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Preparation and application of a mesenchymal stem cell preparation

A stem cell preparation, mesenchymal stem cell technology, applied in the field of preparation of mesenchymal stem cell preparations, can solve the problems of exacerbation of tumor growth, long culture time, failure to alleviate the course of cancer, etc.

Pending Publication Date: 2021-10-19
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, many shortcomings have been gradually discovered in the application process of autologous mesenchymal stem cells
(1) There are great individual differences in the expansion ability; (2) The culture time is long and cannot meet the needs of the disease in time; (3) It has been reported that BM-MSCs derived from cancer patients can also protect tumor cells from chemotherapy drugs. Killing effect
Therefore, autologous transplantation of BM-MSCs cannot achieve the effect of alleviating the course of cancer, and may even exacerbate tumor growth.
These problems seriously restrict the clinical application of autologous mesenchymal stem cells

Method used

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  • Preparation and application of a mesenchymal stem cell preparation
  • Preparation and application of a mesenchymal stem cell preparation
  • Preparation and application of a mesenchymal stem cell preparation

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Embodiment Construction

[0052] The principles and features of the present invention are described below in conjunction with examples, which are only used to explain the present invention and are not used to limit the scope of the present invention.

[0053] Isolation and subculture of umbilical cord mesenchymal stem cells

[0054] Prepare tissue digestion enzymes (type II collagenase 250U / ml, dispase 100U / ml, hyaluronidase 10U / ml), dissolve in α-MEM medium at 37°C, filter and sterilize with a 0.22μm filter for later use;

[0055] Take the umbilical cord tissue of the newborn baby, put the umbilical cord into a petri dish, wash it with PBS containing 0.1% penicillin-streptomycin double antibody, put it in α-MEM medium, peel off the three blood vessels, and cut the umbilical cord into 1- 2mm 3 organization block;

[0056] Mix the shredded tissue pieces and the prepared tissue digestion enzyme solution in a 1:1 volume ratio in a 50ml centrifuge tube, digest at 37°C, 200rpm for 3 hours, and wait until ...

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Abstract

The invention belongs to the technical field of medical biology, and particularly relates to a preparation method and application of a mesenchymal stem cell preparation. According to the invention, human-derived mesenchymal stem cells (MSCs) are treated by an activating agent composed of a plurality of specific bioactive molecules and compounds to obtain activated mesenchymal stem cells (Active MSCs). The Active MSCs specifically secrete a large amount of various bioactive substances such as interleukin 6 (IL6), indoleamine 2, 3-dioxygenase (IDO), bone morphogenetic protein 6 (BMP6), neutrophil elastinase, cathepsin G, superoxide dismutase, tissue metalloproteinase inhibitory factor 3 (TIMP3), protein lysine 6 oxidase (LOX), IGFL3, TNFAIP6, CYR61, TGF beta and the like. The preparation can treat autoimmune diseases, improve the anti-tumor ability of the body, prevent aging, enhance the immunity of the body and promote the regeneration ability of the body tissue. The active MSCs obtained by inducing and activating the mesenchymal stem cells by adopting the mesenchymal stem cell activating agent have no drug residues and can highly express bioactive substances with immunoregulation and tissue regeneration capacities. Compared with chemotherapy, the active MSCs transplantation has lower toxic and side effects, has low immunogenicity, and has great significance in treatment of tumors and immune diseases and prevention of aging.

Description

technical field [0001] The invention belongs to the field of stem cell therapy, and in particular relates to a preparation method and application of a mesenchymal stem cell preparation. Background technique [0002] Mesenchymal stem cells (MSCs) are multipotent adult stem cells that are widely distributed in mesenchymal tissues, such as fat and bone marrow. Mesenchymal stem cells not only have the same strong self-renewal and multi-directional differentiation potential as other stem cells, but also have the following three characteristics: (1) Wide range of sources, easy to separate, cultivate and purify, and still have the characteristics of stem cells after multiple passages. (2) MSCs interact with immune cells in the innate and adaptive immune system through direct contact or paracrine to exert their immune regulation function; (3) MSCs have low immunogenicity, so allogeneic transplantation The type requirements are not strict, and the rejection reaction is mild; (4) MSC...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775A61K35/28A61P35/02
CPCC12N5/0662C12N5/0665A61K35/28A61P35/02
Inventor 沈萍萍孙璐琛杨南飞郑薇左诗曼
Owner NANJING UNIV
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