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Bacillus velesi ljbv19 and its application

A technology of LJBV19 and Bacillus, applied in the field of microorganisms, can solve the problems of soil microbial richness and soil pH reduction, quality and yield decline, pathogenic bacteria resistance, etc., and achieve the effects of improving fruit quality, developing agricultural economy, and promoting growth

Active Publication Date: 2022-07-29
SHANGHAI JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Frequent use of chemical pesticides in large quantities may easily cause environmental pollution and pesticide residue problems, and also easily lead to drug resistance of pathogenic bacteria
The long-term excessive use of chemical fertilizers leads to the reduction of soil microbial richness and soil pH, making crops unable to obtain these fertilizers, thereby reducing crop quality and yield, and directly or indirectly causing environmental pollution

Method used

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  • Bacillus velesi ljbv19 and its application
  • Bacillus velesi ljbv19 and its application
  • Bacillus velesi ljbv19 and its application

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0033] (1) Preparation of test medium

[0034] 1) PDA medium: 200 g of potato, 20 g of glucose, 15 g of agar powder, 1000 mL of distilled water, sterilized by high pressure steam at 121° C. for 20 min.

[0035]2) LB liquid medium: 10 g of tryptone, 5 g of yeast extract, 10 g of sodium chloride, 1000 mL of distilled water, adjusted to pH 7.0, and autoclaved at 121° C. for 20 min. For LB solid medium, add 15g agar powder per liter of distilled water.

[0036] 3) Citrate medium: NaCl 5.0 g, MgSO 4 ·7H 2 O 0.2g, NH 4 H 2 PO 4 1.0g, K 2 HPO 4 1.0g, sodium citrate 2.0g, 0.04% phenol red 10mL, agar 15g, distilled water 1000mL.

[0037] 4) Starch agar medium: beef extract 2.0g, protein 17.0g, agar 15.0g, soluble starch 2.0g, distilled water 1000mL.

[0038] 5) Gelatin medium: 5.0 g of peptone, 120.0 g of gelatin, 3.0 g of beef extract, and 1000 mL of distilled water.

[0039] (2) Cultivation of tested plant pathogenic bacteria strains

[0040] Rice blast fungus (M.oryzae)...

Embodiment 1

[0041] Example 1: Isolation and screening of strains

[0042] (1) Isolation of strains

[0043] The soil was taken from the grape rhizosphere, and the sampling requirements were within 20cm below the surface, because the grape roots were mainly distributed in this piece. Soak the soil with physiological saline (m:v=1:10), shake it evenly, and let it stand for 30 minutes. The supernatant after the warm bath was diluted 1, 10, 100, and 1000 times and then spread on LB solid medium, cultured at 37°C for 1 day, and single bacterial plaque with milky white color and biofilm was selected for streak preservation. The 16S rRNA gene was amplified with 27F / 1492R primer, sequenced and aligned, and a single colony belonging to Bacillus was retained. A single colony was inoculated into LB liquid medium, and the bacterial suspension was obtained by shaking culture on a shaker at 37°C and 180rpm for 24h. The bacterial suspension was mixed with 50% sterile glycerol in equal proportions and...

Embodiment 2

[0047] Example 2: Identification of strain LJBV19

[0048] (1) Physiological and biochemical characteristics of strain LJBV19

[0049] Observation of colony morphology: Pick an appropriate amount of bacterial lawn that has been cultured for 24 hours, inoculate it in LB liquid medium, and culture with shaking on a shaker at 37°C and 180 rpm for 48 hours. Take 3 μL drop on the plate, mainly record the colony shape, size, edge, surface, bump shape, transparency, and color.

[0050] Gram staining: Pick an appropriate amount of bacterial moss cultured for 24 hours and spread it evenly on a clean glass slide with a drop of distilled water in the center, air-dry, and fix the smear several times over the flame, then stain with crystal violet for 1 min; After rinsing, iodine solution was added dropwise for 1 min; after rinsing with running water, decolorized with 95% alcohol for 30 s; after rinsing with running water, counterstained with safranin for 2-3 min; after rinsing with runnin...

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Abstract

The invention discloses a strain of Bacillus velesi LJBV19 isolated from grape rhizosphere soil. February 2, 2021; The present invention also discloses a broad-spectrum disease-preventing and growth-promoting fungicide and its application in preventing and treating plant diseases caused by plant pathogens. The Bacillus velesi LJBV19 of the present invention has a wider spectrum of disease prevention and growth promotion effects, and can not only effectively control plant diseases caused by rice pathogens, grape pathogens, Solanaceae crop pathogens, and forest tree pathogens, wherein, Bacillus LJBV19 has an average inhibition rate of 75.75% against rice blast fungus; it can also promote plant growth, increase crop yield, improve fruit quality, improve crop resistance, develop agricultural economy, and promote agricultural development.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a Bacillus velesi LJBV19 and its application. Background technique [0002] In order to increase the income and yield of crops, chemical pesticides and chemical fertilizers have been widely used in agricultural production. The frequent and large-scale use of chemical pesticides is easy to cause environmental pollution and pesticide residue problems, and it is also easy to cause pathogenic bacteria to develop drug resistance. The long-term excessive use of chemical fertilizers leads to the decrease of soil microbial richness and soil pH, making it impossible for crops to obtain these fertilizers, thereby reducing the quality and yield of crops, and directly or indirectly causing environmental pollution. Therefore, it is particularly important to develop biofertilizers and biopesticides that are environmentally friendly, improve soil fertility, enhance plant resistance, and...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/22A01P3/00A01P21/00C12R1/07
CPCA01N63/22
Inventor 傅佩宁卢江汪博彭行吴伟彭亚纯
Owner SHANGHAI JIAOTONG UNIV
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