Pleurotus ferulae ergosterol peroxide extraction method and application thereof

A technology of ergosterol peroxide and extraction method, which is applied in the field of medicine, and can solve the problems of low yield of ergosterol peroxide in ferulae ferulae

Pending Publication Date: 2021-10-01
XINJIANG QIANJIN RONGYAO INVESTMENT CO LTD
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in this method, the yield of ergosterol peroxide in ferulus ferulae is low, only 6.4mg, and the yield is 0.00256%.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Pleurotus ferulae ergosterol peroxide extraction method and application thereof
  • Pleurotus ferulae ergosterol peroxide extraction method and application thereof
  • Pleurotus ferulae ergosterol peroxide extraction method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Pulverizing the air-dried fruiting bodies of Ferula ferulae and passing through a 40-mesh sieve to obtain fruiting bodies of Ferulica ferulae powder;

[0067] Take 250g of Ferula ferulae fruiting body powder, extract it with 5L petroleum ether in a water bath at 60°C for 2h, and then carry out ultrasonic-assisted extraction of the extraction solution. The time of ultrasonic-assisted extraction is 20min, the temperature is 60°C, and the power is 300W. extract; centrifuge the extract at 5000rpm / min for 15min to obtain a filter residue and a filtrate; the filter residue is mixed with petroleum ether for leaching, ultrasonic-assisted extraction and centrifugation to obtain a filter residue and a filtrate; the filter residue and petroleum ether are mixed Extraction, ultrasound-assisted extraction and centrifugation are performed, and the extracts are combined.

[0068] The extract is concentrated by rotary evaporation at a temperature of 60° C.; after the concentration is co...

Embodiment 2

[0080] The difference between Example 2 and Example 1 is that the volume ratio of petroleum ether and ethyl acetate is replaced by 1:0, 8:2, 1:1, 7:3, 4:6, 2:8, 0:1 , to obtain 60.5mg Ferula ergosterol peroxide.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of medicine, and particularly relates to a pleurotus ferulae ergosterol peroxide extraction method and application thereof. The extraction method comprises the following steps: extracting pleurotus ferulae sporocarp powder with petroleum ether to obtain an extracting solution, and concentrating and drying the extracting solution to obtain an extract; dissolving the extract is dissolved to prepare a solution; carrying out silica gel column chromatography on the solution to obtain a crude product; subjecting the crude product to sephadex column chromatography to prepare the pleurotus ferulae ergosterol peroxide. The yield of the pleurotus ferulae ergosterol peroxide extracted by the extraction method is greatly improved, so that a foundation is laid for the application of the pleurotus ferulae ergosterol peroxide in antitumor drugs.

Description

technical field [0001] The invention belongs to the technical field of medicines, and in particular relates to a method for extracting ergosterol peroxide from ferulae ferulae and its application. Background technique [0002] Ergosterol peroxide (Ergosterol peroxide, EP, C 28 h 44 o 3 ), is a C28-sterol with a unique chemical structure, forming an -O-O- between the steroid core C-5 and C-8, belonging to the sterol 5α, 8α epoxy derivatives, and is a variety of food, medicine The active ingredients of plants and medicinal fungi have a wide range of pharmacological activities, including anti-microbial, anti-malarial, anti-inflammatory, anti-tumor, immune regulation and anti-oxidation. EP can inhibit the proliferation of tumor cells in vitro and in vivo by acting on signaling pathways such as β-catenin, JAK2 / STAT3, NF-κB and MAPKs. Studies have reported that Pleurotusferulae ergosterol peroxide (PFEP) can induce tumor cell apoptosis through endoplasmic reticulum stress and ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07J71/00A61P35/00
CPCC07J71/0005A61P35/00
Inventor李金耀马荣斌玛衣拉·牙生夏丽洁
OwnerXINJIANG QIANJIN RONGYAO INVESTMENT CO LTD