Determination method for ginsenoside Re content in Shenbeiguchang capsule

A technology of Shenbei Guchang and ginsenosides, which is applied in the field of determination of ginsenoside Re content in Shenbei Guchang capsules, can solve problems such as difficult to accurately detect the content of ginsenoside Re, so as to improve column efficiency, increase efficiency, reduce The effect of intermediate loss

Inactive Publication Date: 2019-08-30
GUIZHOU LIANSHENG PHARMA
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a method for determining the content of ginsenoside Re in Shenbei Guchang Capsules, which eliminates the interference of other traditional Chinese medicine ingredients in Shenbei Guchang Capsules on the detection of ginsenoside Re, thereby solving the problem of the difficulty in accurately detecting ginsenoside Re. Problems with the Content of Ginsenoside Re in Beiguchang Capsules

Method used

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  • Determination method for ginsenoside Re content in Shenbeiguchang capsule
  • Determination method for ginsenoside Re content in Shenbeiguchang capsule
  • Determination method for ginsenoside Re content in Shenbeiguchang capsule

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Embodiment 1

[0037] Embodiment 1: step 1): using methanol solution as a solvent to prepare a reference substance solution;

[0038] Step 2): Prepare the test solution after heating the content of Shenbei Guchang Capsules to reflux;

[0039] Step 3): Determination of the content of ginsenoside Re in the reference substance solution and the test sample solution by high performance liquid chromatography;

[0040] The chromatographic detection conditions are: using octadecylsilane bonded silica gel as filler; using acetonitrile as mobile phase A and water as mobile phase B for gradient elution; flow rate 1mL / min, detection wavelength 203nm; injection volume 20μl;

[0041] Further, the specific operation for preparing the reference substance solution in step 1) is: accurately weigh the ginsenoside Re reference substance, add methanol to dissolve, and obtain a reference substance solution with a concentration of 0.2 mg / mL;

[0042] Further, the specific operation of step 2):

[0043] Step 2) s...

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Abstract

The invention discloses a determination method for ginsenoside Re content in a Shenbeiguchang capsule. The determination method comprises the following steps: 1) preparing reference solution by takingmethanol solution as a solvent; 2) performing heating and refluxing on Shenbeiguchang capsule content, and then preparing test solution; 3) determining ginsenoside Re contents in the reference solution and the test solution by adopting a high performance liquid chromatography method under the chromatographic detection conditions that octadecyl silane bonded silica gel is taken as a filling agent,gradient elution is performed by taking acetonitrile as a mobile phase A and water as a mobile phase B, flow velocity is 1mL / min, detection wavelength is 203nm and sample size is 20[mu]l. The technical scheme adopted by the invention solves the problems that interference on ginsenoside Re detection can be easily caused as the Shenbeiguchang capsule also contains all kinds of other traditional Chinese medicine ingredients and then the ginsenoside Re content in the Shenbeiguchang capsule is difficult to detect.

Description

technical field [0001] The invention relates to the technical field of drug component detection, in particular to a method for determining the content of ginsenoside Re in Shenbei Guchang Capsules. Background technique [0002] Shenbei Guchang Capsule is a Chinese patent medicine for disharmony between liver and spleen, diarrhea and abdominal pain, and chronic non-specific ulcerative colitis. Woody, herb, Poria cocos, deer horn cream, ginseng, etc.; wherein the active ingredient of ginseng is ginsenoside Re, ginsenoside Re can inhibit the central nervous system, promote the synthesis of DNA and RNA, increase the effect of plasma corticosterone, dilate blood vessels, and It has anti-fatigue effect; although there are some methods for detecting ginsenoside Re by HPLC at present, because Shenbei Guchang Capsule is a pure Chinese medicine preparation, the prescription contains 9 Chinese medicinal materials and 2 kinds of auxiliary materials, and other Chinese medicine ingredie...

Claims

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Application Information

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Patent Type & AuthorityApplications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor聂安军周英徐会
OwnerGUIZHOU LIANSHENG PHARMA