Adult t-cell leukemia-lymphoma detection method
A detection method and technology for adults, applied in the field of detection of adult T-cell leukemia/lymphoma, which can solve problems such as poor long-term prognosis
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[0132] The present invention is further described in detail below by way of examples. However, the present invention is not limited to the following examples.
[0133] In order to extract the abnormal DNA methylation region that is very important for the pathological formation of ATL, lymphocyte separation medium (lymphocyte separation medium, MP Biomedical) was used according to the instruction manual, from healthy people (3 people), HTLV-1 infected people (3 people) Peripheral blood mononuclear cells were isolated from the peripheral blood of patients with smoldering ATL (3 patients), patients with chronic ATL (4 patients), and patients with acute ATL (3 patients), and the cell surface was further separated by a cell sorter (BD Biosciences). Expression patterns of the proteins CADM1 and CD7 segregating the fraction of normal T cells (part P) and the fraction of T cells infected with HTLV-1 (parts D, N). This cell isolation method is described in detail in the paper publishe...
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