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Agrobacterium tumefaciens F-45 with high yield of C21 steroidal glycoside and application of Agrobacterium tumefaciens F-45

A technology of Agrobacterium tumefaciens and F-45, applied in the direction of bacteria, microorganism-based methods, microorganisms, etc., can solve the problems of low plant content and limited resources, and achieve good growth performance, wide application range, and reliable strain resources The effect of support

Active Publication Date: 2021-12-10
YANCHENG TEACHERS UNIV
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  • Application Information

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Problems solved by technology

[0004] The object of the present invention is to provide a kind of Agrobacterium tumefaciens F-45 and its efficient metabolism synthesis anticancer drug C21 steroids The application in the compound aims to solve the existing problems of limited resources and low plant body content in the extraction of anticancer drugs from plants

Method used

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  • Agrobacterium tumefaciens F-45 with high yield of C21 steroidal glycoside and application of Agrobacterium tumefaciens F-45
  • Agrobacterium tumefaciens F-45 with high yield of C21 steroidal glycoside and application of Agrobacterium tumefaciens F-45
  • Agrobacterium tumefaciens F-45 with high yield of C21 steroidal glycoside and application of Agrobacterium tumefaciens F-45

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Embodiment

[0027] 1. Isolation, purification and screening of endophytic actinomycetes from Radix Polygonum multiflorum

[0028] 1. Sample pretreatment

[0029] The Binhai Baishouwu plants used in the present invention are collected from the planting base of Guolaoshouwu in Binhai County, Jiangsu Province. After surface washing, disinfection and other treatments, the endophytic bacteria are immediately separated.

[0030] Wash the fresh Radix Polygoni multiflori plants, absorb the surface moisture, and sterilize them with ultraviolet light for 10 minutes in ultra-clean Taichung; rinse with sterile water 3 times, and absorb the surface moisture; soak the roots, stems, leaves, and seeds in 75% ethanol solution for 5 minutes each, Rinse 3 times with sterile water; then soak in 3% sodium hypochlorite solution for 3 minutes, rinse 5 times with sterile water, and keep the last rinse solution for disinfection inspection.

[0031] 2. Preparation of culture medium

[0032] Gaoshi No. 1 Medium: ...

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Abstract

The invention discloses Agrobacterium tumefaciens F-45 with the high yield of C21 steroidal glycoside and application of the Agrobacterium tumefaciens F-45. The endophytic actinomycetes are screened from coastal radix cynanchi bungei plants in a planting base of Fuli radix polygoni multiflori in coastal county of Jiangsu province, the strains are separated and purified by utilizing plate streaking, numbered and preserved, the used parent strains are subjected to physical character analysis, 16SrDNA primer amplification and sequence determination and analysis, and the identification result is agrobacterium tumefaciens F-45. The Agrobacterium tumefaciens F-45 is preserved in the China Center for Type Culture Collection in September 13, 2021, the preservation number is CCTCC NO: M 20211166, the preservation address is Wuhan University preservation center, Luoma, Wuchang District, Wuhan, and the postal code is 430072. the agrobacterium tumefaciens F-45 is short in growth cycle, easy in metabolism control, easy in strain breeding and large-scale fermentation industrial production, and can be used as a dominant strain for producing C21 steroid compounds.

Description

technical field [0001] The invention belongs to the field of biopharmaceuticals, in particular to a kind of high-efficiency metabolic synthesis of C 21 Steroids of Agrobacterium tumefaciens F-45 and their applications. Background technique [0002] Separation and screening of effective and safe new anti-cancer drugs from traditional Chinese medicines that have been proven to have anti-cancer effects has become a research hotspot. Baishouwu in C 21 Steroids are currently one of the main anticancer active ingredients, and they are also potential sources of anticancer drugs. However, due to the limitations of limited resources and low plant content, it is particularly important to explore its new biosynthetic pathway. Because of the advantages of endophytic actinomycetes in medicinal plants, such as abundant resources, diverse species, short growth cycle, easy metabolic control, easy strain selection and large-scale fermentation industrial production, etc. Therefore, in the...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P33/20C12R1/01
CPCC12P33/20
Inventor 王欢莉张言周洪键刘方方华舒恬金一诺
Owner YANCHENG TEACHERS UNIV
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