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Latex enhanced turbidimetric immunoassay kit for determining vancomycin

A technology of vancomycin and immune turbidimetry, which is applied in the direction of biological testing, measuring devices, material inspection products, etc., can solve the problems of high detection cost, cumbersome operation, and complicated operation, so as to ensure sensitivity, specificity, and good repeatability and accuracy, cheap effect

Pending Publication Date: 2021-04-13
SHANGHAI YUNZE BIOTECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among them, the HPLC-MS method is accurate and sensitive for the determination of blood drug concentration, but its operation is cumbersome, the detection time is long, the detection cost is high, and expensive equipment is required. It is mainly used in the field of scientific research or as a reference method
CMIA is a commonly used detection method at present. Its detection is highly automated and the detection results are accurate. However, the reagents are expensive and special matching instruments are required.
The enzyme-linked immunosorbent assay has the disadvantages of long detection time, complicated operation, and poor repeatability.

Method used

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  • Latex enhanced turbidimetric immunoassay kit for determining vancomycin
  • Latex enhanced turbidimetric immunoassay kit for determining vancomycin
  • Latex enhanced turbidimetric immunoassay kit for determining vancomycin

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Embodiment Construction

[0041] In order to make the purpose, technical solution and advantages of the present invention clearer, the technical solution of the present invention will be further described below.

[0042] The present invention proposes a latex-enhanced immune turbidimetric kit for determining vancomycin, including reagent R1, reagent R2 and vancomycin calibrator;

[0043] Reagent R1 includes 400-900ug / L vancomycin detection antigen, 5.33g / L MES monohydrate, 9g / L NaCl, 5g / LBSA, 0.5g / LMgCl 2 , 5mL / L 10%NaN 3 solution, 5g / L EDTA-2K and 18g / L dextran (450000-600000);

[0044]Reagent R2 includes vancomycin antibody, 0.8-1.4mL / L vancomycin sensitized latex microspheres, 11.26g / LMOPSO, 5g / LNaCl, 0.5% Blockmaster DB1130, 5mL / L 10% NaN 3 Solution, 10g / L sucrose, 10g / L trehalose and 18g / L dextran.

[0045] The pH of reagents R1 and R2 is 5.0-9.0; the concentration of buffer is preferably 15-100 mM.

[0046] In this embodiment, the preparation method of vancomycin detection antigen is as follo...

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Abstract

The invention provides a latex enhanced turbidimetric immunoassay kit for determining vancomycin. The latex enhanced turbidimetric immunoassay kit comprises a reagent R1, a reagent R2 and a vancomycin calibrator. The reagent R1 comprises a vancomycin detection antigen, a buffer agent, NaCl, BSA, MgCl2, a 10% NaN3 solution, EDTA-2K and glucan. The reagent R2 is prepared from a vancomycin antibody, vancomycin sensitized latex microspheres, a buffer agent, NaCl, Blockmaster DB1130, a 10% NaN3 solution, cane sugar, trehalose and glucan. Therefore, the vancomycin detection kit has the characteristics of simplicity and quickness in operation and high sensitivity, has good accuracy and stability, and can be highly consistent with a gold standard HPLC detection value.

Description

technical field [0001] The invention relates to the technical field of vancomycin detection, in particular to a latex-enhanced immune turbidimetric kit for determining vancomycin. Background technique [0002] Vancomycin, a polypeptide antibiotic, usually exists in the form of hydrochloride, which can be precipitated by various heavy metal salts in solution. As a narrow-spectrum antibiotic, it is only effective against Gram-positive bacteria, such as hemolytic streptococcus, pneumococcus and enterococcus, which are sensitive to drug-resistant Staphylococcus aureus. Its mechanism of action is to inhibit the synthesis of bacterial cell walls. It is mainly combined with bacterial cell walls, so that certain amino acids cannot enter the glycopeptides of the cell walls. It is generally used as the "last line of defense" for antibiotics. Vancomycin is the treatment for methicillin-resistant Severe infections caused by Staphylococcus aureus, methicillin-resistant coagulase-negativ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/531G01N33/543G01N33/68
CPCG01N33/531G01N33/54313G01N33/9446G01N33/6854
Inventor 刘斌虎吴冯波
Owner SHANGHAI YUNZE BIOTECH
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