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Differentiation and cultivation process of tissue cultured potato seedling

A cultivation method and potato technology, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of easy pollution of cultures, high cost of tissue culture seedlings, low emergence rate, etc.

Inactive Publication Date: 2003-11-19
SHIJIAZHUANG INST OF AGRI MODERNIZATION CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The culture medium accounts for about 40% of the culture cost in conventional differentiated seedling cultivation methods, and the culture is easily polluted, the emergence rate per unit area is low, and the cost of tissue culture seedlings is high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0009] The present invention will be further described below in conjunction with the example that virus-free potato tissue culture shoots produce:

[0010] 1. Take a 250ml small canned bottle as a culture container, wash it before use, lay a piece of filter paper inside, seal it and sterilize it under high temperature and high pressure for later use;

[0011] 2. Preparation of the stem section treatment solution: prepare a solution containing 1.5 times of conventional culture medium, micronutrients, 5% sucrose, 5ppmIAA, pH5.8, put it into a jar, seal it, sterilize it under high temperature and high pressure, and let it cool reserve;

[0012] 3. Preparation of stem segments: select differentiated seedlings that have been differentiated and cultivated for more than two generations, and grow for about 20-30 days, take them out of the test tube on a super-clean bench, and cut them into 1-1.5cm long stems with 1-2 buds section, quickly put it into the sterilized treatment solution...

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PUM

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Abstract

The present invention provides substrate less and culture medium alternatively, multitimes, multigeneration cultivation technology for reducing the production cost of detoxified potato group cultivating seedling, said method includes putting differentiated seedling alternatively under substrate less cultivating condition and culture medium condition to proceed multitimes and multigenerations differentiation cultivation. Said substrate less cultivation condition includes temperature 25 plus or minus 2 deg.C illumination 2000-3000 lux, illumination time 14-16 hrs, cultivate 18-20 day to implement one generation differentiation, then transfer to culture meidum cultivaltino. Advantages include saving culture meidum near one half, greatly reduce the production cost of group seedling cultivation.

Description

technical field [0001] The invention relates to a method for differentiating and cultivating potato tissue-cultured seedlings, which belongs to the technical field of plant cultivation methods, and is especially suitable for industrialized rapid propagation of potato tissue-cultured and differentiated seedlings. Background technique [0002] Virus-free potatoes can effectively increase yield and improve quality, and virus-free seed potatoes are becoming more and more popular among potato growers. However, since the virus-free original seed potato must be obtained through tissue culture, and the tissue culture technology requires a relatively high investment, the production cost of the original virus-free potato is too high. This causes the cultivator to be unable to bear the excessively high seed potato price or the producer of the original original seed potato cannot maintain production due to the low profit, which makes it difficult to apply the original original seed pota...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 冯学赞安忠民王静焦顺兴
Owner SHIJIAZHUANG INST OF AGRI MODERNIZATION CHINESE ACAD OF SCI