Quality control detection method of liquid phase chip analyzer and standard disc

Abstract

The invention discloses a quality control detection method of a liquid phase chip analyzer and a standard disc, and the method comprises the following steps: confirming that a sheath liquid bottle is filled with sheath liquid, and placing the standard disc filled with a standard reagent into the analyzer; adjusting and judging whether the standard disc is aligned with the analyzer or not; judging whether a sheath fluid system and a cleaning system of the analyzer are normal or not; measuring and analyzing the fluorescence value of the standard reagent, and judging whether the laser works normally or not; drawing a standard curve according to the fluorescence value of the standard reagent; positioning and calibrating a signal position of the analyzer according to the fluorescence value of the standard reagent. According to the method, the fluorescence value of a standard reagent is measured and analyzed, whether a standard disc is inserted in place or not and the operation condition of a laser are judged according to the fluorescence value, a standard curve is drawn, and signal position positioning calibration is performed, so that fluorescence value signal detection accuracy is effectively ensured; therefore, the analyzer can display the detection result of the sample to be detected in an effective range and at a proper position in actual use, and the accuracy of the experimental result is ensured.

Description

technical field [0001] The present application relates to the field of chip technology, in particular, to a quality control detection method and a standard plate of a liquid phase chip analyzer. Background technique [0002] The liquid phase chip analyzer is a high-throughput biochip detection device that integrates fluorescence-encoded microspheres, laser detection, flow cytometry, and data analysis. It can simultaneously detect up to 100 or even 500 different proteins, peptides, DNA or Target analytes such as RNA. [0003] Due to the good reproducibility of the data on the liquid chip analyzer, it is now widely used in immunology and various disease research, such as cardiovascular disease, tumor-specific marker Biomarker screening, drug therapy target development, drug therapy Effect evaluation, disease-related angiogenesis, metastasis, cell proliferation, apoptosis, inflammation, signal transduction, monitoring research of cytokine release syndrome of cell therapy, etc....

AI Technical Summary

Problems solved by technology

[0005] Existing quality control testing methods generally simply check whether each system in the analyzer can run smoothly. Under long-term use or improper storage of the analyzer, it is easy to cause pollution, which will lead to improper or even distortion of the fluorescence value display position of the test results. The operation of each system is difficult to guarantee the accuracy of the test results

[0006] For the above problems, there is no effective technical solution

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