Molecular marker for identifying duck slaughter traits based on myostatin gene MSTN and identification method and application thereof

A technology of molecular markers and identification methods, applied in biochemical equipment and methods, recombinant DNA technology, determination/inspection of microorganisms, etc., can solve problems such as lack of research on growth and development laws, and achieve high accuracy, time saving and cost saving Effect

Inactive Publication Date: 2021-10-22
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The Q strain of white-feathered meat duck is a newly selected strain, which has been continuously selected for 6 generations, but there is a lack of research on its growth and development.

Method used

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  • Molecular marker for identifying duck slaughter traits based on myostatin gene MSTN and identification method and application thereof
  • Molecular marker for identifying duck slaughter traits based on myostatin gene MSTN and identification method and application thereof
  • Molecular marker for identifying duck slaughter traits based on myostatin gene MSTN and identification method and application thereof

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Experimental program
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Embodiment Construction

[0032] The application will be described in further detail below in conjunction with the accompanying drawings. It is necessary to point out that the following specific embodiments are only used to further illustrate the application, and cannot be interpreted as limiting the protection scope of the application. The above application content makes some non-essential improvements and adjustments to this application.

[0033] 1. Materials

[0034] The methods used in this example are conventional methods known to those skilled in the art unless otherwise specified, and the reagents and other materials used are all commercially available products unless otherwise specified.

[0035] 2. Method

[0036] 2.1 Obtain polymorphic sites of duck MSTN gene

[0037] 2.1.1 Genomic DNA extraction and detection

[0038] 400 Q-series white-feathered meat ducks were selected as test materials, blood was collected from duck wing veins at 6 weeks of age, and blood DNA was extracted using the bl...

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Abstract

The invention discloses a molecular marker for identifying duck slaughter traits based on a myostatin gene MSTN as well as an identification method and application of the molecular marker. The molecular marker is T or C, the molecular marker is located at the 458th site of a myostatin gene MSTN, and the myostatin gene MSTN has a nucleotide sequence as shown in SEQ ID NO. 1. The duck slaughter traits are selected according to genotypes by utilizing the polymorphism of the 458th basic group on the CDS sequence exon1 of the MSTN gene. The method for selecting the slaughter traits of the ducks has the advantages of early selection, time saving, simple operation, cost saving, high accuracy and the like.

Description

technical field [0001] The invention relates to the technical field of molecular markers, in particular to a molecular marker for identifying duck slaughter traits based on myostatin gene MSTN, an identification method and application thereof. Background technique [0002] Duck meat is an important source of high-quality protein for Chinese consumers. It has a unique flavor and is rich in unsaturated fatty acids that are beneficial to human health. It accounts for about 25% of poultry consumption and is very popular among consumers. Meat ducks mainly include white-feather ducks, hemp-feather ducks, Muscovy ducks, etc. Among them, the output of white-feather ducks accounts for 80% of the total output of meat ducks. At present, the main breeds of white-feathered meat ducks are Cherry Valley meat ducks and Z-type Peking ducks. Q strain white-feathered meat duck is a newly selected strain, which has been continuously selected for 6 generations, but there is little research on i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/156C12Q2600/124
Inventor 金四华耿照玉江洪峰肖程税斐贾羽晴夏晶晶郑书丽何培莉王野
Owner ANHUI AGRICULTURAL UNIVERSITY
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