Cadmium-reducing biological modified ceramsite and preparation method thereof

A bio-modified, ceramsite technology, used in biochemical equipment and methods, microorganism-based methods, microorganisms, etc.

Active Publication Date: 2021-07-13
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The problem to be solved by the present invention is to provide a cadmium-reducing bio-modified ceramsite and its preparation method. The cadmium-reducing b

Method used

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  • Cadmium-reducing biological modified ceramsite and preparation method thereof
  • Cadmium-reducing biological modified ceramsite and preparation method thereof
  • Cadmium-reducing biological modified ceramsite and preparation method thereof

Examples

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Embodiment 1

[0029] Embodiment 1, a kind of biological ceramsite that adsorbs cadmium function, its preparation method is to carry out the following steps successively:

[0030] 1), weigh 1200g ceramsite (particle size is 3 ~ 5mm), soak ceramsite in 2% (mass %) glutaraldehyde solution for 2 ~ 4h, wash 3 times with deionized water, high temperature sterilization (in Sterilize at 121°C for 20 minutes), and dry at 25°C for 6 hours to obtain sterile ceramsite.

[0031] Note: During the whole soaking process, the ceramsite must always be submerged by 2% glutaraldehyde solution.

[0032] 2), in 250mL Erlenmeyer flask, add 100mL beef extract peptone medium (for conventional medium), 1% (volume %) inoculum amount inserts cadmium-resistant bacterial strain Bacillus sp.k1, in 25 ℃, 180rpm constant temperature shaker Cultivate to the logarithmic phase, adjust OD=1 with a UV spectrophotometer (600nm), and prepare bacterial suspension I (≥1×10 8 CFU / mL).

[0033] Centrifuge the bacterial suspension ...

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Abstract

The invention discloses cadmium-reducing biological modified ceramsite and a preparation method thereof, and the preparation method comprises the following steps: soaking ceramsite in a 1.5-2.5% glutaraldehyde solution, washing with water, sterilizing, and drying to obtain sterile ceramsite; preparing a bacterial suspension I of a cadmium-resistant functional strain with the concentration of more than or equal to 1*10 <8> CFU/mL, centrifuging the bacterial suspension I, discarding a supernate, and adding normal saline into a thallus precipitate to re-suspend the thallus to form a bacterial suspension II with the OD of 0.9-1.1; mixing the sterile ceramsite with the bacterial suspension II, oscillating and drying; mixing the dried ceramsite with the bacterial suspension II again, shaking, and drying; and obtaining the cadmium-reducing biological modified ceramsite. The method can be used for treating the cadmium pollution problem in the water body, and meanwhile has the potential of repairing the cadmium pollution problem in the soil.

Description

technical field [0001] The invention relates to a biologically modified ceramsite for reducing cadmium and a preparation method thereof. Background technique [0002] On the journey of continuous development of my country's modern industry and agriculture, for a long period of time, domestic enterprises have not paid enough attention to environmental protection. Among them, the industrial "three wastes" that have not reached the standard are discharged in large quantities, the mining and smelting of metal mines, and sewage irrigation A large number of domestic water bodies and soils have suffered severe heavy metal pollution due to factors such as these. Among them, the pollution of heavy metal cadmium (Cd) is particularly serious. Cd was designated as a key environmental pollutant by the United Nations Environmental Planning Bureau and the International Labor Health Heavy Metal Committee in 1974. It is easy to accumulate in the human body. When it is ingested by the human bo...

Claims

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Application Information

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IPC IPC(8): C12N11/14B01J20/02B01J20/30C02F1/28C02F101/20
CPCC12N11/14B01J20/02C02F1/281C02F2101/20C12R2001/07C12N1/20C02F1/288B09C1/08B01J20/12B01J20/28016B01J20/22B01J2220/4868
Inventor 张奇春张耿苗狄蕊张祝宁李厚孚
Owner ZHEJIANG UNIV
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