Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Humanized glioma pdx mouse model and its preparation method and application

A mouse model, glioma technology, applied in the field of tumor, can solve the problems of inability to fully evaluate anti-tumor efficacy, lack of immune system, etc.

Active Publication Date: 2022-05-24
XIANGYA HOSPITAL CENT SOUTH UNIV
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The present invention provides a humanized glioma PDX mouse model to solve the problem that the usual tumor model uses immunodeficiency animals to transplant human tumor cells or tissues. Due to the lack of a complete immune system, the anti-tumor efficacy cannot be completely evaluated. technical problem

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Humanized glioma pdx mouse model and its preparation method and application
  • Humanized glioma pdx mouse model and its preparation method and application
  • Humanized glioma pdx mouse model and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0030] The preparation method of the humanized glioma PDX mouse model of this embodiment includes the following steps:

[0031] S1. Construction of nude mouse glioma model: The patient-derived glioma cells were implanted into mice;

[0032] S2. Culture: Raise mice until the glioma cell volume grows to 62mm 3 ~100mm 3 ;

[0033] S3. Construction of humanized glioma PDX mouse model: The mice were irradiated with sub-lethal doses of X-rays and transplanted with syngeneic functional lymphocytes to detect the growth of glioma cells and lymphocytes in mice The proliferation and differentiation of humanized glioma PDX mouse model was obtained.

[0034] The preparation method of the humanized glioma PDX mouse model, which expresses the patient's immune system while loading human glioma. This model provides tumor cells with a growth microenvironment more similar to the human body. Expression, gene mutation, inflammation and treatment response truly and accurately reflect the perfor...

Embodiment 1

[0046] The preparation method of humanized glioma PDX mouse model includes the following steps:

[0047] S1. Construction of nude mouse glioma model: preparation of NRG (NOD.CgRag1 tmiMom IL2rg tm1wjl / Sz) mice, the tumor sphere cultures of fresh surgical specimens from patients were centrifuged at a centrifugal force of 200 × g for 5 min, the precipitate was digested into single cells with Accutase cell digestion solution, and re-prepared in ice-cold PBS The cell suspension is ready for use, and the concentration of the cell suspension is 5 × 10 7 / ml, drill the skull at 1mm to 2mm lateral to the intersection of the midline and the coronal suture and 1mm anterior to the coronal suture, draw the cell suspension into the Hamilton syringe and needle on the stereotaxic instrument, and align the needle with the center of the skull hole. Before starting the injection Wait for 1 min, and inject the cell suspension into the mouse brain at a rate of 1 μL / min. The injection volume o...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a humanized glioma PDX mouse model. The humanized glioma PDX mouse model is obtained by transplanting human glioma cells and functional lymphocytes homologous to the glioma cells. The humanized glioma PDX mouse model of the present invention is obtained by transplanting human glioma cells and glioma cell homologous functional lymphocytes. Immunodeficient mice are transplanted with lymphocytes to make them have human immune function, so that the mice can express the patient's immune system while bearing human glioma, which can simulate the interaction between human tumor cells and the immune system, It has important application prospects in the development and preclinical evaluation of tumor immunotherapy drugs. Moreover, the humanized glioma PDX mouse model has a high ability to induce differentiation of T cells, which provides an important research tool for the development of glioma immunotherapy, new drugs and new equipment.

Description

technical field [0001] The present invention relates to the field of tumors, in particular, to a humanized glioma PDX mouse model. In addition, the present invention also relates to a preparation method and application comprising the above-mentioned humanized glioma PDX mouse model. Background technique [0002] For a long time, in order to study tumors, it is customary to transplant tumor cell lines into immunodeficient mice to establish tumor models. However, due to the residual immune system of mice and the lack of human's own immune system, the experimental results deviate greatly from the clinical reality. Moreover, with the development of science and technology, tumor immunotherapy is another new treatment method that can improve the survival of tumor patients after surgery, radiotherapy, chemotherapy and molecular targeted therapy. Further research on tumor immunotherapy requires the establishment of animal models of the interaction between human tumors and the huma...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/027A61K49/00
CPCA01K67/0271A61K49/0008A01K2207/12A01K2227/105A01K2267/0325A01K2267/0387A01K2267/0393
Inventor 刘志雄罗承科许胜超肖志强陈凌陈新建
Owner XIANGYA HOSPITAL CENT SOUTH UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com