Precise prevention and control method for infection spleen and kidney necrosis virus disease of micropterus salmoides

A spleen and kidney necrosis virus, infectious technology, applied in the field of precise prevention and control of California sea bass infectious spleen and kidney necrosis virus disease, can solve problems such as lack of preventive measures

Inactive Publication Date: 2021-06-08
广州力必拓生物科技有限公司
View PDF8 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, most of the disease prevention and control technologies for aquatic animals, including California perch, are only satisfied with the detection and detection of related pathogens, and there is a serious lack of a series of comprehensive technologies for corresponding prevention and control measures. It is urgent to establish "quantitative rapid detection + traditional Chinese medicine technology intervention (or microecological intervention) "A precise approach to disease prevention and control

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Precise prevention and control method for infection spleen and kidney necrosis virus disease of micropterus salmoides
  • Precise prevention and control method for infection spleen and kidney necrosis virus disease of micropterus salmoides

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] On December 22, 2020, a farmer in Foshan died of California perch that had been breeding for about 20 days, and the number of deaths per day was about 600. Later, it was detected by fluorescent quantitative PCR that the death was caused by infectious spleen and kidney necrosis virus, and the treatment was as follows Program processing:

[0044] First stop feeding the feed.

[0045] On the 1st and 2nd day, use "Shanze No. 1 500mL / mu Shibencao Shenlu 1000mL / mu" for two consecutive days;

[0046] On the 3rd and 4th day, use "Shanduqing No. 2 40g / mu" for two consecutive days.

[0047] On the 5th and 6th days, use "povidone-iodine solution (aquaculture) 330mL / mu+herbal dew 1000mL / mu for two consecutive days.

[0048] Feedback from customers: The number of deaths within 6 days after taking the drug changed significantly: from the initial more than 600 deaths per day to a few deaths per day, the death rate finally dropped to zero.

Embodiment 2

[0050] On September 8, 2020, a farmer in Kaifeng, Henan Province, died of California perch bred for about 2 months. The number of deaths per day was about 800. Later, it was detected by fluorescent quantitative PCR that the death was caused by infectious spleen and kidney necrosis virus. According to the The following treatment options address:

[0051] First stop feeding the feed.

[0052] On the 1st and 2nd day, use "Shanze No. 1 500mL / mu Shibencao Shenlu 1000mL / mu" for two consecutive days;

[0053] On the 3rd and 4th day, use "Shanduqing No. 2 40g / mu" for two consecutive days.

[0054] On the 5th and 6th days, use "povidone-iodine solution (aquaculture) 330mL / mu+herbal dew 1000mL / mu for two consecutive days.

[0055] Customer feedback: Changes in the number of deaths within 6 days after medication: 800 / 400 / 200 / 100 / dozens / several, and the death rate was finally reduced to less than a few.

Embodiment 3

[0057] On August 25, 2020, a farmer in Zhongshan, Guangdong, died of California perch bred for about 3 months. The number of deaths per day was about 400. Later, it was detected by fluorescent quantitative PCR that the death was caused by infectious spleen and kidney necrosis virus. According to the The following treatment options address:

[0058] First stop feeding the feed.

[0059] On the 1st and 2nd day, use "Shanze No. 1 500mL / mu Shibencao Shenlu 1000mL / mu" for two consecutive days;

[0060] On the 3rd and 4th day, use "Shanduqing No. 2 40g / mu" for two consecutive days.

[0061] On the 5th and 6th days, use "povidone-iodine solution (aquaculture) 330mL / mu+herbal dew 1000mL / mu for two consecutive days.

[0062] Customer Feedback: The number of deaths within 6 days after taking the drug changed significantly: from the initial death rate of more than 400 per day, the number of deaths was finally reduced to a few per day.

[0063] From the above case analysis, it can be c...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a precise prevention and control method for an infection spleen and kidney necrosis virus disease of micropterus salmoides. According to the scheme, a specific DNA nucleic acid fragment of an infection spleen and kidney necrosis virus of the micropterus salmoides is quantitatively detected by employing a magnetic-bead-method viral DNA / RNA nucleic acid extraction kit (LBT-VDEK), utilizing a 16-pore fluorescent quantitative PCR instrument (LBT-16QPCR) and associated facilities thereof and employing a PCR-fluorescent probe technique, and the scheme is simple, convenient and rapid and is good in specificity and high in sensitivity; and in view of the detected micropterus salmoides carrying over the infection spleen and kidney necrosis virus, modern traditional Chinese medicine technologies and microbiologic technologies are applied to prevention and treatment, the death rate can be lowered effectively, precise prevention and control are achieved, the infection spleen and kidney necrosis virus disease of the micropterus salmoides is solved comprehensively, systematically and globally, and thus, the method has a very broad application prospect and a very high practical value.

Description

technical field [0001] The invention relates to aquatic animal disease prevention and control technology, and mainly aims at the precise prevention and control method of California perch infectious spleen and kidney necrosis virus disease. Background technique [0002] California perch is a famous freshwater cultured species in my country. With the success of the artificial propagation and seedling technology of California perch, the breeding industry of California perch has developed rapidly, and at the same time, the problem of diseases has become increasingly serious. The diseases of California perch mainly include viral diseases, bacterial diseases and parasitic diseases. Among them, the viral disease spreads rapidly and has a high incidence rate, and there are no effective control measures at present, which seriously affects the development of California perch aquaculture. In recent years, large-scale explosive epidemics have occurred in farmed California perch, resul...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/6806C12Q1/686C12N15/10
CPCC12Q1/701C12Q1/6806C12Q1/686C12N15/1013C12Q2563/107C12Q2545/114
Inventor 黄文文黄志坚袁永青陈总会
Owner 广州力必拓生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap