Application of protein markers in combination in diagnosis of type 2 diabetes mellitus and type 2 diabetic nephropathy

A technology of type 2 diabetes and protein markers, which is applied in the diagnosis of type 2 diabetic nephropathy. Protein markers are combined in the field of type 2 diabetes to achieve high diagnostic efficiency.

Pending Publication Date: 2021-10-26
JIANGSU PROVINCIAL HOSPITAL OF TCM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, currently used diagnostic methods have considerable limitations both in the early stages of DKD and in the diagnosis of its proteinuric progression

Method used

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  • Application of protein markers in combination in diagnosis of type 2 diabetes mellitus and type 2 diabetic nephropathy
  • Application of protein markers in combination in diagnosis of type 2 diabetes mellitus and type 2 diabetic nephropathy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1: α2-Macroglobulin, Cathepsin D, and CD324 protein combined diagnosis of healthy subjects, type 2 diabetes patients, patients with early type 2 diabetic nephropathy and patients with advanced type 2 diabetic nephropathy.

[0016] 1. Experimental samples and reagents

[0017] Samples: 30 healthy subjects (HC), 30 patients with simple type 2 diabetes mellitus (2-DM), 30 patients with early type 2 diabetic nephropathy (DKD-E) and 30 patients with type 2 diabetic nephropathy were collected from Jiangsu Provincial Hospital of Traditional Chinese Medicine Patients with advanced stage (DKD-A) were tested, and there was no significant difference in age, gender and body mass index matching of patients in each group;

[0018] Healthy subjects are healthy people with normal physical examination indicators, patients with type 2 diabetes, patients with early type 2 diabetic nephropathy and patients with advanced type 2 diabetic nephropathy. The inclusion criteria are as fol...

Embodiment 2

[0052] Embodiment 2: Diagnostic kit

[0053] A kit for diagnosing type 2 diabetes and type 2 diabetic nephropathy, the kit contains detection reagents for detecting CathepsinD, α2-Macroglobulin and CD324 proteins; wherein, the protein markers are Cathepsin D, α2-Macroglobulin and CD324; the Diagnosis of type 2 diabetes and type 2 diabetic nephropathy includes diagnosis and distinction between healthy people and type 2 diabetes patients, diagnosis and distinction between healthy people and type 2 diabetic nephropathy patients, diagnosis and distinction between type 2 diabetes patients and type 2 diabetic nephropathy patients, and diagnosis and differentiation of type 2 diabetes Patients with early nephropathy and type 2 diabetic patients with advanced nephropathy.

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Abstract

The invention discloses an application of protein markers in combination in diagnosis of type 2 diabetes mellitus and type 2 diabetic nephropathy. The protein markers are Cathepsin D, alpha2-Macroglobulin and CD324. The protein markers can be jointly used for diagnosing and distinguishing healthy people from type 2 diabetic patients, diagnosing and distinguishing healthy people from type 2 diabetic nephropathy patients, diagnosing and distinguishing type 2 diabetic patients from type 2 diabetic nephropathy late patients, and diagnosing and distinguishing type 2 diabetic nephropathy early patients from type 2 diabetic nephropathy late patients, and is high in diagnosis efficiency. The protein markers have a prospect of being developed into a kit for diagnosing type 2 diabetes and type 2 diabetic nephropathy.

Description

technical field [0001] The invention belongs to the field of disease detection and diagnosis, and relates to the discovery and application of disease diagnosis markers, in particular to the use of protein markers combined for the diagnosis of type 2 diabetes and type 2 diabetic nephropathy. Background technique [0002] Diabetic kidney disease (DKD) refers to chronic kidney disease (CKD) caused by diabetes. DKD is a common chronic complication of diabetes and is an end-stage renal disease (ESRD) important etiology. In CKD stages 1-2, there are few symptoms and are usually not detected until late stages. As CKD progresses, the risk of cardiovascular morbidity and mortality increases. [0003] DKD is a clinical diagnosis based on elevated urinary albumin and / or estimated decreased glomerular filtration rate while excluding other CKD. Microalbuminuria (urinary microalbumin / urinary creatinine 30-300 mg / g) is recognized as an early clinical marker of type 1 and type 2 diabetic...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/573
CPCG01N33/6893G01N33/6872G01N33/573G01N2800/347G01N2800/042G01N2333/4713G01N2333/70596G01N2333/96472
Inventor 刘史佳周栋安晓飞徐婷婷魏琼张露朱琳王颖卓
Owner JIANGSU PROVINCIAL HOSPITAL OF TCM
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