Cellulase system suitable for improving corn fiber sugar conversion rate in saccharification liquid preparation and application of cellulase system

A corn fiber and cellulase technology, applied in the directions of glycosylase, biochemical equipment and methods, enzymes, etc., can solve the problems of low efficiency, high energy consumption, and inhibitors affecting the reaction, etc., and achieves simple preparation process and hydrolysis. The effect of high efficiency and low equipment requirements

Pending Publication Date: 2021-07-27
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, patent 201911209469.5 uses ultrasonic-assisted hydrochloric acid to hydrolyze corn fiber. After microbial hydrolysis, corn fiber hydrolyzate is obtained by concentration and steam treatment. Compared with unpretreated corn fiber, the total sugar concentration in the hydrolyzate has a certain increase, but in the process High energy consumption, and at the same time, inhibitors will be produced to affect subsequent reactions
[0006] The search found that there is still a lack of special enzyme products that can effectively hydrolyze corn fiber on the market. The reported cellulase used in the enzymatic conversion of corn fiber to sugar production is still low. Research report on enzyme system or cellulase system suitable for improving the conversion rate of corn fiber sugar in the preparation of saccharification liquid and its application

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1 Preparation of a cellulase system suitable for improving the conversion rate of corn fiber sugar in the preparation of saccharification liquid

[0045] The composite cellulase system is composed of a Trichoderma reesei enzyme system with a filter paper enzyme activity of not less than 5 FPU / mL, a xylanase with an enzyme activity of not less than 500 U / mL, and arabic acid with an enzyme activity of not less than 50 U / mL. The furanosidase is prepared according to the volume ratio of 1000:185:35.

[0046] In the composite cellulase system, the filter paper enzyme activity of the Trichoderma reesei enzyme system is 15 FPU / mL, the xylanase activity is 900 U / mL, and the arabinofuranosidase activity is 3 U / mL.

Embodiment 2

[0047] Example 2 Preparation of a cellulase system suitable for improving the conversion rate of corn fiber sugar in the preparation of saccharification liquid

[0048] The composite cellulase system is composed of a Trichoderma reesei enzyme system with a filter paper enzyme activity of not less than 5 FPU / mL, a xylanase with an enzyme activity of not less than 500 U / mL, and arabic acid with an enzyme activity of not less than 50 U / mL. Furanosidase and acetylxylan esterase with an enzyme activity of not less than 100U / mL are prepared according to the volume ratio of 1000:185:35:14.

[0049] In the composite cellulase system, the filter paper enzyme activity of the Trichoderma reesei enzyme system is 15FPU / mL, the xylanase activity is 900U / mL, the arabinofuranosidase activity is 3U / mL, and the acetylxylan esterase enzyme activity is 3U / mL. The activity is 0.75U / mL.

Embodiment 3

[0050] Example 3 Preparation of cellulase system suitable for improving the conversion rate of corn fiber sugar in the preparation of saccharification liquid

[0051] The composite cellulase system is composed of a Trichoderma reesei enzyme system with a filter paper enzyme activity of not less than 5 FPU / mL, a xylanase with an enzyme activity of not less than 500 U / mL, and arabic acid with an enzyme activity of not less than 50 U / mL. Furanosidase and feruloesterase with an enzyme activity of not less than 5U / mL are prepared according to the volume ratio of 1000:185:35:7.

[0052] In the composite cellulase system, the filter paper enzyme activity of the Trichoderma reesei enzyme system is 15FPU / mL, the xylanase activity is 1000U / mL, the arabinofuranosidase activity is 3U / mL, and the ferulic acid esterase activity is 0.3U / mL.

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Abstract

The invention discloses a cellulase system suitable for improving corn fiber sugar conversion rate in saccharification liquid preparation, which is a composite cellulase system prepared by taking a trichoderma reesei enzyme system as a main enzyme system and adding xylanase and arabinofuranosidase; or one or two of acetylxylan esterase and feruloyl esterase is / are further added on the basis to prepare the composite cellulase system; wherein the compound cellulase system is prepared from a trichoderma reesei enzyme system of which the filter paper enzyme activity is not lower than 5FPU / mL, xylanase of which the enzyme activity is not lower than 500U / mL, arabinofuranosidase of which the enzyme activity is not lower than 50U / mL, acetyl xylan esterase of which the enzyme activity is not lower than 50U / mL and feruloyl esterase of which the enzyme activity is not lower than 5U / mL according to a volume ratio of 1000: (100-200): (20-40): (0-20): (0-10). Compared with a starting enzyme system, the corn fiber sugar conversion rate and the ethanol yield per unit corn are improved, efficient conversion from corn to fuel ethanol is achieved, meanwhile, vinasse protein obtained after ethanol distillation is greatly improved, and processing of high-quality feed is facilitated.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a cellulase system suitable for improving the conversion rate of corn fiber sugar in the preparation of saccharification liquid and its application. Background technique [0002] Fuel ethanol refers to the ethanol that can be used as fuel obtained from biomass as raw material through biological fermentation and other ways. The first generation of fuel ethanol is produced with sugary and starchy crops as raw materials, which has the disadvantage of "competing with others for food", and the production scale and development are limited. The second-generation fuel ethanol is based on renewable lignocellulosic biomass, which is in line with the concept of sustainable development and is a development trend. The production of fuel ethanol from lignocellulose generally includes three steps: raw material pretreatment, enzymatic saccharification, and ethanol fermentation. However, at present, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/18C12N9/24C12N9/42C12P7/10C12P19/02C12P19/12C12P19/14
CPCC12N9/2437C12N9/248C12N9/2402C12N9/18C12Y302/01055C12Y301/01072C12Y301/01073C12P19/02C12P19/12C12P19/14C12P7/10Y02E50/10
Inventor 李雪芝郭英杰赵建
Owner SHANDONG UNIV
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