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Antibody diluent for immunohistochemical detection and its preparation method and application

An immunohistochemistry, antibody dilution technology, applied in chemical instruments and methods, immunoglobulins, biological testing, etc., can solve the problems of unfavorable safety experiments and safety management, poor storage stability of antibodies, and high toxicity of sodium azide, Achieve broad-spectrum bacteriostasis, maintain activity and stability, and enhance storage stability

Active Publication Date: 2021-12-17
图凌(杭州)生物医药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

PBS or PBST is used as the antibody diluent to reduce the impact of pH changes on the experimental results through its buffering effect, but this method has problems such as low detection intensity, poor antibody storage stability, and low detection sensitivity
There is a method of adding sodium azide preservative to enhance the storage stability of antibodies, but sodium azide is highly toxic and explosive, which is not conducive to safety experiments and safety management

Method used

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  • Antibody diluent for immunohistochemical detection and its preparation method and application
  • Antibody diluent for immunohistochemical detection and its preparation method and application
  • Antibody diluent for immunohistochemical detection and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1 Preparation of antibody dilution # 1- # 5

[0048] This example provides 5 antibody dilutions # 1- # 5 with Tris-HCl buffer as main components, including bovine serum albumin (BSA), double imidazoleyl urea antibacterial and Tween, specific formula Table 1 shows.

[0049] Table 1 antibody dilution # 1- # 5 formula

[0050]

[0051] The preparation method is as follows:

[0052] Accurately weighted or quantified BSA, double-imidazolidinyl urea, Tween in a clean sterilization vessel, add Tris-HCl buffer (pH 7.4), thoroughly stir mixing, then use 0.22 μm filter filtration and preservation spare.

Embodiment 2

[0053] Example 2 Preparation of antibody dilution # 6

[0054] This embodiment provides an antibody dilution # 6 with a PBS buffer as a main component, including bovine serum albumin (BSA), double imidazolidinylidethemia and Tween, and the specific formulation is shown in Table 2.

[0055] Table 2 antibody dilution # 6 formula

[0056] Component content 1 × PBS (ml) 999.5 Tween-20 (ml) 0.5 Double imidazoleyl (G) 2 BSA (g) 2.5

[0057] The preparation method is as follows:

[0058] Accurately weighted or quantitative BSA, double-imidazolidinyl urea, Tween in clean sterilization vessel, add 1 × PBS buffer (pH 7.4), thoroughly stir mixed, then use 0.22 μm filter filtration and preservation spare.

[0059] Among them, the formulation of 1 × PBS buffer is as follows:

[0060] 8.0 g sodium chloride, 0.2 g of potassium chloride, 2.9 g of sodium hydrogen phosphate diodium hydrogen phosphate diq, 0.2 g of phosphate dihydrogen, dissolved in 800 ml purified ...

Embodiment 3

[0061] Example 3 Preparation of antibody dilution # 7- # 9

[0062] This example provides three antibody dilutions # 7- # 9 with Tris-HCl buffer as main components, including bovine serum albumin (BSA), double-imidazolinyl ureacide and Tween, specific formula such as Table 3 shows.

[0063] Table 3 antibody dilution # 7- # 9 formula

[0064]

[0065] The preparation method is as follows:

[0066] Accurately weighted or quantified BSA, double-imidazolidinyl urea, Tween in a clean sterilization vessel, add Tris-HCl buffer (pH 7.4), thoroughly stir mixing, then use 0.22 μm filter filtration and preservation spare.

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Abstract

The invention discloses an antibody diluent for immunohistochemical detection and belongs to the technical field of antibody detection. The antibody diluent is a buffer containing bovine serum albumin with a mass fraction of 0.1% to 1%, diimidazolidinyl urea with a mass fraction of 0.1% to 0.5%, and Tween with a volume fraction of 0.01% to 0.1%. . The antibody diluent of the present invention can improve the intensity, sensitivity and stability of immunohistochemical detection, and the antibody diluent of the present invention can be used as a diluent of antibody products for the production of immunohistochemical antibody reagents, which can improve the Storage stability of histochemical antibody reagents.

Description

Technical field [0001] The present invention belongs to the field of antibody technology, in particular, it relates to antibody dilutions and preparation method and chemical detection immunohistochemistry. Background technique [0002] Immunohistochemistry (referred to immunohistochemistry) is using the principle of specific binding of antigen and antibody, substrate by chemical reaction color labeled antibody, to determine whether the target antigen and method for detecting the expression of tissue. It specific immune response, histochemical visibility combined by means of a microscope (including fluorescence microscopy, electron microscopy) imaging and amplification, in a cell, subcellular level detecting various antigenic material, its positioning, qualitative and quantitative. [0003] Antibody dilution formulation on the stability of the detected intensity of immunohistochemical antibody and plays a key role. Antibody dilution formulation using conventional immunohistochemis...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/53C07K16/00
CPCG01N33/53C07K16/00
Inventor 陶娜娜章月凯
Owner 图凌(杭州)生物医药有限公司
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