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Bionic micro-fluidic chip for simulating cervical microenvironment and preparation method of bionic micro-fluidic chip

A microfluidic chip and microenvironment technology, applied in biochemical equipment and methods, chemical instruments and methods, and microbial determination/inspection, etc., can solve the problems of sperm damage, large sample and time consumption, and no unified standard, etc. Achieve the effect of reduced interference, rapid sperm screening, and small sample size

Active Publication Date: 2021-10-22
FUDAN UNIV
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  • Claims
  • Application Information

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Problems solved by technology

At present, the methods of sperm screening are mainly upstream method and ultracentrifugation method, both of which require a large amount of samples and time, and there is a lot of human interference, and there is no uniform standard, especially centrifugation will cause damage to the DNA of sperm

Method used

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  • Bionic micro-fluidic chip for simulating cervical microenvironment and preparation method of bionic micro-fluidic chip
  • Bionic micro-fluidic chip for simulating cervical microenvironment and preparation method of bionic micro-fluidic chip
  • Bionic micro-fluidic chip for simulating cervical microenvironment and preparation method of bionic micro-fluidic chip

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Embodiment Construction

[0045] The present invention will be described in further detail below in conjunction with the examples and accompanying drawings, but the embodiments of the present invention are not limited thereto.

[0046] A bionic microfluidic chip for simulating the microenvironment of the cervix in the female reproductive system, comprising a sample injection pool 1, a screening channel 3, and a collection pool 4. The sampling pool is provided with a microcolumn array 2, and the screening channel is filled with hyaluronic acid solution. The viscosity of the hyaluronic acid solution is similar to that of human cervical mucus ( image 3 ).

[0047]In this biomimetic microfluidic chip, the sampling pool and collection pool at both ends are circular, with a diameter of 0.5-2mm and a depth of 50 µm; the spacing of the microcolumns is 50 µm, and the diameter is 50 µm; they are evenly distributed in the form of an array In the sample pool, the function of the microcolumn is to separate the i...

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Abstract

The invention belongs to the technical field of micro-fluidic chips, and particularly relates to a bionic micro-fluidic chip for simulating a cervical microenvironment and a preparation method of the bionic micro-fluidic chip. The bionic micro-fluidic chip is provided with a sample introduction pool, a plurality of microcolumns, a screening channel and a collection pool. The plurality of microcolumns are uniformly distributed in the sample introduction pool and are used for filtering impurities in original semen to be detected. The sample introduction pool communicates with the collection pool through a plurality of screening channels which are parallel to one another. The bionic micro-fluidic chip is used for screening a large number of qualified high-quality sperms which can be directly applied to an assisted reproduction technology. The screening channel is filled with a trace hyaluronic acid solution with the function similar to that of cervical mucus and is used for simulating a cervical microenvironment. The chip disclosed by the invention can be used for screening sperms, identifying gene defect sperms and simply and quickly screening out infertility people caused by gene defects. The bionic micro-fluidic chip is simple and convenient to operate, low in cost and high in practicability.

Description

technical field [0001] The invention belongs to the technical field of microfluidic chips, and in particular relates to a bionic microfluidic chip for simulating the cervical microenvironment in the female reproductive system and a preparation method thereof. Background technique [0002] According to the assessment of the World Health Organization (WHO), about 1 in every 7 couples has reproductive disorders. Reproductive disorders not only put infertile couples under great psychological pressure, but can even lead to family and social problems such as divorce. Assisted reproductive technology is a direct means to realize the desire of infertile couples to conceive and have children, and to solve related problems caused by infertility. According to clinical statistics, about 20% of couples with infertility patients need to use assisted reproductive technology to have children. [0003] Impaired reproductive function in infertile couples is associated with altered sperm pen...

Claims

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Application Information

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IPC IPC(8): C12M1/34C12M1/00C12Q1/02B01L3/00
CPCC12M41/46C12M23/16G01N33/5005B01L3/502707B01L3/502761
Inventor 刘妍君经光银施惠娟余赛西罗昊刘亚楠吴怡
Owner FUDAN UNIV
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