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Method for detecting content of total saponins in conyza blinii and quality control method

A technology of golden gentian grass and detection method, which is applied in the direction of color/spectral property measurement, etc., can solve the problems such as the inability to accurately grasp the quality of golden gentian grass and the lack of total saponins of golden gentian grass.

Inactive Publication Date: 2021-06-01
CHENGDU HUASUN GRP INC LTD
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  • Application Information

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Problems solved by technology

[0005] In order to solve the technical problem that there is no detection method for the total saponins of Gentiana in the above background technology and the quality of Gentiana cannot be accurately grasped, the present invention provides a detection method and a quality control method for the content of total saponins in Gentiana

Method used

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  • Method for detecting content of total saponins in conyza blinii and quality control method

Examples

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Embodiment 1

[0035] A detection method for total saponin content in Gentiana chinensis, comprising the following steps:

[0036] S1. Take 15 mg of ginsenoside Re reference substance, put it in a 100ml measuring bottle, add methanol to dissolve and dilute to the mark, shake well, and obtain the reference substance solution (containing 0.15 mg of ginsenoside Re in every 1 ml);

[0037] S2. Take by weighing 200 μl, 400 μl, 600 μl, 800 μl, and 1000 μl of the reference substance solution in step S1, respectively, put them in 10 ml stoppered test tubes, evaporate the solvent, and precisely add 5% vanillin glacial acetic acid solution-perchloric acid ( 2:8) (newly prepared) mixed solution 1ml, placed in a 60°C water bath for 15 minutes, taken out and cooled in an ice water bath, precisely added 5ml of glacial acetic acid, shaken, immediately according to the colorimetric method, at a wavelength of 555nm The absorbance is measured at the place where the absorbance is taken as the ordinate, and the...

Embodiment 2

[0048] The difference with Example 1 is that, in step S3, the Gentiana gentiana sample 2 to be tested is taken to make 20 g of powder and then 20 ml of water is added, and the content of the total saponins obtained is as follows in Table 3:

[0049] table 3

[0050] sample Concentrationmg / ml Absorbance content% sample 2 0.003237 0.0443 0.467％

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Abstract

The invention relates to the field of conyza blinii detection, in particular to a method for detecting the content of total saponins in conyza blinii and a quality control method. A determination method for detecting the total saponins in conyza blinii by adopting ultraviolet-visible spectrophotometry is established, and the total saponins are calculated by taking ginsenoside Re as a reference substance, so that the product quality of conyza blinii extract and extract tablets can be better controlled. The technical problem that the quality of conyza blinii cannot be accurately mastered due to the fact that a conyza blinii total saponin detection method does not exist in a conyza blinii medicinal material at present is solved.

Description

technical field [0001] The invention belongs to the detection field of gentian, and in particular relates to a detection method and a quality control method for total saponin content in gentian. Background technique [0002] Golden gentian (scientific name: bear bile grass, Latin scientific name: Conyza blinii) is an annual herbaceous plant of the genus Baijiu in the family Asteraceae. Produced in Yunnan, Sichuan and Guizhou. It often grows on hillside grasslands, wasteland roadsides or wilderness at an altitude of 1800-2600 meters. The whole herb is used for medicinal purposes, and has the effects of anti-inflammatory, hemostasis, and anti-malaria. [0003] Gentiana is the dry aerial part of Artemisia annua of Compositae. In the quality standard recorded in "Chinese Pharmacopoeia", there is only the detection method of artemisinin, so the content of artemisinin can be measured by this standard. [0004] However, the total saponins are the main medicinal components of Ge...

Claims

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Application Information

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IPC IPC(8): G01N21/31G01N21/33
CPCG01N21/31G01N21/33
Inventor 郑洪军郭兆鹏谢学江
Owner CHENGDU HUASUN GRP INC LTD
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