KIR2DL3 gene typing kit and typing method

Abstract

The invention discloses a KIR2DL3 gene typing kit and a KIR2DL3 gene typing method. The KIR2DL3 genotyping kit comprises an amplification primer 1, an amplification primer 2, an amplification primer 3, an amplification primer 4, an amplification primer 5, an amplification primer 6, an amplification primer 7, an amplification primer 8, a sequencing primer 1, a sequencing primer 2, a sequencing primer 3, a sequencing primer 4, a sequencing primer 5, a sequencing primer 6, a sequencing primer 7, a sequencing primer 8, a sequencing primer 9, a sequencing primer 10, a sequencing primer 11, a sequencing primer 12 and a sequencing primer 13. The KIR2DL3 gene is subjected to PCR (Polymerase Chain Reaction) amplification by virtue of multiple amplification primers, the amplified PCR product is subjected to sequencing amplification by virtue of multiple sequencing primers, and then the sequencing amplification product is sequenced, so that a new allelotype which is difficult to identify by virtue of other methods is found, and high-resolution typing of the KIR2DL3 gene is achieved.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a KIR2DL3 genotyping kit and a typing method. Background technique [0002] Natural killer cells (Natural Killer cells, NK cells) in the human body through cytokines and cytotoxicity, resist infection and kill malignant cells, play an important role in innate immunity and acquired immunity. Killer cell Immunoglobulin-like Receptor (KIR) is a group of immunoglobulin-like superfamily members mainly expressed on the surface of NK cells and some T cells, which can specifically recognize human major histocompatibility Sex complex MHC-I molecules (Human Leukocyte Antigen I, HLA-I), mediate and regulate the killing function of NK cells and some effector T cells. More and more evidences from clinical transplantation and animal experiments suggest that the heterogeneous response of NK cells triggered by KIR incompatibility between donor and recipient is beneficial to the prognosis of allogen...

AI Technical Summary

Problems solved by technology

However, due to the high degree of polymorphism of the KIR gene and the randomness of the distribution of polymorphic sites, the above-mentioned multiple detection methods have the risk of missed detection and false detection, and high-resolution typing of the KIR2DL3 gene cannot be achieved

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