Atractylodes polysaccharide anti-alcoholic liver injury and its preparation method and application

A technology of Atractylodes macrocephala polysaccharides and liver damage, applied in the direction of resisting vector-borne diseases, medical preparations containing active ingredients, and pharmaceutical formulas, can solve the problems of not seeing the protective effect of Atractylodes macrocephala polysaccharides, and achieve SOD and GSH-Px activity The effect of reducing and alleviating liver damage and inhibiting the increase of liver index

Active Publication Date: 2022-06-07
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Literature research found that there was no research on the protective effect of Atractylodes macrocephala polysaccharide on alcoholic liver injury

Method used

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  • Atractylodes polysaccharide anti-alcoholic liver injury and its preparation method and application
  • Atractylodes polysaccharide anti-alcoholic liver injury and its preparation method and application
  • Atractylodes polysaccharide anti-alcoholic liver injury and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1 The preparation method of Atractylodes polysaccharide

[0037] 1. Extraction of Atractylodes Rhizoma Crude Polysaccharide:

[0038] Take the medicinal materials of Atractylodes Rhizoma, pulverize, add 15 times the amount of water to extract 3 times, each time for 1 hour, combine the extracts, concentrate to obtain a concentrated solution, add ethanol to make the final concentration of ethanol in the system to 80%, set aside for precipitation, filter or centrifuge, Drying to obtain Atractylodes Rhizoma crude polysaccharide;

[0039] 2. Decolorization of macroporous resin:

[0040] Take step (1) Atractylodes Rhizoma crude polysaccharide, add water to dissolve, obtain Atractylodes Rhizoma crude polysaccharide solution with a concentration of 25mg / mL, adjust the pH value of the polysaccharide solution to 6, add HPD600 macroporous resin column, place in a shaking table, 125r / min, at temperature Decolorize at 75°C, decolorize for 6h, centrifuge at 3000r / min for 3...

Embodiment 2

[0054] Example 2 Structure identification of Atractylodes polysaccharide

[0055] 1. Purity identification and relative molecular mass determination

[0056] 1. Materials and methods

[0057] 1.1 Materials and Instruments

[0058] Atractylodes polysaccharide (BZJP) prepared in Example 1, dextran molecular weight standard set (10 kinds), including dextran DO Mp180, dextran D1 Mp2700, dextran D2 Mp5250, dextran D3 Mp9750, dextran D4Mp13050, dextran D5 Mp36800, dextran D6 Mp64650, D7 Mp135350, dextran D8Mp300600, dextran D2000 Mp2000000 (China Institute for Food and Drug Control); Coomassie brilliant blue G-250, bovine serum albumin, phenol, sulfuric acid, phosphoric acid, etc. are all domestic analytical grades.

[0059] Model 101-00B electric heating constant temperature drying oven (Luyue Instrument Equipment Factory, Shangyu District, Shaoxing City); Agilent 1260 High Performance Liquid Chromatograph (Agilent Technology Co., Ltd.).

[0060] 1.2 Experimental method

[0061...

Embodiment 3

[0115] Example 3 Study on the protective effect of Atractylodes polysaccharide on acute alcoholic liver injury in mice

[0116] 1. Materials and methods

[0117] 1.1 Materials and Instruments

[0118] 1.1.1 Experimental animals:

[0119] Clean grade Kunming mice, male, weighing (20±2) g, were purchased from Qinglong Mountain. The temperature of the rearing environment was (23±2)°C, the humidity was 40%-60%, and the light-dark cycle was 12h.

[0120] 1.1.2 Drugs and Reagents

[0121] Atractylodes polysaccharide BZTP (prepared in Example 1); absolute ethanol (Sinopharm Group Co., Ltd.); aspartate aminotransferase (AST), alanine aminotransferase (ALT), triglyceride (TG), Malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), total protein quantitative determination (BCA method) kit (Nanjing Jiancheng Bioengineering Institute).

[0122] 1.1.3 Instruments

[0123] Tissuelyser-48 automatic sample rapid grinder (Shanghai Jingxin Technology); multi-f...

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Abstract

The invention discloses an anti-alcoholic liver injury Atractylodes macrocephala polysaccharide and a preparation method thereof. The Atractylodes macrocephala polysaccharide is composed of glucose, mannose and xylose in a molar ratio of 6.8:3.0:1.0; its molecular weight is 5465 Da; The secondary structure is: βXyl p ‑[(1→3)‑βXyl p ] 6 -(1→6)-αGlc p ‑[(1→4)‑αGlc p ] 40 ‑[(1→2)‑αMan p ] 12 . The experimental results show that: Atractylodes macrocephala polysaccharide has obvious protective effect on acute alcoholic liver injury in mice, can significantly inhibit the increase of serum AST, ALT and TG activities caused by alcohol acute liver injury, and effectively inhibit the alcohol intake-induced The increase of liver index, the increase of MDA content in the liver, and the decrease of SOD and GSH‑Px activities can effectively relieve the symptoms of alcoholic liver injury in mice, which shows that Atractylodes macrocephala polysaccharides have a better effect on alcohol-induced acute liver injury in mice. Good protection.

Description

technical field [0001] The invention relates to a plant polysaccharide, in particular to an Atractylodes macrocephala polysaccharide resistant to alcoholic liver injury and a preparation method thereof. Background technique [0002] Alcoholic liver disease (Alcoholic Hepatitis) is a liver disease caused by long-term heavy drinking. The initial stage usually manifests as fatty liver, which can progress to alcoholic hepatitis, liver fibrosis, cirrhosis, and even liver cancer. The morbidity and mortality of alcoholic liver disease are increasing year by year, and it has become a global public health problem. At present, there are about 2.5 million deaths due to alcohol poisoning in the world every year, accounting for 4% of the total mortality, surpassing AIDS as the eighth leading cause of death, and alcohol has become the second leading cause of liver damage after hepatitis virus. It brings a heavy economic burden to individuals, families and society. A nationwide survey o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61K31/715A61P1/16
CPCC08B37/006C08B37/0003A61K31/715A61P1/16Y02A50/30
Inventor 王令充李俊松狄留庆杨颖嵇晶
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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