm to obtain a selenophilic bacterial agent.
[0035] (3) Mix farmyard manure according to the weight ratio of pig manure and cow manure at 1:1, build a fermentation pile, and ferment for 5 days.
[0036] (4) Mix the fermented farmyard ...
Embodiment 3
[0039] A microbial conditioner for activating soil selenium is prepared from the following raw materials in weight percentage: 60% of farmyard manure, 20% of limestone powder, 15% of potassium fulvic acid and 5% of selenophilic microbial bacterial agent.
[0040] The finished product is made according to the following steps:
[0041] (1) Microbacterium oxydans YLX-2 strains were inserted into 1 mL of sterilized soybean trypsin medium, and cultured at a temperature of 30° C. and a rotation speed of 200 rpm for 8 hours.
[0042] (2) Pipette 250 μL of the bacterial solution obtained in the above step (1) into 1000 mL of sterilized soybean trypsin medium, and continue culturing for 16 hours at a temperature of 30° C. and a rotational speed of 200 rpm to obtain a selenophilic bacterial agent.
[0043] (3) Mix farmyard manure according to the weight ratio of pig manure and cow manure at 1:1, build a fermentation pile, and ferment for 5 days.
[0044] (4) Mix the fermented farmyard ...
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