Automatic sustainable large-scale 3D cell production system device
A production system and large-scale technology, applied in tissue cell/virus culture devices, animal cells, vertebrate cells, etc., can solve the problems of production cell quantity, quality and complex operation, achieve significant economic and social benefits, reduce Consumption cost, low cost effect
Pending Publication Date: 2021-09-24
SHANGHAI NAT ENG RES CENT FORNANOTECH
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Problems solved by technology
[0005] In order to overcome the problems of current production cell quantity, quality and complicated operation, the purpose of the present invention is to provide a system device for automatic sustainable large-scale 3D cell production, which is suitable for automatic large-scale 3D circulation culture of production cells under the G.M.P standard environment
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Embodiment
[0054] An automated large-scale 3D cell production system device, such as figure 1 As shown, it includes an intelligent gas distribution system 1, a production system 23, and a collection system 24, which are connected through a pipeline system 2;
[0055] The gas distribution system 1 is sealed and connected to the production system 23 through the pipeline system 2 and the pipeline connector 3, and the gas distribution system 1 provides the gas required for the production system 23 to produce cells;
[0056] Described production system 23 comprises first container 9, the motor 22 that is assembled on the first container 9, the stirrer 10 that is assembled in the first container 9 is driven by motor shaft 6 and rotates, produces cell 8, microcarrier 7 and culture medium 11 drop into the first container 9 from the feed inlet 5 of the first container 9, and control the temperature of the production system 23 by the temperature control system 4;
[0057] The collection system 24...
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Abstract
The invention relates to an automatic sustainable large-scale 3D cell production system device and application. The automatic sustainable large-scale 3D cell production system device comprises an intelligent gas distribution system, a production system and a collection system, and the intelligent gas distribution system can meet gas required by cell culture; the production system comprises production cells, a temperature control system, a microcarrier, a stirrer and a delivery pump, the temperature control system provides a good production environment for various cells, the microcarrier provides a good adhesion growth surface for the cells, the microsurface required by cell growth is greatly improved, and the 3D suspension culture of the cells is realized under the stirring of the stirrer; after the production cells are adhered to the surface of the microcarrier, the production cells serve as new cell seeds to be inoculated to the surface of the subsequently input microcarrier; and the collection system comprises a filter membrane, a second container, a material return port and a delivery pump, cyclic utilization of the culture medium and the microcarrier and continuous output of production cells are realized by using the delivery pump and the filter membrane, and 3D automatic sustainable large-scale cell production is achieved.
Description
technical field [0001] The invention relates to a system device for automatic sustainable large-scale 3D cell production. [0002] technical background [0003] Cells, especially various stem cells, have attracted widespread interest in clinical applications and scientific research due to their unique differentiation potential, repair ability, and targeted drug delivery performance. However, there are still various problems in the use of stem cells, the most notable of which is the large demand for stem cells that is difficult to solve. All kinds of cell therapy need to maintain a certain number of cells as much as possible in order to show its specific curative effect. In the process of traditional cell adherent culture, due to the limited two-dimensional (2D) surface area of the culture vessel, it needs to be continuously passaged to meet the demand for the number of cells. The use of liquid will affect the gene and protein expression of cells to a certain extent, and t...
Claims
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IPC IPC(8): C12M1/38C12M1/04C12M1/12C12M1/02C12M1/00C12M3/02C12N5/00C12N5/0775
CPCC12M25/14C12M27/02C12M29/26C12M41/12C12M29/18C12M33/14C12N5/0075C12N5/0062C12N5/0668C12N2513/00C12N2511/00C12N2531/00
Inventor 崔大祥彭家伟梁辉刘泽熙
Owner SHANGHAI NAT ENG RES CENT FORNANOTECH