A kind of cellulase suitable for preparing cellulose nanocrystal and its application

A technology of cellulase and nanocrystals, which is applied in the biological field to achieve the effects of small particle size, high hydrolysis efficiency, and accelerated fracture
CN111019924BActive Publication Date: 2022-05-03SHANDONG UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
SHANDONG UNIV
Publication Date
2022-05-03

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Abstract

The invention discloses a cellulase suitable for preparing cellulose nanocrystals and an application thereof, wherein the cellulase is prepared by the following steps: (1) construction of an engineering strain; (2) production of cellulase by liquid fermentation . In the process of hydrolyzing cellulose, the cellulase of the present invention strengthens the enzyme component that degrades the amorphous region of cellulose and the protein component that depolymerizes the hydrogen bonds in the large-sized cellulose crystal region, thereby promoting the formation of cellulose macromolecular chains. Fragmentation and "peeling" reaction of cellulose crystallization area, so it is easier to form nanoscale cellulose crystals by using the cellulase of the present invention. Compared with the existing commercial cellulase, the enzyme system composition of the cellulase of the present invention is more suitable for the preparation of cellulose nanocrystals, and has the advantages of controllable enzyme system, high hydrolysis efficiency, low enzyme cost, simple preparation process, It has the advantages of not needing to carry out mechanical treatment on the raw material, and the yield of nanocellulose is high.
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Description

technical field

[0001] The invention relates to a cellulase and an application thereof, in particular to a cellulase suitable for preparing cellulose nanocrystals and an application thereof, and belongs to the field of biotechnology. Background technique

[0002] Cellulose nanocrystals have attracted extensive attention in recent years due to their renewable, nontoxic, high specific surface area, excellent mechanical properties, and easy functionalization of hydroxyl groups. Cellulose nanocrystals have the potential to develop a variety of products that are expected to replace traditional petrochemical and non-biodegradable polymer materials, including food packaging, films, and nanocellulose silver-loaded agents with antibacterial properties.

[0003] The preparation methods of cellulose nanocrystals include concentrated acid hydrolysis and biological methods. Among them, the hydrolysis of concentrated sulfuric acid is the most commonly used preparation method at present, ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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