KIR2DS5 genotyping kit and genotyping method

Abstract

The invention discloses a KIR2DS5 genotyping kit and genotyping method. The KIR2DS5 genotyping kit comprises an amplification primer I, an amplification primer II, an amplification primer III, an amplification primer IV, an amplification primer V, an amplification primer VI, an amplification primer VII, an amplification primer VIII, a sequencing primer I, a sequencing primer II, a sequencing primer III, a sequencing primer IV, a sequencing primer V, a sequencing primer VI, a sequencing primer VII, a sequencing primer VIII, a sequencing primer IX, a sequencing primer X, a sequencing primer XI, a sequencing primer XII, a sequencing primer XIII and a sequencing primer XIV. According to the invention, PCR amplification is performed on a KIR2DS5 gene through a plurality of amplification primers, sequencing amplification is performed on an amplified PCR product through the sequencing primers, and then sequencing amplification products are sequenced, so that new allelic genes which are not liable to identify by other methods are found, and high-resolution typing of the KIR2DS5 gene is realized.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a KIR2DS5 genotyping kit and a typing method. Background technique [0002] Natural killer cells (Natural Killer cells, NK cells) in the human body through cytokines and cytotoxicity, resist infection and kill malignant cells, play an important role in innate immunity and acquired immunity. Killer cell Immunoglobulin-like Receptor (KIR) is a group of immunoglobulin-like superfamily members mainly expressed on the surface of NK cells and some T cells, which can specifically recognize human major histocompatibility Sex complex MHC-I molecules (Human Leukocyte Antigen I, HLA-I), mediate and regulate the killing function of NK cells and some effector T cells. More and more evidences from clinical transplantation and animal experiments suggest that the heterogeneous response of NK cells triggered by KIR incompatibility between donor and recipient is beneficial to the prognosis of allogen...

AI Technical Summary

Problems solved by technology

However, the above-mentioned multiple detection methods cannot achieve high-resolution typing of the KIR2DS5 gene

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