Selenized cherokee rose fruit polysaccharide capable of enhancing immunoregulation and antioxidant activity as well as preparation method and application thereof
A technology for antioxidant activity and enhancing immunity, which is applied in the field of bioengineering to achieve the effects of favorable absorption, low production cost and low loss rate of polysaccharides
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Embodiment 1
[0039] Preparation and structure characterization of selenized polysaccharides from Rosa chinensis:
[0040] (1) Preparation of crude polysaccharides of Fructus Rosa Fructus: wash and dry the Fructus Rosa Fructus pulp, crush it and pass through a 100-mesh sieve to obtain the crushed Fructus Rosa Fructus pulp, and put it in a dry place for later use; condense the above crushed substance with 95% ethanol Reflux, the reflux temperature is 70°C, the reflux time is 2h, impurities and small molecular substances are removed, the supernatant is discarded by suction filtration, and the filter residue is naturally dried for later use; the filter residue is added with water at a ratio of 1:30 (g / mL), and the Stir at 95°C for 1h, centrifuge at 4000r / min, room temperature for 10min, collect the supernatant and concentrate, add D101 macroporous resin at a volume ratio of 2:1, decolorize at 180r / min for 2h; Collect the supernatant of the reagent, repeat 3 times, add 4 times the volume of abs...
Embodiment 2
[0045] Immunological and anti-erythrocyte hemolytic activity evaluation of selenized acidic polysaccharides from Rosa japonica:
[0046] (1) Determination of the immune activity of the selenized rosa japonica acid polysaccharide prepared in the embodiment of the present invention: dissolve the selenized polysaccharide in DMEM medium to make a 1000 μg / mL mother solution, and dilute it into 200 μg / mL and 100 μg / mL and 50μg / mL three dose groups; the original acidic polysaccharide was dissolved in DMEM medium to make a 200μg / mL solution; then the mouse macrophage RAW264.7 was thawed, and the thawed cells were quickly transferred to a 15mL sterile centrifuge tube, add 12mL DMEM medium, mix well and centrifuge at low speed (1000rpm / min, 5min). Remove the medium, add fresh medium, suspend the cells and transfer them to cell culture flasks. placed in CO 2 Cultured in an incubator (37°C, 5vol% CO 2 ), change the culture medium every two days, add 100 μ L of different concentrations...
Embodiment 3
[0049] A kind of preparation method of acidic polysaccharide of selenized golden cherry fruit
[0050] (1) Preparation of crude polysaccharides of Fructus Rosa Fructus: wash and dry the Fructus Rosa Fructus pulp, crush it and pass through a 100-mesh sieve to obtain the crushed Fructus Rosa Fructus pulp, and put it in a dry place for later use; condense the above crushed substance with 95% ethanol Reflux, the reflux temperature is 70°C, the reflux time is 2h, impurities and small molecular substances are removed, the supernatant is discarded by suction filtration, and the filter residue is naturally dried for later use; the filter residue is added with water at a ratio of 1:30 (g / mL), and the Stir at 95°C for 1h, centrifuge at 4000r / min for 10min at room temperature, collect the supernatant and concentrate, add D101 macroporous resin at a volume ratio of 3:1, decolorize at 160r / min for 1h; decolorize the sugar solution with 3 times the volume of Sevage Collect the supernatant o...
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