Auxiliary diagnostic reagent for bronchial asthma

A technology for bronchial asthma and auxiliary diagnosis, applied in the field of biomedicine, can solve the problems of difficult detection of asthmatic patients, and achieve the effect of economical detection method, easy development, and auxiliary early diagnosis

Inactive Publication Date: 2022-05-10
JIANGSU PROVINCE HOSPITAL THE FIRST AFFILIATED HOSPITAL WITH NANJING MEDICAL UNIV
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Problems solved by technology

[0003] Traditional asthma diagnosis mainly relies on clinical manifestations, pulmonary function tests, and peak flow rate detection, but the above-mentioned technologies are not easy to detect asthma patients with no obvious symptoms. With the development of biotechnology, biomarkers provide a new method for the diagnosis of asthma. way

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  • Auxiliary diagnostic reagent for bronchial asthma
  • Auxiliary diagnostic reagent for bronchial asthma

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Effect test

Embodiment 1

[0016] 1. Collection and cryopreservation of peripheral blood samples

[0017] The peripheral blood samples of 34 patients with bronchial asthma came from Jiangsu Provincial People's Hospital, and the samples of 30 healthy people came from college students of Nanjing Medical University. Collect peripheral blood and anticoagulant tubes from normal people and asthma patients, centrifuge at 1000rpm / min for 5 minutes, collect the upper layer of plasma, and freeze it at -80°C

[0018] 2. Detection of the expression of proline in human plasma

[0019] This kit uses a proline detection kit to determine the level of human proline in plasma samples. Proline is extracted with sulfosalicylic acid (SA), and proline is free in the solution of sulfosalicylic acid. After heat treatment with ninhydrin, the proline in the solution reacts with the acidic ninhydrin solution , the color turns red, the substance has a greater solubility in toluene, after extraction with toluene, the red pigment ...

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Abstract

The invention discloses an auxiliary diagnostic reagent for bronchial asthma. The reagent for detecting proline is applied to preparation of an auxiliary diagnostic reagent for bronchial asthma. The invention also discloses application of the reagent for extracting and detecting proline in preparation of the auxiliary diagnostic reagent for bronchial asthma. The auxiliary diagnostic reagent for bronchial asthma comprises a reagent for extracting proline from serum and a reagent for quantitatively detecting proline. It is found for the first time that the plasma proline level of an asthma patient is higher than that of a normal person, and statistical significance is achieved. When the cutoff value of proline is 38.7 ug / mL, the area under an ROC curve for diagnosing asthma is 0.951 (P is less than 0.0001), which indicates that the diagnosis efficiency is relatively high. Plasma proline is used as a biomarker, a specimen is easy to take, alveolar lavage is not needed, the method belongs to noninvasive examination, early diagnosis of asthma can be assisted, a proline detection method is economical and easy to carry out, and a potential target spot is provided for diagnosis and treatment of bronchial asthma.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to an auxiliary diagnostic reagent for bronchial asthma. Background technique [0002] Asthma is a heterogeneous disease characterized by chronic airway inflammation, airway remodeling, and airway hyperresponsiveness. There are about 300 million asthma patients in the world, and the number of asthma patients in my country exceeds 40 million. In recent years, with the progress of industrialization, the prevalence of asthma in my country is still increasing year by year, which has brought heavy family and social burdens. Due to the heterogeneity of asthma, asthma has multiple phenotypes and endotypes. The Global Initiative for Asthma (GINA) 2020 divides asthma into the following phenotypes: allergic asthma, nonallergic asthma, delayed-onset asthma, asthma with fixed airflow limitation, and asthma with obesity. Asthma populations with different phenotypes respond differently to drugs, especi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/64G01N33/50
CPCG01N33/6812G01N33/64G01N33/50G01N2800/122
Inventor 吉宁飞徐婷婷袁琪张希杰吴晶晶蒋静娴王正霞陈中琦张明顺黄茂
Owner JIANGSU PROVINCE HOSPITAL THE FIRST AFFILIATED HOSPITAL WITH NANJING MEDICAL UNIV
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