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Alkali-extracted heteropolysaccharide from fruit body of Cordyceps militaris and its preparation method and application

A technology for polysaccharide and grass seed, applied in the field of biologically active substances and their preparation, can solve the problems of wide range, complex composition, unfavorable polysaccharide structure-activity relationship and the like

Active Publication Date: 2022-04-15
WEIFANG MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, most of the Cordyceps militaris polysaccharides reported in the literature use the method of water extraction and alcohol precipitation, but although the process of obtaining polysaccharides by alcohol precipitation is simple, the obtained polysaccharides have a wide range of relative molecular weight and complex composition, which is not conducive to the structure-efficiency of polysaccharides. The elaboration of the relationship is also not conducive to product quality control and subsequent clinical transformation applications
[0007] In recent years, researchers at home and abroad have reported the lipid-lowering and anti-atherosclerotic effects of Cordyceps militaris extract alone or in combination, and there are also reports on the extraction of polysaccharides from Cordyceps militaris by water; There are few reports in the literature, and the structure and preparation method of alkali-extracted polysaccharides with anti-atherosclerosis and lipid-lowering functions have not been reported

Method used

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  • Alkali-extracted heteropolysaccharide from fruit body of Cordyceps militaris and its preparation method and application
  • Alkali-extracted heteropolysaccharide from fruit body of Cordyceps militaris and its preparation method and application
  • Alkali-extracted heteropolysaccharide from fruit body of Cordyceps militaris and its preparation method and application

Examples

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Effect test

Embodiment 1

[0026] Example 1: Preparation of Alkali-extracted Heteropolysaccharide CM3-W from Fruiting Body of Cordyceps Militaris and Its Anti-Atherosclerosis Application

[0027] When extracting and preparing the alkali-extracted heteropolysaccharide CM3-W of the Cordyceps militaris fruiting body of the present invention and verifying its lipid-lowering and anti-atherosclerosis effects on the apolipoprotein E knockout mouse model, the following steps were taken:

[0028] 1) Preparation of crude polysaccharides by alkaline extraction of fruiting body of Cordyceps militaris: fruiting body of Cordyceps militaris, dried in the shade, pulverized with a plant grinder, and passed through a 100-mesh sieve. Add 95% ethanol to the fruiting body of Cordyceps militaris after breaking the wall and reflux for 2 hours, the ratio of solid to liquid is 1:6 (W / V), extract the defatted and soluble molecules, and filter to obtain solid matter. Distilled water was added to the solid, the ratio of solid to l...

Embodiment 2

[0037] Example 2: Preparation of Alkaline Extracted Heteropolysaccharide CM3-W from Fruiting Body of Cordyceps militaris and Its Anti-Atherosclerosis Application

[0038] When extracting and preparing the alkali-extracted heteropolysaccharide CM3-W from Cordyceps militaris fruiting body of the present invention and verifying its lipid-lowering and anti-atherosclerotic activity on the low-density lipoprotein receptor knockout (LDLR- / -) mouse model , the following steps are taken:

[0039] 1) Preparation of crude polysaccharides by alkaline extraction of fruiting body of Cordyceps militaris: fruiting body of Cordyceps militaris, dried in the shade, pulverized with a plant grinder, and passed through a 200-mesh sieve. Add 95% ethanol to the broken Cordyceps militaris fruit body and reflux for 2.5 hours, the ratio of solid to liquid is 1:8 (W / V), extract the defatted and soluble molecules, and filter to obtain solid matter. Add distilled water to the solid, the ratio of solid to ...

Embodiment 3

[0052] Example 3: Preparation of Heteropolysaccharide CM3-W Alkali Extracted from Fruiting Body of Cordyceps militaris and Its Lipid-lowering Application

[0053] When extracting and preparing the alkali-extracted heteropolysaccharide CM3-W of the Cordyceps militaris fruiting body of the present invention and verifying its lipid-lowering activity on the high-fat-induced hamster model, the following steps were taken:

[0054] 1) Preparation of crude polysaccharides by alkaline extraction of fruiting bodies of Cordyceps militaris: fruiting bodies of Cordyceps militaris were dried overnight in an oven at 50°C, pulverized with a plant grinder, and passed through a 100-mesh sieve. Add 95% ethanol to the fruiting body of Cordyceps militaris after breaking the wall and reflux for 3 hours, the ratio of solid to liquid is 1:6 (W / V), extract the defatted and soluble molecules, and filter to obtain solid matter. Add distilled water to the solid, the ratio of solid to liquid is 1:12 (W / V)...

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Abstract

The invention discloses an alkali-extracted heteropolysaccharide from the fruit body of Cordyceps militaris and its preparation method and application. Polysaccharides can be used to prepare drugs to reduce plasma total cholesterol, triglyceride and low-density lipoprotein cholesterol levels, increase plasma lipoprotein esterase activity, and increase the expression of liver X receptor α (LXRα).

Description

technical field [0001] The invention relates to a biologically active substance and its preparation method and application, in particular to an alkali-extracted heteropolysaccharide from the fruit body of Cordyceps militaris, its preparation method and its application for reducing lipid. Background technique [0002] At present, cardiovascular and cerebrovascular diseases are diseases with higher morbidity and mortality than tumors, and are the number one disease threatening human health. The main pathological process is that on the basis of vascular wall lesions, denatured low-density lipoprotein (LDL) and other components gradually deposit under the intima of the blood vessels, resulting in ischemic or hemorrhagic diseases. Atherosclerosis is the basic lesion of cardiovascular disease. [0003] Hyperlipidemia is one of the main factors inducing atherosclerosis. When cholesterol in the body is too high, especially when the level of low-density lipoprotein (LDL-C) and trig...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61K31/715A61K36/068A61P3/06
CPCC08B37/0003C08B37/006A61K31/715A61K36/068A61P3/06
Inventor 郭守东尹凡沈诺
Owner WEIFANG MEDICAL UNIV
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