KIR3DL1 genotyping kit and KIR3DL1 genotyping method

Abstract

The invention discloses a KIR3DL1 genotyping kit and a KIR3DL1 genotyping method. The KIR3DL1 genotyping kit comprises a first amplification primer, a second amplification primer, a third amplification primer, a fourth amplification primer, a first sequencing primer, a second sequencing primer, a third sequencing primer, a fourth sequencing primer, a fifth sequencing primer, a sixth sequencing primer, a seventh sequencing primer, an eighth sequencing primer, a ninth sequencing primer, a tenth sequencing primer, an eleventh sequencing primer, a twelfth sequencing primer, a thirteen sequencing primer, a fourteen sequencing primer, a fifteen sequencing primer and a sixteen sequencing primer. According to the invention, PCR amplification is carried out on a KIR3DL1 gene through a plurality of amplification primers, sequencing amplification is carried out on an amplified PCR product through a plurality of sequencing primers, and then the sequencing amplification product is sequenced, so that new allelic genes which are difficult to identify by other methods are found, and high-resolution typing of the KIR3DL1 gene is realized.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a KIR3DL1 genotyping kit and typing method. Background technique [0002] Natural killer cells (Natural Killer cells, NK cells) in the human body through cytokines and cytotoxicity, resist infection and kill malignant cells, play an important role in innate immunity and acquired immunity. Killer cell Immunoglobulin-like Receptor (KIR) is a group of immunoglobulin-like superfamily members mainly expressed on the surface of NK cells and some T cells, which can specifically recognize human major histocompatibility Sex complex MHC-I molecules (Human Leukocyte Antigen I, HLA-I), mediate and regulate the killing function of NK cells and some effector T cells. More and more evidences from clinical transplantation and animal experiments suggest that the heterogeneous response of NK cells triggered by KIR incompatibility between donor and recipient is beneficial to the prognosis of allogenei...

AI Technical Summary

Problems solved by technology

However, due to the high polymorphism of the KIR gene and the randomness of the distribution of polymorphic sites, the above-mentioned multiple detection methods have the risk of missed detection and false detection, and cannot achieve high-resolution typing of the KIR3DL1 gene

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