Preparation method of canavaline polymer hydrogel, prepared hydrogel and application thereof

A technology of concanavalin and polymers, which is applied in the field of polymer materials and can solve problems such as undisclosed hydrogel adhesion properties

Active Publication Date: 2021-09-10
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The patent application with publication number CN106883430A discloses a preparation method of vegetable protein composite hydrogel, which uses soybean protein isolate with good biocompatibility and good degradability as raw material, and

Method used

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  • Preparation method of canavaline polymer hydrogel, prepared hydrogel and application thereof
  • Preparation method of canavaline polymer hydrogel, prepared hydrogel and application thereof
  • Preparation method of canavaline polymer hydrogel, prepared hydrogel and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] Preparation of Mannose Monomer

[0070] (1) Preparation of mannose-bromide: 3g of D-mannose (dried in vacuum at 60°C for 2 hours before use of mannose) and 150mg of sulfuric acid-silicon dioxide were added to a round-bottomed flask with a magnet, Then 9 mL of propynyl alcohol was added, heated at 60°C for 6 h, and then purified by a silica gel column using a developing solvent of methanol and dichloromethane with a volume of 6:1. Mannitol-bromine was obtained as 2.6 g of a yellow oily substance. The preparation of mannose-bromine is a typical substitution reaction, and the reaction conversion rate is very high with the participation of a catalyst. Some monomers that have not been successfully substituted can be separated and purified by using a silica gel chromatography column to obtain pure mannose-bromine monomer . Wherein the sulfuric acid-silicon dioxide catalyst is a prior art, which is a mixture of sulfuric acid and silicon dioxide in granular form, wherein the ...

Embodiment 2

[0085] Preparation of photodiacetylamide

[0086] Dissolve 1 g of cystamine dihydrochloride in 20 mL of dry dichloromethane, then add 2.25 g of triethylamine, then slowly add 4 g of acryloyl chloride dropwise under stirring in an ice bath, after the dropwise addition is complete. React at room temperature for 2 h, then filter, and use ethyl acetate to separate through a silica gel column. The photodiacetamide was obtained as 0.5 g of white solid with a yield of 45%. image 3 It is the NMR spectrum of diacetamide.

[0087] The reaction equation is as follows:

[0088]

Embodiment 3

[0090] Preparation of HEPES buffer solution

[0091] The concentration of HEPES is 100mmol / L, the concentration of sodium chloride is 400mmol / L, the pH value of the solution is 7.2, the concentration of manganese chloride is 1mmol / L, and the concentration of calcium chloride is 1mmol / L.

[0092] When configuring HEPES buffer solution, mix HEPES and sodium chloride solution, adjust the pH value of the solution, and then add manganese chloride and calcium chloride, otherwise precipitation will occur.

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Abstract

The invention discloses a preparation method of canavaline polymer hydrogel, and relates to the technical field of polymer materials. The preparation method comprises the following steps that canavaline A is dissolved in a buffer solution, then mannose monomers, photodiacetamide, acrylamide and an initiator are added into the solution, and the canavaline polymer hydrogel is prepared after heating polymerization. The structural formula of the mannose monomer is shown in the specification, and the structural formula of the photodiacetamide is shown in the specification. The preparation method has the beneficial effects that the giant protein supramolecular monomer is constructed by combining the plant lectin tetramer canavaline and the double-bond modified mannose monomer, so that the polymer hydrogel has good adhesion performance and biocompatibility, and has good application prospects in the fields of drug release, tissue engineering and the like.

Description

technical field [0001] The invention relates to the technical field of polymer materials, in particular to a preparation method of concanavalin polymer hydrogel, the prepared hydrogel and applications thereof. Background technique [0002] With the development of industry, environmental pollution is becoming more and more serious. Traditional polluting polymer materials are gradually replaced by emerging environmentally friendly materials. Proteins are widely found in animal and plant life in nature. Natural protein-based soft networks have inherent self-healing properties. , large deformation, signal responsiveness, and surface adhesion to various substrates (soft and hard) are essential and important components in the process of realizing biological functions. It has been demonstrated that the participation of proteins in the network not only endows the network with better biological functions but also significantly increases the mechanical strength by enhancing the regula...

Claims

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Application Information

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IPC IPC(8): C08F289/00C08F220/58C08F222/38C08F220/56C07H1/00C07H15/04F21V23/02A61K47/32A61L27/16A61L27/52F21Y115/10
CPCC08F289/00C07H1/00C07H15/04F21V23/02A61K47/32A61L27/52A61L27/16F21Y2115/10C08F220/58C08F222/385C08F220/56C08L51/00
Inventor 杨光王婕韦曾明毕飞虎
Owner ANHUI AGRICULTURAL UNIVERSITY
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