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Molecular marker and application of kasp for identification of soybean wrinkle resistance

A technology of molecular markers and resistance identification, which is applied in the field of agricultural biology, can solve the problems of time-consuming and other problems, and achieve the effects of high accuracy of resistance identification, high detection success rate and low price

Active Publication Date: 2022-07-12
GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Since leaf folds are affected by the environment, and the traits are not manifested until mid-growth, relying solely on phenotypic identification is not only time-consuming, but also has certain limitations.

Method used

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  • Molecular marker and application of kasp for identification of soybean wrinkle resistance
  • Molecular marker and application of kasp for identification of soybean wrinkle resistance
  • Molecular marker and application of kasp for identification of soybean wrinkle resistance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1) Genetic population construction and field identification of wrinkle resistance: Using two materials with large differences in wrinkle resistance, Guichun No. 8 (normal) and Y2017-1 (wrinkle grade 4) were crossed, and obtained after removing false hybrids F1 single plant. F1 individual plant seeds were planted in rows, and F2 individual plants and young leaf samples of F2 individual plants were obtained. The DNA of the samples was extracted by DNA extraction kits and stored according to the numbers. Also refer to Chen Wenjie et al. (2020) (Chen Wenjie, Chen Yuan, Wei Qingyuan, Guo Xiaohong, Tang Fuyue, Yang Meng, Ye Wandian, Liang Jiang. Occurrence characteristics and material disease grade identification of a soybean wrinkle disease [J]. Soybean Science, 2020, 39(3)) Identification of Wrinkled Leaf Disease in F2 Individual Plants class. The F2:3 seed row of F2 single plant was planted, and the homozygous heterozygous condition of wrinkled leaf of each row was identi...

Embodiment 2

[0030] Using the molecular marker of soybean wrinkle resistance KASP to determine the two materials with large differences in leaf wrinkle grades, Guichun 8 (wrinkle grade 0) and Y2017-1 (wrinkle grade 4), and the hybridization of these two materials Twenty F5 individuals with a wrinkle grade of 0 were produced.

[0031] 1. Identification of leaf wrinkle disease of soybean plants in the field

[0032] Reference Chen Wenjie et al. (2020) (Chen Wenjie, Chen Yuan, Wei Qingyuan, Guo Xiaohong, Tang Fuyue, Yang Meng, Ye Wandian, Liang Jiang. Occurrence characteristics and material disease grade identification of a soybean wrinkle disease [J]. Soybean Science, 2020, 39( 3)) the method for identifying the leaf wrinkle disease level of soybean per plant identifies the wrinkle leaf disease grade of each individual plant, wherein, the wrinkle leaf disease grade is divided into 5 disease grade standards:

[0033] Grade 0: normal, canopy bean leaves are not wrinkled during the observation...

Embodiment 3

[0049] Molecular markers were used to predict the wrinkle resistance of lines derived from Guichun 8 (wrinkle grade 0) and Y2017-1 (wrinkle grade 4).

[0050] Soybean to be tested: Guichun No. 8 is the female parent and Y2017-1 is the male parent. There are 253 individual plants in F5 and 205 individual plants in BC2F2.

[0051] Identification of leaf wrinkle of soybean plants in the field: refer to the identification method in Example 1.

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Abstract

The invention relates to the technical field of agricultural biology, and specifically discloses a KASP molecular marker for identification of soybean wrinkle disease resistance and its application. The KASP molecular marker primer sequences are shown in SEQ ID NO.1, SEQ ID NO.2 and SEQ ID NO.3, and the KASP molecular marker can realize efficient, accurate, low-cost identification and identification of soybean wrinkle disease resistance. Genotyping, the identification and detection effectiveness is 99.58%, and the accuracy is 100%.

Description

technical field [0001] The invention belongs to the technical field of agricultural biology, and particularly relates to a KASP molecular marker and application for identification of soybean wrinkle resistance. Background technique [0002] A phenomenon of soybean leaf shrinkage in production in Guangxi, Guangdong, Fujian, Guizhou and other places. The symptoms of this leaf wrinkle are: obvious "wrinkles" between the leaf veins, and there are light yellow spots on the leaves that are not obvious at the edges. The leaf margin is yellowish, the leaf is difficult to stretch, the top of the main vein is bent downward, the whole leaf is in the shape of "hook hand", and the symptoms at the base of the leaf are lighter than that at the top of the leaf. According to the investigation, this symptom has already existed in production, but it was mistakenly regarded as a virus disease in the past; wrinkled leaf disease is induced by an unknown factor in the environment, and can appear s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12Q1/6858C12N15/11
CPCC12Q1/6895C12Q1/6858C12Q2600/156C12Q2600/13C12Q2531/113C12Q2535/125C12Q2537/161C12Q2563/107
Inventor 陈文杰陈渊梁江郭小红汤复跃韦清源
Owner GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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