Adipose tissue-derived stem cell osteogenic induction composition and osteogenic induction method

A technology of stem cells and compositions, applied in the field of osteogenic induction compositions, can solve the problems of limiting the wide application of adipose-derived mesenchymal stem cells, low osteogenic induction rate, adverse reactions of the body, etc., so as to promote the proliferation of osteoblasts, promote Osteogenic differentiation, guaranteeing the effect of application

Active Publication Date: 2021-11-16
赛尔医学科技(山东)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, at present, the osteogenic induction of adipose-derived mesenchymal stem cells also has some problems common to ordinary stem cells, such as low osteo...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] An osteogenic induction composition of adipose-derived mesenchymal stem cells, consisting of the following raw materials: DMEM / F12 medium, disodium β-glycerophosphate, lithium iron phosphate, nano-bismuth selenide, paulownin, fibroblast growth factor, recombinant Insulin; in terms of final concentration, the concentration of each component in the medium is: 3 ng / mL of β-glycerophosphate disodium, 1.2 ng / mL of lithium iron phosphate, 3 ng / mL of nano bismuth selenide with an average particle size of 100 nm, Paulownin 4 ng / mL, fibroblast growth factor 8 μg / mL, recombinant insulin 3 μg / mL.

[0027] A method for osteogenic induction of adipose-derived mesenchymal stem cells, comprising the following process: taking the cultured P3 adipose-derived mesenchymal stem cells and adding an osteogenic induction composition with a cell density of 1×10 4 cells / mL, induce differentiation in a 6-well plate, and induce differentiation at 37°C, 5% CO 2 It was carried out in an incubator,...

Embodiment 2

[0029] An osteogenic induction composition of adipose-derived mesenchymal stem cells, consisting of the following raw materials: DMEM / F12 medium, disodium β-glycerophosphate, lithium iron phosphate, nano-bismuth selenide, paulownin, fibroblast growth factor, recombinant Insulin; in terms of final concentration, the concentration of each component in the medium is: β-glycerophosphate disodium 1ng / mL, lithium iron phosphate 0.5ng / mL, and nano-bismuth selenide with an average particle size of 150nm 2.5 ng / mL , Paulownin 3 ng / mL, fibroblast growth factor 5 μg / mL, recombinant insulin 1 μg / mL.

[0030] A method for osteogenic induction of adipose-derived mesenchymal stem cells, comprising the following process: taking cultured P3 adipose-derived mesenchymal stem cells and adding an osteogenic induction composition, the cell density is 3×10 4 cells / mL, induce differentiation in a 6-well plate, and induce differentiation at 37°C, 5% CO 2 It was carried out in an incubator, and the me...

Embodiment 3

[0032] An osteogenic induction composition of adipose-derived mesenchymal stem cells, consisting of the following raw materials: DMEM / F12 medium, disodium β-glycerophosphate, lithium iron phosphate, nano-bismuth selenide, paulownin, fibroblast growth factor, recombinant Insulin: In terms of final concentration, the concentration of each component in the medium is: 5 ng / mL of β-glycerophosphate disodium, 1.8 ng / mL of lithium iron phosphate, and 4.5 ng / mL of nano-bismuth selenide with an average particle size of 200 nm , Paulownin 5 ng / mL, fibroblast growth factor 10 μg / mL, recombinant insulin 5 μg / mL.

[0033] A method for osteogenic induction of adipose-derived mesenchymal stem cells, comprising the following process: taking cultured P3 adipose-derived mesenchymal stem cells and adding an osteogenic induction composition, the cell density is 5×10 4 cells / mL, induce differentiation in a 6-well plate, and induce differentiation at 37°C, 5% CO 2 It was carried out in an incubato...

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Abstract

The invention discloses an adipose tissue-derived stem cell osteogenic induction composition which is prepared from the following raw materials: a basic culture medium, beta-glycerophosphate disodium, lithium iron phosphate, nano bismuth selenide, paulownia element, fibroblast growth factors and recombinant insulin. The composition is added with the lithium iron phosphate, the nano bismuth selenide, the paulownin and other components, so that the osteogenesis proliferation and differentiation efficiency of cells can be effectively improved. The added lithium iron phosphate and nano bismuth selenide improve the osteogenic differentiation signal activity of the adipose-derived stem cells, and can effectively promote the osteogenic differentiation of the cells. The paulownin, the fibroblast growth factor and the recombinant insulin together promote osteoblast proliferation and shorten the cell differentiation time. The invention also provides an adipose tissue-derived stem cell osteogenic induction method, by adopting the composition, the adipose-derived mesenchymal stem cells can be efficiently and stably subjected to osteogenic induction differentiation, and the application of the adipose-derived mesenchymal stem cells in bone tissue engineering is guaranteed.

Description

technical field [0001] The present invention relates to an osteogenic induction composition, in particular to an osteogenic induction composition of adipose mesenchymal stem cells and an osteogenic induction method. Background technique [0002] Stem cells are the original source of various tissues and organs in the body, with the ability to proliferate and differentiate. With the rapid development of medical technology, stem cells have played an important role in cell transplantation, tissue engineering and regenerative medicine. In particular, bone tissue engineering is the fastest-growing subject in the field of tissue engineering, and stem cells are the main seed cells of tissue engineering. [0003] Adipose-derived mesenchymal stem cells are a type of stem cells with obvious advantages. Adipose tissue is the most accessible source of mesenchymal stem cells. Adipose tissue contains a large number of undifferentiated stem cells with abundant reserves. Compared with bon...

Claims

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Application Information

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IPC IPC(8): C12N5/077C12N5/0775
CPCC12N5/0654C12N2506/1384C12N2500/42C12N2500/24C12N2500/20C12N2501/999C12N2501/33C12N2501/115
Inventor 辛江江张坤
Owner 赛尔医学科技(山东)有限公司
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