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Method for producing sugarcane virus-free seedlings

A production method and technology of virus-free seedlings, applied in horticultural methods, botany equipment and methods, plant regeneration, etc., can solve the problems of restricting the popularization and application of virus-free seedlings, poor heat resistance, etc., and achieve fast start-up of micropropagation and low cost Inexpensive, fast growing effect

Active Publication Date: 2021-07-27
广东省科学院南繁种业研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, fruit cane is widely used in sugarcane detoxification seedlings, and black-skinned fruit cane accounts for more than 75% of the fruit cane planting area, but the heat resistance of black-skinned fruit cane is poor, which restricts the virus-free seedlings Promotion and application in production
However, there is no research report on how to protect sugarcane buds from hot water damage

Method used

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  • Method for producing sugarcane virus-free seedlings
  • Method for producing sugarcane virus-free seedlings
  • Method for producing sugarcane virus-free seedlings

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1: detoxification treatment of yellow-skin fruit cane

[0038](1) Select the yellow-skinned fruit cane plants that are free from diseases and insect pests and grow vigorously, select the plump buds on the upper part, cut the cane stems into cane seeds with single buds, soak them in water for 12 hours, rinse them, pick them up and dry them in the air.

[0039] (2) Place 3 layers of filter paper on the surface of the cane buds, tear off the anti-adhesive film of the leak-proof stickers, stick the leak-proof stickers on the filter paper covering the cane buds and the surrounding sugarcane stems, and press the edges tightly to prevent The central position covered by the leak-proof sticker is the cane bud; among them, the area of ​​the filter paper placed is 0.5-0.8×0.8-1.5cm, whichever can completely cover the cane bud; the area of ​​the leak-proof sticker is 2.0-2.5× 2.5-3.0cm, the area is larger than the filter paper, and each side of the leak-proof sticker is ...

Embodiment 2

[0045] Embodiment 2: detoxification treatment of black fruit cane (Badila)

[0046] (1) Select the black-skinned fruit cane (Badila) plants that are free from diseases and insect pests and grow vigorously, select the plump buds on the upper part, cut the cane stems into cane seeds with single buds, soak them in water for 16 hours, rinse them, pick them up and dry them in the air.

[0047] (2) Place 5 layers of filter paper on the surface of the cane buds, after tearing off the anti-adhesive film of the leak-proof stickers, stick the leak-proof stickers on the filter paper covering the cane buds and the surrounding sugarcane stems, and press the edges tightly to prevent The central position covered by the leak-proof sticker is the cane bud; among them, the area of ​​the filter paper placed is 0.5-0.8×0.8-1.5cm, whichever can completely cover the cane bud; the area of ​​the leak-proof sticker is 2.0-2.5× 2.5-3.0cm, the area is larger than the filter paper, and each side of the l...

Embodiment 3

[0052] Example 3: Detoxification treatment of Yuetang 93-159

[0053] (1) Choose the vigorously growing Yuetang 93-159 plant without pests and diseases, select the plump buds on the upper part, cut the cane stems into cane seeds with single buds, soak them in water for 10 hours, rinse them, pick them up and dry them in the air.

[0054] (2) Place 5 layers of filter paper on the surface of the cane buds, after tearing off the anti-adhesive film of the leak-proof stickers, stick the leak-proof stickers on the filter paper covering the cane buds and the surrounding sugarcane stems, and press the edges tightly to prevent The central position covered by the leak-proof sticker is the cane bud; among them, the area of ​​the filter paper placed is 0.5-0.8×0.8-1.5cm, whichever can completely cover the cane bud; the area of ​​the leak-proof sticker is 2.0-2.5× 2.5-3.0cm, the area is larger than the filter paper, and each side of the leak-proof sticker is 0.5-0.7cm wider than the filter ...

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Abstract

The invention discloses a method for producing sugarcane virus-free seedlings. According to the method disclosed by the invention, when the sugarcane seeds are treated by hot water, a leakage-proof adhesive tape used on a building is used for sticking and sealing sugarcane buds, so that the sugarcane buds can be well protected from being damaged by the hot water, the sugarcane buds rapidly germinate and vigorously grow, effective killing of pathogenic microorganisms in sugarcane stems by the hot water is not influenced, microorganisms at two ends of the sugarcane stems and in a germination accelerating environment are inhibited or the breeding speed is greatly slowed down; normal and pollution-free growing point explants can be easily obtained by separation, micro-propagation starting is fast, cluster buds can be rapidly formed, the propagation coefficient is high, operation is easy, practicability is high, cost is low, and test materials are saved. The method plays an important role in promoting the application of healthy seedlings in production and promoting the sustainable development of the cane sugar industry.

Description

technical field [0001] The invention belongs to the technical field of plant seedling tissue culture rapid propagation, and in particular relates to a method for producing poison-free sugarcane seedlings. Background technique [0002] Sugarcane is the most important sugar crop in China. In terms of production, the sugarcane shoots kept during harvest are usually used as seeds, and the seed buds are used as propagules for asexual reproduction. After planting for many years, due to repeated infection and accumulation of various pathogens in the plants, the accumulation of disease pathogens becomes more and more serious. More and more serious, such as sugarcane perennial dwarf disease, mosaic disease, white streak disease, pineapple disease, smut, downy mildew, virus disease, etc. are all transmitted through sugarcane stem seedlings, which seriously lead to continuous decline in quality and output. The phenomenon of variety degeneration has caused serious economic losses to su...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 劳方业何慧怡齐永文
Owner 广东省科学院南繁种业研究所
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