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Method for separating cell culture mixture

A cell culture and cell separation technology, applied in biochemical equipment and methods, animal cells, reproductive tract cells, etc., can solve the problems of shear force sensitivity, cell fragmentation, and reduced reusability of cell phases.

Active Publication Date: 2021-07-23
ALFA LAVAL CORP AB
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, handling cell culture mixtures such as cell culture mixtures from fermentation broths can lead to excessive fragmentation of the cells, as for example mammalian cells and CHO cells can be sensitive to the shear forces experienced in the centrifugal field
This consequently results in a reduced reusability of the isolated cell phase

Method used

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  • Method for separating cell culture mixture
  • Method for separating cell culture mixture
  • Method for separating cell culture mixture

Examples

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Embodiment Construction

[0070] In the foregoing, the inventive concept has mainly been described with reference to a limited number of examples. However, as is readily appreciated by a person skilled in the art, other examples than the ones disclosed above are equally possible within the scope of the inventive concept defined by the appended claims.

[0071] as below reference Image 6 As discussed, the method 50 for isolating a cell culture mixture can be utilized with reference to Figure 1 to Figure 4 The centrifugal separator in question.

[0072] figure 1 Shown is an external side view of a rotatable member in the form of a replaceable separation insert 1, which may be used in the centrifugal separator of the present disclosure.

[0073] As seen in the axial direction defined by the axis of rotation (X), the insert 1 comprises a rotor housing 2 arranged between a first lower fixing part 3 and a second upper fixing part 4 . The first fixing part 3 is at the lower axial end 5 of the insert 1 ,...

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Abstract

The present invention provides a method for separating a cell culture mixture. A centrifugal separator comprising a stationary frame (30), a rotatable assembly (101) and a drive unit (34) for rotating the rotatable assembly (101) relative the frame (30) around a vertical axis of rotation (X) is utilised. The rotatable assembly (101) comprises a rotor casing (2) enclosing a separation space (17) in which a stack (19) of separation discs is arranged to rotate around an axis (X) of rotation; said rotor casing (2) further comprising a mechanically hermetically sealed inlet (20) for supply of said cell culture mixture to said separation space (17) and first and second mechanically hermetically sealed liquid outlets (21, 22) The said second mechanically hermetically sealed outlet (22) is arranged at an axial end (6) of said rotor casing (2) and arranged so that separated cell phase is discharged at the rotational axis (X).

Description

technical field [0001] The inventive concept relates to the field of centrifugal separators. More specifically, it relates to a method for separating cell culture mixtures. Background technique [0002] Centrifugal separators are commonly used to separate liquids and / or solids from liquid mixtures or gas mixtures. During operation, the fluid mixture to be separated is introduced into the rotating bowl, and due to centrifugal force, heavy particles or denser liquids such as water collect at the periphery of the rotating bowl, while less dense liquids collect closer to the center axis of rotation. This allows collecting the separated fractions eg by means of different outlets arranged at the periphery and close to the axis of rotation, respectively. [0003] WO 2015 / 181177 discloses a separator for centrifugation of pharmaceutical products such as fermentation broths. The separator includes a rotatable outer drum and a replaceable inner drum arranged in the outer drum. Th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B04B1/08B04B11/02B04B7/00
CPCB04B1/08B04B7/00B04B11/02B04B7/14B04B9/12C12M47/02C12N5/0682
Inventor P·托尔韦德K·霍格隆德
Owner ALFA LAVAL CORP AB