Fingerprint spectrum construction method and detection method of Guizhishaoyaozhimu decoction composition

A fingerprint and construction method technology, which is applied to the fingerprint construction method and detection field of Guizhi Shaoyao Zhimu Decoction composition, can solve the problems of inability to control quality and affect the effectiveness of quality control, and achieve improved accuracy and good response effect of effect

Active Publication Date: 2021-06-29
SINOPHARM GUANGDONG GLOBAL PHARMA CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, Guizhi Shaoyao Zhimu Decoction is made of nine herbs, and there are dozens or even hundreds of chemical components in it. Only eight chemical reference substances are used to test the quality of the medicine, which cannot ...

Method used

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  • Fingerprint spectrum construction method and detection method of Guizhishaoyaozhimu decoction composition
  • Fingerprint spectrum construction method and detection method of Guizhishaoyaozhimu decoction composition
  • Fingerprint spectrum construction method and detection method of Guizhishaoyaozhimu decoction composition

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Experimental program
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Effect test

Embodiment 1

[0170] Paeoniae Alba reference substance solution: Take an appropriate amount of gallic acid, catechin, paeonifloride glycoside, paeoniflorin, 1,2,3,4,6-O-pentagalloyl glucose reference substance, accurately weighed, add methanol to prepare Make a solution containing 100 μg of gallic acid, 100 μg of catechin, 10 μg of paeoniflorin, 20 μg of paeoniflorin, and 80 μg of 1,2,3,4,6-O-pentagalloyl glucose per 1 ml, as a mixed reference solution;

[0171] Cassia twig mixed reference substance solution: take an appropriate amount of cinnamyl alcohol and cinnamic acid reference substance, weigh them accurately, add methanol to make a solution containing 30 μg of cinnamyl alcohol and 20 μg of cinnamic acid per 1 ml, as the mixed reference substance solution;

[0172] Windproof mixed reference substance solution: take an appropriate amount of reference substances of acteoside, acteoside, 5-O-methyl visamidol glycoside, and hexaquinol glycoside, accurately weigh them, and add methanol to m...

Embodiment 2

[0179] Embodiment 2 Preparation of Guizhi Shaoyao Zhimu Decoction Composition and Preparation of Need Testing Solution

[0180] Weigh the following decoction pieces by weight: Guizhi 12g, Radix Paeoniae Alba 9g, Anemarrhena 12g, Ephedra 6g, Paofupian 6g, Fangfeng 12g, Ginger 15g, Atractylodes Rhizoma Atractylodes Rhizome 5g, Radix Glycyrrhizae 6g; Put Jiuwei decoction pieces in a 5L casserole , add 1400ml of water, soak for 30 minutes, cover, add to boil, keep slightly boiling until the decoction is 400ml, filter it with a 350-mesh filter cloth while it is hot, concentrate the filtrate to an appropriate amount under reduced pressure, freeze-dry, pulverize, and mix well to obtain Composition of Guizhi Shaoyao Zhimu Decoction.

[0181] Take about 0.18g of the above-mentioned Guizhi Shaoyao Zhimu Decoction composition, accurately weigh it, put it in a 10ml measuring bottle, add an appropriate amount of water, and perform ultrasonic treatment for 10-30 minutes (power 250W, frequen...

Embodiment 3

[0182] The investigation of embodiment 3 fingerprint chromatographic conditions

[0183] (1) Selection of detection wavelength

[0184] Use octadecylsilane bonded silica gel as filler; use acetonitrile as mobile phase A and 0.1% phosphoric acid solution as mobile phase B, and carry out gradient elution as specified in the table below; flow rate 0.25ml / ml, column temperature: 30°C.

[0185] Table 1: Mobile Phase Gradient Program for Fingerprinting

[0186]

[0187] Adopt diode array detector to carry out full-wavelength detection to the need testing solution of embodiment 2, according to the three-dimensional graph of collection (see figure 1 ), and simultaneously collect the spectra at 210nm, 230nm, 254nm, 270nm, and 280nm wavelengths (see figure 2 ). Comprehensive investigation, compared with other wavelengths, the composition of Guizhi Shaoyao Zhimu Decoction has the largest peak information at 210nm wavelength, which can characterize the components of ephedrine hydr...

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Abstract

The invention discloses a fingerprint spectrum construction method and a detection method of a Guizhishaoyaozhimu decoction composition, and the fingerprint spectrum construction method comprises the following steps: (1) precisely weighing the Guizhishaoyaozhimu decoction composition, adding water, and carrying out ultrasonic treatment to obtain a test solution; (2) preparing 22 reference substances such as gallic acid, catechin, albiflorin, ephedrine hydrochloride and pseudoephedrine hydrochloride into a reference substance solution; and (3) precisely sucking the test solution and the reference solutions, injecting the test solution and the reference solutions into an ultra-high performance liquid chromatograph for chromatographic analysis at the detection wavelength of 210 nm to obtain a test fingerprint spectrum and a reference chromatographic spectrum, and formulating a standard fingerprint spectrum of the Guizhishaoyaozhimu decoction composition. The quality of a product to be detected can be comprehensively reflected through the standard fingerprint spectrum, and the product quality of the Guizhishaoyaozhimu decoction preparation can be effectively controlled.

Description

technical field [0001] The invention relates to the technical field of quality detection of traditional Chinese medicines, in particular to a fingerprint construction method and a detection method of a composition of Guizhi Shaoyao Zhimu Decoction. Background technique [0002] Guizhi Shaoyao Zhimu Decoction is one of the 100 classic prescriptions in the "Ancient Classic Famous Prescription Catalog (First Batch)" formulated by the State Administration of Traditional Chinese Medicine in conjunction with the State Drug Administration in 2018. From the book "Synopsis of the Golden Chamber" by Zhang Zhongjing, a famous expert in the Eastern Han Dynasty, the prescription is composed of nine herbs of Guizhi, Fuzi (pao), peony, ephedra, ginger, Atractylodes macrocephala, Anemarrhena, Fangfeng and licorice. Among them, cassia twig and aconite are the king medicines, cinnamon twig is pungent, sweet and warm, warms the meridians, benefits the joints and dispels wind pathogens; aconite...

Claims

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Application Information

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IPC IPC(8): G01N30/86
CPCG01N30/8686
Inventor 徐彤彤刘青林碧珊张文芳袁春平魏梅孙冬梅梁浩明曾杉朱德全
Owner SINOPHARM GUANGDONG GLOBAL PHARMA CO LTD
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