Method for measuring concentration of terazosin in plasma by liquid chromatography-mass spectrometry

A technology of terazosin and LC/MS, applied in the field of medicine, can solve the problems of speed, precision, sensitivity, selectivity, etc. to be improved, and achieve the effects of high sensitivity, stable baseline and good peak shape

Inactive Publication Date: 2021-05-04
徐州立顺康达医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the speed, precision, sensitivity and selectivity of the existing determination method for terazosin all need to be improved.

Method used

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  • Method for measuring concentration of terazosin in plasma by liquid chromatography-mass spectrometry
  • Method for measuring concentration of terazosin in plasma by liquid chromatography-mass spectrometry
  • Method for measuring concentration of terazosin in plasma by liquid chromatography-mass spectrometry

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0033] Example: Human K 2 Determination of Terazosin Concentration in EDTA Plasma

[0034] 1. Experimental materials and analytical equipment

[0035] Terazosin (analyte): national or equivalent, higher grade standard

[0036] Terazosin-d8 (internal standard): TLC Pharmaceutical Standards or the same or higher grade standard

[0037] The reagents used are shown in Table 1 below:

[0038] Table 1 Reagent Details

[0039]

[0040]

[0041] Note: Reagents of the same grade or higher can also be used

[0042] The analytical equipment used is shown in Table 2 below:

[0043] Table 2 Details of equipment used

[0044] components model manufacturer Binary pump (binary pump) AC Pump AB SCIEX Degasser Degasser AB SCIEX Column oven AC Column oven AB SCIEX Autosampler (auto sampler) AC Autosampler AB SCIEX Sample rack Rack Changer AB SCIEX Mass spectrometer TRIPLE QUAD TM 6500+

AB SCIEX Data processor...

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PUM

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Abstract

The invention discloses a method for measuring the concentration of terazosin in plasma by liquid chromatography-mass spectrometry. The method adopts a liquid chromatography-mass spectrometry system for determination, and comprises the following steps: firstly taking a sample to be detected, adding a certain amount of mixed organic solvent for extraction and pretreatment, separating by a chromatographic column, and detecting by a mass spectrometry detector. The method disclosed by the invention is rapid, accurate, high in sensitivity and simple and convenient to operate, and provides a basis for determining the plasma concentration of terazosin; and the linear range of a plasma standard curve of the method is 1-200 ng / mL, the intra-batch and inter-batch precision RSD is less than + / -15%, and the method is suitable for measuring the concentration of terazosin in plasma.

Description

technical field [0001] The invention belongs to the technical field of medicines, in particular to a method for determining the concentration of terazosin in blood plasma by liquid mass spectrometry. Background technique [0002] Oral administration of terazosin hydrochloride tablets is suitable for the treatment of mild or moderate hypertension. It can be used in combination with thiazide diuretics or other antihypertensive drugs, and can also be used alone when other drugs are inapplicable or ineffective. Terazosin hydrochloride mainly lowers diastolic blood pressure. Oral administration of terazosin hydrochloride tablets is also suitable for the treatment of symptoms caused by benign prostatic hyperplasia. [0003] Although the exact mechanism of the antihypertensive effect of terazosin hydrochloride has not been established so far, the relaxation of peripheral blood vessels is mainly caused by the competitive antagonism of postsynaptic α-adrenergic receptors. Terazosin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/88
CPCG01N30/06G01N30/88
Inventor 范志鸿
Owner 徐州立顺康达医药科技有限公司
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