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Compound C extracted from wine-processed cornel and having effect of reducing cholesterol, and preparation method and application thereof

A technology of wine cornel and cholesterol, applied in the field of medicine

Active Publication Date: 2021-05-28
HENAN UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the active ingredient of the compound extracted from Cornus officinalis for lowering cholesterol and treating coronary heart disease has not been publicly reported so far.

Method used

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  • Compound C extracted from wine-processed cornel and having effect of reducing cholesterol, and preparation method and application thereof
  • Compound C extracted from wine-processed cornel and having effect of reducing cholesterol, and preparation method and application thereof
  • Compound C extracted from wine-processed cornel and having effect of reducing cholesterol, and preparation method and application thereof

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specific Embodiment approach

[0024] The specific implementation of the present invention will be described in detail below in combination with specific situations.

[0025] The present invention can be provided by the following examples in concrete implementation.

Embodiment 1

[0027] A kind of compound C with cholesterol-lowering effect extracted from Cornus officinalis of the present invention is Cornusgallates C (compound 1), and molecular structural formula is:

[0028]

[0029] Its preparation method is, comprises the following steps:

[0030] (1) Take 10kg of dried Cornus officinalis, add 100L of water, soak at 4°C for 10 days, filter, combine the extracts, concentrate under reduced pressure to a sample solution equivalent to 0.6g / mL crude drug;

[0031] (2) Load the above sample solution on a HP-20 chromatographic column with a diameter-to-height ratio of 1:10, and then elute with 20 L of water and 40 L of ethanol with a mass concentration of 30% in sequence, at a flow rate of 5 mL / min, and collect the 30% ethanol parts for washing Deliquified, concentrated under reduced pressure to obtain an extract Fr.30% EtOH (520g) with a relative density of 1.2 to 1.4 at 50°C;

[0032] (3) The extract Fr.30% EtOH was chromatographed by TOYOPEARL HW-40...

Embodiment 2

[0036] A kind of preparation method of the compound C with lowering cholesterol effect that the present invention extracts from Fructus Cornus officinalis comprises the following steps:

[0037] (1) Take 10kg of dried Cornus officinalis, add 60L of water, soak at 4°C for 14 days, filter, combine the extracts, and concentrate under reduced pressure to a concentrated solution equivalent to crude drug 1.8g / mL;

[0038] (2) Load the concentrated solution on the HP-20 chromatographic column with a diameter-to-height ratio of 1:8, and then elute with 12L of water and 24L of ethanol with a mass concentration of 30% in sequence. solution, concentrated under reduced pressure to obtain an extract Fr.30% EtOH (515g) with a relative density of 1.2 to 1.4 at 50°C;

[0039] (3) The extract Fr.30%EtOH is chromatographed on TOYOPEARL HW-40C column, r=2cm, H=60cm, eluting with methanol, flow rate 2mL / min, elution of 4 column volumes, collecting the same components, reducing Concentrate under ...

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Abstract

The invention relates to a compound C extracted from wine-processed cornel and having an effect of reducing cholesterol, and a preparation method and application thereof. The problems of separation and preparation of a new compound from wine-like cornel and application of the new compound in preparation of medicines for reducing cholesterol and treating coronary heart disease are effectively solved.,Water is added to wine-processed cornel, reduced pressure concentration is reduced, the obtained material is added into an HP-20 chromatographic column, elution is performed with water and 30% ethanol, 30% ethanol eluate is collected, concentrating is performed under reduced pressure to obtain extract Fr.30% EtOH, the extract Fr.30% EtOH undergoesTOYOPEARL HW-40C column chromatography and methanol elution, the same components are collected, and are concentrated under reduced pressure to obtain extract Fr.ETH-2, Flash-ODS column chromatography is performed, methanol-water gradient elution is performed, 30: 70 eluate is collected, concentrating is performed under reduced pressure to obtain extract Fr.ETH-2-3, further separation is performed on semi-preparative HPLC, the volume ratio of acetonitrile to water is 20: 80, the flow velocity is 3mL / min, and a substance with a chromatographic peak in 25min is collected to obtain the compound C. The method is easy to operate, high in guidance quality and high in product purity, and the new application and the medicinal value of the wine-processed cornel are developed.

Description

technical field [0001] The invention relates to the field of medicine, in particular to a compound C with cholesterol-lowering effect extracted from Cornus officinalis and its preparation method and application. Background technique [0002] Statistics show that the number of people suffering from coronary heart disease in China is about 290 million. Cholesterol accumulation is a key risk factor for coronary heart disease (CHD). CHD is the number one killer of human health; CHD accounts for one in every six deaths in the United States. There are many drugs on the market to maintain cholesterol homeostasis, such as the classic cholesterol synthesis inhibitors statins, cholesterol absorption inhibitors ezetimibe, and Duoxikang, which promotes cholesterol excretion, and so on. However, the morbidity and mortality of CHD are still increasing year by year, and there are still serious side effects. In the etiology and pathogenesis of coronary heart disease, Qi deficiency, Yang ...

Claims

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Application Information

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IPC IPC(8): C07D311/76A61P3/06A61P9/10
CPCC07D311/76A61P3/06A61P9/10
Inventor 许二平李红彬张婷婷张玲霞代丽萍陈随清王智民
Owner HENAN UNIV OF CHINESE MEDICINE
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