Preparation method of fish skin collagen peptide

A technology of fish skin collagen and fish skin, which is applied in the field of preparation of fish skin collagen peptides, can solve the problems of many impurities and the purity needs to be further improved

Inactive Publication Date: 2021-06-04
德兰梅勒(北京)分离技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the fish skin collagen peptide obtained by the prior art contains many impurities such as salt, raw material slag, and suspended matter, and its purity needs to be further improved.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0026] The invention provides a kind of preparation method of fish skin collagen peptide, comprises the following steps:

[0027] 1) Soaking the fish skin in an alkali solution and an acid solution in turn to obtain a pretreated fish skin; carrying out water extraction on the pretreated fish skin to obtain a gelatin extract;

[0028] 2) mixing the gelatin extract and protease for enzymolysis, and inactivating the enzyme to obtain an enzymolysis solution;

[0029] 3) Filtrating the enzymolysis solution to obtain a filtrate; the sieve aperture of the filtration is 1 μm;

[0030] 4) performing ultrafiltration on the filtrate, and collecting the ultrafiltrate; the molecular weight cut-off of the ultrafiltration is 7000-8000Da;

[0031] 5) decolorizing the ultrafiltrate to obtain a decolorizing solution;

[0032] 6) Nanofiltration is performed on the decolorization solution, and the nanofiltration solution is collected; the nanofiltration solution contains fish skin collagen pept...

Embodiment 1

[0052] 1) Cut 400kg of tilapia skin into pieces, put it into the acid-base pool, add 6t of 0.05mol / L NaOH aqueous solution into the acid-base pool, and soak for 3 hours continuously. Add 6t of 0.2% H by volume 2 SO 4 Aqueous solution, soak for 3 hours, and replace the acid solution every hour. Add 8t of clear water, adjust the temperature to 50°C, and soak for 6h.

[0053] 2) After pretreatment, add the gelatin extract into the enzymolysis tank, add 2kg of alkaline protease, adjust the pH to 8.0, the temperature is 50°C, enzymolysis for 2 hours, stir until the end of the enzymolysis, raise the temperature to 100°C, and inactivate at high temperature for 10 minutes .

[0054] 3) Filter the enzymatic hydrolysis solution using a plate and frame filter press to remove substances with a size larger than 1 μm to obtain a filtrate.

[0055] 4) The filtrate enters the inorganic ceramic membrane ultrafiltration system, the ultrafiltration molecular weight cut-off is 7000-8000Da, an...

Embodiment 2

[0060] 1) Cut 400kg of tilapia skin into pieces, put it into the acid-base pool, add 6t of 0.06mol / L NaOH aqueous solution into the acid-base pool, and soak for 3.5 hours continuously. Add 6t of 0.3% H by volume 2 SO 4 Aqueous solution, soak for 3.5 hours, and replace the acid solution every hour. Add 8t of clear water, adjust the temperature to 50°C, and soak for 6h.

[0061] 2) After pretreatment, add the gelatin extract into the enzymolysis tank, add 2kg of alkaline protease, adjust the pH to 8.0, the temperature is 50°C, enzymolysis for 2 hours, stir until the end of the enzymolysis, raise the temperature to 100°C, and inactivate at high temperature for 10 minutes .

[0062] 3) Filter the enzymatic hydrolysis solution using a plate and frame filter press to remove substances with a size larger than 1 μm to obtain a filtrate.

[0063] 4) The filtrate enters the inorganic ceramic membrane ultrafiltration system, the ultrafiltration molecular weight cut-off is 7000-8000Da...

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PUM

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Abstract

The invention provides a preparation method of fish skin collagen peptide, and belongs to the technical field of marine collagen peptide preparation. The preparation method comprises the following steps of: sequentially soaking fish skin in an alkaline solution and an acid solution for pretreatment, then performing water extraction, and transferring effective components in the fish skin into a leaching solution to obtain a gelatin leaching solution; performing enzymolysis on the gelatin leaching solution, and effectively performing enzymolysis on macromolecular protein into peptide substances to obtain an enzymatic hydrolysate; filtering to remove raw material residues and suspended matters in the enzymatic hydrolysate; performing ultrafiltration on an enzymatic hydrolysate filtrate to separate collagen peptide with the molecular weight of 7000-8000Da; and decolorizing an ultrafiltrate, then performing nanofiltration desalination, and intercepting collagen peptide with the molecular weight of 200-1000Da to obtain the fish skin collagen peptide. According to the preparation method provided by the invention, impurities with different molecular sizes are removed step by step in multiple links, so that the impurities in the collagen peptide can be effectively reduced.

Description

technical field [0001] The invention relates to the technical field of preparation of marine collagen peptides, in particular to a preparation method of fish skin collagen peptides. Background technique [0002] Collagen is a protein composed of three peptide chains intertwined to form a helical structure, which widely exists in animals. Collagen peptide is a product between amino acids and proteins formed after the peptide bond of aquatic collagen is broken under the action of enzymes, microorganisms, and chemicals. [0003] Fish body scraps contain a lot of collagen, and the collagen content in fish skin accounts for more than 80% of the total protein, which is much higher than other parts of fish body. Therefore, using fish skin as a raw material to extract collagen peptides can make full use of resources and increase the added value of fish processing. [0004] At present, the common extraction method of fish skin collagen peptide is alkaline protease hydrolysis extrac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K14/78C07K1/34
CPCC12P21/06C07K14/78
Inventor 苏战华苏仲民王晓强
Owner 德兰梅勒(北京)分离技术有限公司
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