In-vivo and in-vitro multi-component analysis method of fangji huangqi decoction
A technology of Fangji Huangqi Decoction and active ingredients is applied in the field of in vitro and in vivo multi-component analysis of Fangji Huangqi Decoction, which can solve the problems of complex formula components, difficult to reflect the overall level of the compound, and insufficient research on Fangji Huangqi Decoction, etc. The effect of good durability, high sensitivity and high analysis efficiency
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Embodiment 1
[0027] Quantitative analysis of 18 active ingredients in Fangji Huangqi Decoction in Example 1
[0028] 1 Instruments, materials and reagents
[0029] 1.1 Instrument
[0030] Dionex UPLC 3000 ultra-high performance liquid phase-TSQ quantum Access MAX triple quadrupole mass spectrometry system (Thermo, USA);
[0031] Waters Acquity UPLC BEH C18 column (2.1*100mm 1.7μm, Waters, USA);
[0032] FA2004N electronic balance (Shanghai Precision Scientific Instrument Co., Ltd.);
[0033] CP225D electronic balance (Sartorius AG, Shanghai Precision Scientific Instrument Co., Ltd.);
[0034] Milli-Q type ultrapure water meter (Millipore company, the United States);
[0035] YC-18X Joyoung Induction Cooker (Shandong Joyoung Small Appliance Co., Ltd.);
[0036] SK250LHC ultrasonic cleaner (Shanghai Kedao Ultrasonic Instrument Co., Ltd.);
[0037] Micropipette 0.1-10 μL, 10-100 μL, 20-200 μL, 200-1000 μL (Eppendorf, Germany);
[0038] Centrifuge 5427R refrigerated centrifuge (Eppendor...
Embodiment 2
[0120] The establishment of the UPLC-MS / MS analytical method of multi-component simultaneous determination in embodiment 2 rat plasma
[0121] 1 Instruments, reagents, materials and animals
[0122] 1.1 Instrument
[0123] Dionex UPLC 3000 ultra-high performance liquid phase-TSQ quantum Access MAX triple quadrupole mass spectrometry system (Thermo, USA);
[0124] Waters Acquity UPLC BEH C18 column (2.1*100mm, 1.7μm, Waters, USA);
[0125] FA2004N electronic balance (Shanghai Precision Scientific Instrument Co., Ltd.);
[0126] Milli-Q ultrapure water device (Millipore company, the United States);
[0127] LV concentrator (Biotage, USA);
[0128] QT-1 vortex mixer (Shanghai Qite Analytical Instrument Co., Ltd.);
[0129] Centrifuge 5427R refrigerated centrifuge (Eppendorf, Germany);
[0130] CP225D electronic balance (Sartorius AG, Shanghai Precision Scientific Instrument Co., Ltd.);
[0131] SK250LHC ultrasonic cleaner (Shanghai Kedao Ultrasonic Instrument Co., Ltd.);...
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