Preparation method of stem cell apoptotic body for repairing endometrium and improving fertility

Abstract

The invention relates to the field of stem cell regeneration medicine. The invention aims to provide a preparation method of a stem cell apoptotic body for repairing endometrium and improving fertility. The obtained apoptotic body can regulate and control the wound surface microenvironment, effectively promote endometrium repair, remarkably improve the fertility rate and avoid potential safety hazards caused by traditional exogenous cell transplantation; and the method is simple in preparation process and high in operability. According to the technical scheme, the preparation method of the stem cell apoptotic body for repairing the endometrium and improving the fertility is not used for disease diagnosis and treatment; and the method comprises the following steps of: when the confluence degree of stem cells in a good growth state is greater than 75%, irradiating the stem cells with 500-700mJcm<-2> ultraviolet rays, continuously culturing the stem cells in a complete culture medium for 18-30 hours, taking cell culture supernatant, and carrying out centrifugal treatment to obtain a precipitate, i.e., the stem cell apoptotic body.

Description

technical field [0001] The invention relates to the field of stem cell regenerative medicine, in particular to a bioactive body for repairing endometrium and improving fertility. Background technique [0002] Intrauterine adhesions are the second leading cause of secondary infertility in women, mainly caused by trauma, infection, etc. Endometrial damage is accompanied by regeneration and repair disorders, endometrial fibrosis, and ultimately leads to intrauterine adhesions and endometrial atrophy. Endometrial fibrosis, as the main pathological feature of intrauterine adhesions, will not only form fibrous scars and lead to abnormal uterine cavity morphology leading to infertility or miscarriage, but also aggravate the vicious cycle of ischemia and hypoxia leading to aplastic impairment-fibrosis. The existing clinical treatment methods include: surgery, estrogen and postoperative barrier device placement. However, for patients with moderate to severe intrauterine adhesions, t...

AI Technical Summary

Problems solved by technology

However, there are limitations in the clinical application of cell therapy in endometrial repair, such as: loss of stemness during in vitro transportation and storage of stem cells, tumorigenicity of stem cells, immunogenicity of stem cells, difficulty in standardization of stem cell therapy, etc.

Therefore, living cells cannot be considered as off-the-shelf products for clinical application

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