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Method for measuring concentration of felodipine in plasma by liquid chromatography-mass spectrometry

A technology of felodipine and LC/MS, applied in the field of medicine, can solve the problems of speed, precision, sensitivity, selectivity, etc.

Inactive Publication Date: 2021-05-11
徐州立兴佳正医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the speed, precision, sensitivity and selectivity of the existing methods for the determination of felodipine need to be improved

Method used

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  • Method for measuring concentration of felodipine in plasma by liquid chromatography-mass spectrometry
  • Method for measuring concentration of felodipine in plasma by liquid chromatography-mass spectrometry
  • Method for measuring concentration of felodipine in plasma by liquid chromatography-mass spectrometry

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0032] Example: Human K 2 Determination of Felodipine Concentration in EDTA Plasma

[0033] 1. Experimental materials and analytical equipment

[0034] Lodipine (analyte): China National Institutes for Food and Drug Control or the same, higher-grade standard Felodipine-d3 (internal standard): Toronto Research Chemicals or the same, higher-grade standard Reagents used are shown in Table 1 below :

[0035] Table 1 Reagent Details

[0036] Reagent name level manufacturer Acetonitrile (ACN) HPLC J. T. Baker Formic acid (FA) ACS Adamas Ammonium acetate (CH 3 COONH 4 )

HPLC J. T. Baker

[0037] Note: Reagents of the same grade or higher can also be used

[0038] The analytical equipment used is shown in Table 2 below:

[0039] Table 2 Details of equipment used

[0040] components model manufacturer Binary pump (binary pump) AC Pump AB SCIEX Degasser Degasser AB SCIEX Column oven AC Column...

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Abstract

The invention discloses a method for measuring the concentration of felodipine in plasma by liquid chromatography-mass spectrometry. The method adopts a liquid chromatography-mass spectrometry system for measurement, and comprises the following steps: firstly taking a sample to be detected, adding a certain amount of mixed organic solvent for extraction and pretreatment, separating by a chromatographic column, and detecting by a mass spectrometry detector. The method disclosed by the invention is rapid, accurate, high in sensitivity and simple and convenient to operate, and provides a basis for measuring the blood concentration of felodipine; and the linear range of the plasma standard curve of the method is 0.02-4 ng / mL, the intra-batch and inter-batch precision RSD is less than + / -15%, and the method is suitable for measuring the concentration of felodipine in the plasma.

Description

technical field [0001] The invention belongs to the technical field of medicines, in particular to a method for measuring medicines, in particular to a method for measuring the concentration of felodipine in blood plasma by liquid mass spectrometry. Background technique [0002] Felodipine is an effective drug for the treatment of high blood pressure, and this tablet can be used alone or in combination with other antihypertensive drugs. Hypertension is one of the important risk factors for atherosclerosis and vascular remodeling, and there is a close relationship between hypertension and endothelial diastolic dysfunction. The treatment goal of modern hypertension emphasizes the reduction of blood pressure, while at the same time, attention should be paid to reversing the remodeling of blood vessels, improving the function protection of vascular endothelial cells or restoring the damaged endothelial function, so as to greatly reduce the occurrence of clinical cardiovascular a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 范志鸿
Owner 徐州立兴佳正医药科技有限公司