Breeding method of bacterial wilt resistant male sterile eggplant rootstock

Abstract

The invention discloses a breeding method of a bacterial wilt resistant male sterile eggplant rootstock. The breeding method comprises the following steps that a cytoplasmic male sterile and parthenocarpy strain serving as a female parent and a high bacterial wilt resistant strain 1 serving as a male parent are hybridized to obtain a transformation F1, three-generation backcross is carried out to obtain a transformation BC3, a high bacterial wilt resistant plant is selected from the transformation BC3, the high bacterial wilt resistant plant is backcrossed with the high bacterial wilt resistant strain 1 to obtain a CMSBW2; and the CMSBW2 serving as a female parent and a high bacterial wilt resistant strain 2 serving as a male parent are taken to hybridize to obtain a hybridized combination CMSRS1, namely the bacterial wilt resistant male sterile eggplant rootstock. According to the breeding method, the breeding process is shortened, the selection difficulty of intermediate generations is reduced, the identification frequency of bacterial wilt resistance is reduced, the hybrid seed production procedure is simplified, and the obtained bacterial wilt resistant male sterile eggplant rootstock has the sterility degree of 100%, the obtained bacterial wilt resistant male sterile eggplant rootstock is vigorous in growth vigor and high in bacterial wilt resistance, capable of being used as the rootstocks for grafting eggplants, and the breeding method has important significance and good application prospects in eggplant production.

Description

technical field [0001] The invention relates to the field of plant breeding, in particular to a breeding method for bacterial wilt-resistant male sterile eggplant rootstocks. Background technique [0002] Eggplant (Solanum melongena) is a self-pollinated plant with a natural outcrossing rate of about 10%. Because of its obvious heterosis, hybrid seeds account for about 80% of eggplant production. In the production of hybrid seeds, artificial emasculation and pollination at the bud stage are required, which is time-consuming and labor-intensive, and the production cost is high. If emasculation is not timely or complete, it is easy to form "false hybrids" to reduce seed purity and increase the risk of parental loss. At present, there are many eggplant male sterile materials that have been found, but the application of restorer lines has not been reported, and eggplant is a crop whose fruit is the edible part. Without a suitable restorer line, it means that the produced species...

AI Technical Summary

Problems solved by technology

In the production of hybrid seeds, artificial emasculation and pollination at the bud stage are required, which is time-consuming and labor-intensive, and the production cost is high. If emasculation is not timely or complete, it is easy to form "false hybrids" to reduce seed purity and increase the risk of parental loss.

At present, there are many eggplant male sterile materials that have been found, but the application of restorer lines has not been reported, and eggplant is a crop whose fruit is the edible part. Without a suitable restorer line, it means that the produced species cannot set fruit normally and are not edible. value

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