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Plant lectin succ-Con A for treating corona virus infection and application

A technology of phytohemagglutinin and coronavirus, applied to phytohemagglutinin succ-ConA, phytohemagglutinin succ-ConA for treating coronavirus infection and application field

Active Publication Date: 2021-08-17
NAT INST FOR FOOD & DRUG CONTROL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] As the coronavirus continues to spread, its genome is also constantly mutating. Related studies have shown that the mutant strain D614G has been widely prevalent around the world. By December 2020, more than 98% of the sequences contained the D614G mutation (Korber B, Fischer W M, Gnanakaran S, et al.Tracking changes in SARS-CoV-2Spike:evidence that D614G increases infectivity of the COVID-19 virus[J].Cell,2020,182(4):812-827.e19.), in The British mutant strain and the South African mutant strain evolved on the basis of the N501Y mutant strain are currently mainly prevalent in the UK and South Africa. Although two mutant strains have been found in other countries, they have not yet formed outside the UK and South Africa. The main epidemic strains, in addition to these mutant strains, also include some mutant strains combined with other mutation sites, such as D614G+I472V, D614G+A435S, A222V+D614G, K417N+D614G, S477N+D614G, E484K+D614G, N501Y+ D614G

Method used

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  • Plant lectin succ-Con A for treating corona virus infection and application
  • Plant lectin succ-Con A for treating corona virus infection and application
  • Plant lectin succ-Con A for treating corona virus infection and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0086] Example 1 Phytohemagglutinin detection of SARS-CoV-2 S protein binding activity and antiviral activity

[0087] 1. Experimental materials

[0088] Lentil lectin, wheat germ agglutinin (WGA), sessile elderberry agglutinin (SSL), peanut lectin (Peanut lectin), Huaihuai agglutinin (MAL) were purchased from Wako (Japan) Datura agglutinin (DSL), succinyl-concanavalin A (succ-Con A), galactus agglutinin (GNL), phytohemagglutinin (PHA-E), leukolectin (PHA-L ), phytohemagglutinin-M (PHA-M) and phytohemagglutinin-P (PHA-P) from red kidney bean were purchased from Sigma Aldrich; SARS-COV-2 S trimer expressed in HEK293 cells was purchased from at ACRO Biosystems (China, Beijing); 293T cells were provided by the American Type Culture Collection (ATCC); Huh7 cells were provided by the Japanese Research Biological Resource Collection (JCRB); the SARS-CoV-2spike (Wuhan-Hu- 1, MN908947; hCoV-19 / South Africa / KRISP-K007869 / 2020, B.1.351, EPI_ISL_860630; hCoV-19 / England / QEUH-F56F0F / 2021...

Embodiment 2

[0102] Example 2 Detection of the Inhibitory Effect of Plant Hemagglutinin on the Infection of SARS-CoV-2 S Protein Mutants and SARS-CoV-2 Epidemic Mutants

[0103] In this example, the inhibitory effects of various plant lectins on the infection of SARS-CoV-2 S protein mutant strains and SARS-CoV-2 epidemic mutant strains, including the British epidemic mutant strain B.1.1.7, were detected. , South African popular mutant strain B.1.351, Brazilian popular mutant strain P.1, combined mutant strain D614G+I472V, combined mutant strain D614G+A435S, A222V, K417N, S477N, E484K, N501Y, combined mutant strain A222V+D614G, combined mutant strain Mutant strains such as strain K417N+D614G, combined mutant strain S477N+D614G, combined mutant strain E484K+D614G, combined mutant strain N501Y+D614G;

[0104] Lectins are mainly aimed at the glycosyl part of glycoproteins. In this example, the inventors studied whether the change of the glycation site of SARS-CoV-2 S protein would affect its n...

Embodiment 3

[0114] Example 3 Detection of Hemagglutination Activity and Cytotoxicity of Plant Hemagglutinin

[0115] Since many natural lectins can cause hemagglutination, in this example, the inventors studied the hemagglutination activity of different lectins, and studied the cytotoxicity of Huh7 and 293T cells.

[0116] 1. Detection of hemagglutination activity of phytohemagglutinin

[0117] Chicken blood samples were used to detect the hemagglutination activity of phytohemagglutinin, the rooster red blood cells were washed with PBS, and resuspended at a final concentration of 1% (v / v), and 50 μL of 2-fold serially diluted phytohemagglutinin solution was mixed with an equal volume of red blood cells The solution was mixed in a 96-well round-bottom plate, and a 2-fold serial dilution of influenza antigen (B / Maryland / 15 / 2016, NIBSC-UK-EN63QG, NIBSC code: 18 / 104, HA: 69 μg / mL) was performed and served as a positive control , PBS solution was used as a negative control, the plate was incu...

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PUM

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Abstract

The invention discloses a phytolectin succ-Con A for treating corona virus infection and an application thereof. The invention discovers that the phytolectin succ-Con A has a broad-spectrum antiviral effect on SARS-CoV-2 and SARS-CoV-2 mutant strains, SARS-CoV and MERS-CoV corona virus strains at the same time for the first time, and can be used as a broad-spectrum antiviral preparation or drug active ingredient. A new thought is provided for preventing and treating corona virus infection.

Description

technical field [0001] The present invention belongs to the technical field of medicine, in particular relates to a plant lectin succ-Con A, more specifically relates to a plant lectin succ-Con A for treating coronavirus infection and its application. Background technique [0002] The new coronavirus (2019-nCoV) was officially classified and named as severe acute respiratory syndrome coronavirus 2 (Severe acute respiratory syndrome coronavirus 2, SARS-CoV-2) by the International Virus Taxonomy Committee on February 12, 2020. The World Health Organization ( WHO) officially named the disease caused by SARS-CoV-2 as COVID-19 on the same day. SARS-CoV-2 belongs to the genus β-coronavirus and is a positive-strand RNA virus. Its nucleic acid sequence is 79.5% identical to SARS-CoV, and 96.2% identical to the Bat CoV RaTG13 strain of bat coronavirus. The betacoronaviruses detected in bats are more similar, and SARS-CoV-2 is the seventh member of the coronavirus family that infects...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/16A61P31/14
CPCA61K38/168A61P31/14
Inventor 王佑春王兰黄维金王文波李倩倩吴佳静段茂芹武刚于传飞郭璐韵
Owner NAT INST FOR FOOD & DRUG CONTROL
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