Probiotic fermentation type eucommia ulmoides fruit and vegetable freeze-dried powder and preparation method thereof
A fermentative, probiotic technology with applications in biochemical equipment and methods, microbe-based methods, bacteria used in food preparation, etc.
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Embodiment 1
[0021] MRS liquid medium formula: peptone 10 g / L, yeast extract 5 g / L, diammonium citrate 2 g / L, glucose 20 g / L, beef extract 10 g / L, Tween 80 0.1 g / L, sulfuric acid Magnesium 0.58 g / L, manganese sulfate 0.28 g / L, dipotassium hydrogen phosphate 2 g / L, pH 6.4.
[0022] MRS solid medium formula: peptone 10 g / L, yeast extract 5 g / L, diammonium citrate 2 g / L, glucose 20 g / L, beef extract 10 g / L, Tween 80 0.1 g / L, magnesium sulfate 0.58 g / L, manganese sulfate 0.28 g / L, dipotassium hydrogen phosphate 2 g / L, 15 g / L agar, pH6.4.
[0023] A preparation method of probiotic fermented eucommia fruit and vegetable freeze-dried powder, the steps comprising:
[0024] (1) Preparation of seed liquid: pick single colonies of activated Lactobacillus reuteri, Lactobacillus plantarum, and Lactobacillus bulgaricus from the MRS solid medium, and place them in the corresponding prepared MRS liquid medium, and culture them at 30°C for 15 h , collected by centrifugation, and diluted to an OD600 value...
Embodiment 2
[0030] MRS liquid medium formula: peptone 10 g / L, yeast extract 5 g / L, diammonium citrate 2 g / L, glucose 20 g / L, beef extract 10 g / L, Tween 80 0.1 g / L, sulfuric acid Magnesium 0.58 g / L, manganese sulfate 0.28 g / L, dipotassium hydrogen phosphate 2 g / L, pH 6.4.
[0031] MRS solid medium formula: peptone 10 g / L, yeast extract 5 g / L, diammonium citrate 2 g / L, glucose 20 g / L, beef extract 10 g / L, Tween 80 0.1 g / L, magnesium sulfate 0.58 g / L, manganese sulfate 0.28 g / L, dipotassium hydrogen phosphate 2 g / L, 15 g / L agar, pH6.4.
[0032] A preparation method of probiotic fermented eucommia fruit and vegetable freeze-dried powder, the steps comprising:
[0033] (1) Preparation of seed liquid: pick single colonies of activated Lactobacillus reuteri, Lactobacillus plantarum, and Lactobacillus bulgaricus from the MRS solid medium, and place them in the corresponding prepared MRS liquid medium, and culture them at 30°C for 15 h , collected by centrifugation, and diluted to an OD600 value...
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