Liquid chromatography-mass spectrometry for determining atrazine, imidacloprid and metabolites thereof in leaves

Abstract

The invention belongs to the technical field of plant metabolite detection, and discloses a liquid chromatography-mass spectrometry method for determining atrazine, imidacloprid and metabolites thereof in leaves. The method comprises the steps of: using a QuEChERS method for analyzing various influence factors in the process of determining atrazine, imidacloprid and metabolite residues in plant leaves, and combining an HPLC-MS-MS method for detection; adding acetonitrile, carrying out vortex mixing, adding a salting-out material, carrying out vortex uniform mixing, and carrying out ultrasonic extraction and centrifugation at room temperature; and adding the supernatant into a centrifugal tube, adding a purifying agent anhydrous sodium sulfate and C18 for purification, uniformly mixing in a vortex manner, passing the supernatant through an organic phase microfiltration membrane, absorbing the filtrate, and determining by using a liquid chromatography mass spectrometer. The method has the advantages of simplicity and convenience in operation, good purification effect, high recovery rate, high sensitivity and the like, and can provide theoretical basis and technical support for monitoring residues of pesticides atrazine, imidacloprid and metabolites thereof in northeast black land agricultural crops and plant samples.

Description

technical field [0001] The invention belongs to the technical field of plant metabolite detection, and in particular relates to a liquid chromatography mass spectrometry method for determining atrazine, imidacloprid and their metabolites in leaves. Background technique [0002] Atrazine (atrazine) chemical name: 2-chloro-4-diethylamino-6-isopropylamino-1,3,5-triazine is a triazine herbicide mainly used in corn It can control various broad-leaved weeds and annual grass weeds in crop fields such as , sorghum and sugarcane, and also has a certain inhibitory effect on perennial weeds. Due to its long residual period, high water solubility, large application amount and wide application range, atrazine has increasingly prominent impact on the environment. The U.S. Department of Health and Social Services reported that long-term exposure to atrazine can affect the human immune system, lymphatic system, reproductive system, and endocrine system, which may cause deformities and indu...

AI Technical Summary

Problems solved by technology

[0009] (1) The determination of atrazine is mostly concentrated in the determination of residues in soil and water quality, and the determination of atrazine residues in plants is less, and the sample processing process is long and complicated, the amount of organic solvent is large, and the purification effect is poor

[0010] (2) The mensuration of imidacloprid mostly concentrates on the residue of imidacloprid itself in food, and the research of its metabolite is less, and the research of four kinds of metabolites in the plant that the present invention mentions does not see simultaneously

[0011] (3) The determination methods in the prior art are complicated and have poor accuracy, which cannot satisfy the simultaneous determination of atrazine, imidacloprid and their metabolites in plant samples

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