Linker primer combination for library construction and kit thereof

Abstract

The invention discloses a linker primer combination for library construction and a kit thereof. The linker primer combination comprises a first linker, a second linker, a first primer and a second primer, head and tail parts of the first linker and the second linker contain nucleotide sequences that can be complementarily paired and linked, the middle part of the first linker contains a nucleotide sequence which cannot be connected to the second linker in a complementary pairing manner, the middle part of the second linker contains a nucleotide sequence which cannot be connected to the first linker in a complementary pairing manner, the first primer contains a nucleotide sequence which is the same as a nucleotide sequence which is not in complementary pairing connection with the middle part of the first linker and is connected to the second linker, both the first linker and the second linker do not contain a sample index, and the second primer contains a sample label and a nucleotide sequence which can be in partial complementary pairing connection with the second linker. According to the linker primer combination and the kit, the short joint sequence without an index is used, such that the connection efficiency is significantly improved, the synthesis cost is reduced, the amplification primers are lengthened, and the amplification efficiency is effectively improved.

Description

technical field [0001] The invention relates to the field of genomics, in particular to a linker primer combination and a kit for library construction. Background technique [0002] High-throughput sequencing technology (High-throughput sequencing), also known as "next-generation" sequencing technology ("Next-generation" sequencing technology) or "NGS" for short, can sequence hundreds of thousands to millions of DNA molecules in parallel at a time. Assays and generally shorter read lengths etc. are flagged. All current next-generation sequencing platforms need to construct a gene library before sequencing the DNA sequence, and this gene library needs to contain adapter sequences customized by extension or ligation. The linker is generally composed of several parts: library amplification sequence, index sequence, sequencing primer binding sequence, and molecular tag sequence. [0003] Existing linker ligation efficiency is low and synthesis costs are high. Contents of the...

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Problems solved by technology

[0003] Existing linker ligation is inefficient and expensive to synthesize

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